LOCUS       sequence-182046-        2942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: none – to be combined with gRNA array in module 
            B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with 
            Csy4 spacers (only works with pMOD_B2203), Terminator: 35S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C2200
FEATURES             Location/Qualifiers
     source          1..2942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             1198..1503
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1382..1401
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     polyA_signal    1531..1705
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        1850..1954
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcgggaga gcgcccacct gccagggcag cgaagagcaa gccagataac agtatgcgta
      901 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
      961 aaaaggaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     1021 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     1081 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     1141 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     1201 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     1261 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     1321 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     1381 cgcatgatga ccaccgatat ggccagtgtg ccggtttccg ttatcgggga agaagtggct
     1441 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     1501 taagctcttc agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc
     1561 agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc
     1621 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     1681 aaaatccagt actaaaatcc agatcccccg aattagagct cgcgcgtagt cctcggtaat
     1741 atcgcagaac aaaagtacct gatatcgagt gtacttcaag tcacaccggc gagtgactag
     1801 caggtgatga aaagccttag ggtcaggtgg cacttttcgg ggaaatgtgc gatgaacccc
     1861 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctaatgagac aataaccctg
     1921 ataaatgctt caattatatt gaaaaaggaa gagtatgagt atccaacatt tccgtgtcgc
     1981 ccttattccc ttttttgcgg ccttctgcct tcctgttttt gctcacccag aaacgctggt
     2041 gaaagtaaaa gatgctgaag atcagttggg tgcccgagtg ggttacattg aactggatct
     2101 caacagcggc aaaatcttag agagttttcg ccccgaagaa cgcttcccaa tgatgagcac
     2161 tttcaaagtt ctgctatgtg gcgcggtgtt atcccgtatt gatgctgggc aagagcaact
     2221 cggtcgccgc atacactatt ctcagaatga cttggttgag tattcaccag tcacagaaaa
     2281 gcatcttacg gatggcatga cagtaagaga attatgtagt gctgccataa ccatgagtga
     2341 taacactgcg gccaacttac ttctgacaac gattggagga ccgaaggagc taaccgcttt
     2401 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2461 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caactctccg
     2521 caaactatta actggcgaac tacttactct tgcttcacgc cagcaactca ttgactggat
     2581 ggaggcggac aaagttgcag gaccacttct gcgctcggca cttccggctg gctggtttat
     2641 tgctgataaa tctggagccg gtgagcgtgg ctctcgcggt atcattgcag cactggggcc
     2701 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2761 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2821 agacgaaggg aaataaatag tagcccgcct gatgtgcggg cttttttttg gtggtggtta
     2881 ccagtgtcgc ttagctaccg taggtcctct taggcggtcc gattagcgac atagtcatca
     2941 at
//
