LOCUS       sequence-181919-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse CBP TAZ2 domain by coexpression with 
            HisGB1 tagged STAT1 TAD .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Wojciak JM, Martinez-Yamout MA, Dyson HJ, Wright PE
  TITLE     Structural basis for recruitment of CBP/p300 coactivators by STAT1 
            and STAT2 transactivation domains.
  JOURNAL   EMBO J. 2009 Apr 8;28(7):948-58. doi: 10.1038/emboj.2009.30. Epub 
            2009 Feb 12.
  PUBMED    19214187
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2009.30"; journalName: "EMBO J"; date: "2009-04-8- 
            8"; volume: "28"; issue: "7"; pages: "948-58"
COMMENT     Sequence Label: HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             42..203
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             228..245
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             400..422
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             738..755
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccatgggcca tcatcatcat catcacagca gcggcatgac ttacaaactg atccttaacg
       61 gtaaaacttt gaaaggcgaa accactaccg aagctgttga tgctgcgact gcagaaaaag
      121 ttttcaaaca gtacgctaac gacaacggtg ttgacggtga atggacttac gacgatgcga
      181 ctaagacctt caccgttact gaagaattca gcagcggcag cagcggcctg gttccgcgtg
      241 gttcccatat gtctgaagtt cacccttcta gacttcagac cacagacaac ctgctcccca
      301 tgtctcctga ggagtttgac gaggtgtctc ggatagtggg ctctgtagaa ttcgacagta
      361 tgatgaacac agtataatag gatcccctct agaaataatt ttgtttaact ttaagaagga
      421 gatataccat gggaagccca caggaatccc ggcgtctcag catccagcgc tgcatccagt
      481 ccctggtgca tgcctgccag tgtcgcaatg ccaactgctc actgccgtct tgccagaaga
      541 tgaagcgagt cgtgcagcac accaagggct gcaagcgcaa gactaatgga ggatgcccag
      601 tgtgcaagca gctcattgct ctttgctgct accacgccaa acactgccaa gaaaataaat
      661 gccctgtgcc cttctgcctc aacatcaaac ataagctccg ccagcagcag taatagaagc
      721 ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      781 gaaagaagcg atgc
//
