LOCUS       sequence-181830-        3325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: Nos, Gene: GFP, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A3014
FEATURES             Location/Qualifiers
     source          1..3325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        962..1141
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        994..1177
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(1219..1247)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1842..1862
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1913..2161
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        2233..2337
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccga tcatgagcgg agaattaagg
      901 gagtcacgtt atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac
      961 agaaccgcaa cgttgaagga gccactcagc cgcgggtttc tggagtttaa tgagctaagc
     1021 acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc
     1081 aaatatttct tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt
     1141 agagtctcat attcactctc aatccaaata atctgcacct gcaggatggt gagtaaagga
     1201 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1261 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     1321 aaatttattt gcactactgg aaaactacct gttccatggc caaccctggt caccaccctg
     1381 acctacggcg tgcagtgctt ctcccgttac cctgatcata tgaagcggca cgacttcttc
     1441 aagagcgcca tgcctgaggg atacgtgcag gagaggacca tcttcttcaa ggacgacggg
     1501 aactacaaga cacgtgctga agtcaagttt gagggagaca ccctcgtcaa caggatcgag
     1561 cttaagggaa tcgatttcaa ggaggacgga aacatcctcg gccacaagtt ggaatacaac
     1621 tacaactccc acaacgtata catcatggcc gacaagcaaa agaacggcat caaagccaac
     1681 ttcaagaccc gccacaacat cgaagacggc ggcgtgcaac tcgctgatca ttatcaacaa
     1741 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     1801 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     1861 acagctgctg ggattacaca tggcatggat gaactataca aataactcga gatatgaaga
     1921 tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt
     1981 tcttggcttg ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagatca
     2041 cattataatg aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt
     2101 ctgcagcata taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa
     2161 ggagctccgg tgacggacgt gggcaggtga tgaaaagcct tagggtcagg tggcactttt
     2221 cggggaaatg tgcgatgaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2281 ccgctaatga gacaataacc ctgataaatg cttcaattat attgaaaaag gaagagtatg
     2341 agtatccaac atttccgtgt cgcccttatt cccttttttg cggccttctg ccttcctgtt
     2401 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcccga
     2461 gtgggttaca ttgaactgga tctcaacagc ggcaaaatct tagagagttt tcgccccgaa
     2521 gaacgcttcc caatgatgag cactttcaaa gttctgctat gtggcgcggt gttatcccgt
     2581 attgatgctg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2641 gagtattcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgt
     2701 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgattgga
     2761 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2821 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2881 gtagcaatgg caacaactct ccgcaaacta ttaactggcg aactacttac tcttgcttca
     2941 cgccagcaac tcattgactg gatggaggcg gacaaagttg caggaccact tctgcgctcg
     3001 gcacttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3061 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3121 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3181 ctgattaagc attggtaact gtcagacgaa gggaaataaa tagtagcccg cctgatgtgc
     3241 gggctttttt ttggtggtgg ttaccagtgt cgcttagcta ccgtaggtcc tcttaggcgg
     3301 tccgattagc gacatagtca tcaat
//
