LOCUS       sequence-181877-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET probe; extracelllular synapse targeted mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Octeau JC, Chai H, Jiang R, Bonanno SL, Martin KC, Khakh BS
  TITLE     An Optical Neuron-Astrocyte Proximity Assay at Synaptic Distance 
            Scales.
  JOURNAL   Neuron. 2018 Apr 4;98(1):49-66.e9. doi: 
            10.1016/j.neuron.2018.03.003.
  PUBMED    29621490
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.03"; journalName: "Neuron"; date: "2018-04-4-
            4"; volume: "98"; issue: "1"; pages: "49-66.e9"
COMMENT     Sequence Label: pZac2.1 hSynapsin1 NAPA-N2 SV40
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     104..123
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        166..184
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(422..440)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     690..709
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaaaactc
       61 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      121 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
      181 taggctagcc tcgagaccac catgccacct tctactagtc ttttgttgct tgctgcactt
      241 ctccccttcg ctcttcccgc ttcagattgg aagacaggag aagtcaccat ggtgagcaag
      301 ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
      361 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      421 gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
      481 cagttcatgt acggctccag ggccttcacc aagcaccccg ccgacatccc cgactactat
      541 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      601 gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
      661 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg
      721 gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga cattaagatg
      781 gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac ctacaaggcc
      841 aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc gcaagttgga catcacctcc
      901 cacaacgagg actacaccg
//
