LOCUS       sequence-181908-        1359 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AT2R N terminal BRIL, linker GSGS, delta aa 1-34, delta aa 336-363.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Zhang H, Han GW, Batyuk A, Ishchenko A, White KL, Patel N, Sadybekov
            A, Zamlynny B, Rudd MT, Hollenstein K, Tolstikova A, White TA, 
            Hunter MS, Weierstall U, Liu W, Babaoglu K, Moore EL, Katz RD, 
            Shipman JM, Garcia-Calvo M, Sharma S, Sheth P, Soisson SM, Stevens 
            RC, Katritch V, Cherezov V
  TITLE     Structural basis for selectivity and diversity in angiotensin II 
            receptors.
  JOURNAL   Nature. 2017 Apr 20;544(7650):327-332. doi: 10.1038/nature22035. 
            Epub 2017 Apr 5.
  PUBMED    28379944
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature22035"; journalName: "Nature"; date: "2017-04-20- 
            20"; volume: "544"; issue: "7650"; pages: "327-332"
COMMENT     Sequence Label: IMPT-6213
FEATURES             Location/Qualifiers
     source          1..1359
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..72
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             73..102
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             103..123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgaagacaa tcatcgctct ctcttacatc ttctgcctcg tgttcgctga ctacaaggac
       61 gacgacgaca agcaccatca ccatcaccac caccatcacc acgagaactt gtacttccag
      121 ggagctgacc tggaggataa ctgggaaacc ctgaacgaca acctcaaggt tatcgaaaag
      181 gccgacaacg ctgcccaggt gaaggatgct ctgactaaga tgagggctgc cgctctcgac
      241 gcccaaaagg ctacaccccc taagctggag gacaagtcgc ccgattcccc tgaaatgaag
      301 gacttcagac acggtttcga tatcctggtc ggccaaatcg acgatgcctt gaagctggct
      361 aacgagggca aggtgaagga agcccaggcc gctgccgagc aactgaagac cactaggaac
      421 gcttacatcc agaagtacct cggttccggc tcttgctctc aaaagcctag cgacaagcac
      481 ctggatgcca tcccaatcct ctactacatc atcttcgtta tcggattctt ggtgaacatc
      541 gtggtcgtta ctctgttctg ctgtcagaag ggtccaaaga aggtctcctc tatctacatc
      601 ttcaacctcg ccgttgctga cctgctcttg ctggccacac tccctttgtg ggctacctac
      661 tactcttacc gttacgattg gctgttcgga ccagtgatgt gcaaggtctt cggttccttc
      721 ctgacactca acatgttcgc ttctatcttc ttcatcacct gtatgtcagt ggaccgctac
      781 cagtcggtta tctacccatt cctcagccag cgtcgcaacc cgtggcaagc ctcatacatc
      841 gttcccttgg tgtggtgcat ggcttgtctc agctcattgc cgactttcta cttcagggac
      901 gtgagaacaa tcgagtacct gggcgtcaac gcctgtatca tggctttccc accggaaaag
      961 tacgcccagt ggtctgccgg catcgctttg atgaagaaca tcctgggatt catcatccct
     1021 ctgatcttca tcgctacctg ctacttcggt atccgtaagc acctcttgaa gaccaacagc
     1081 tacggcaaga accgtatcac tcgcgaccaa gtcctgaaga tggctgccgc tgtggtcctc
     1141 gccttcatca tctgctggtt gcccttccac gtcctgacct tcctcgacgc cttggcttgg
     1201 atgggcgtga tcaactcgtg tgaggtcatc gctgttatcg atttggccct gccgttcgct
     1261 atcctgctcg gattcactaa ctcatgcgtg aaccccttcc tctactgttt cgttggtaac
     1321 cgtttccagc agaagttgag gagcgttttc cgtgtctaa
//
