LOCUS       sequence-182138-       11156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral 
            Replication: none.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 11156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_260d
FEATURES             Location/Qualifiers
     source          1..11156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1793..2344)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     protein_bind    4284..4305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4320..4325,4326..4343,4344..4350)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4358..4374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4363..4385
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4382..4398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4382..4398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4444..4568
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4593..4598,4599..4616,4617..4623)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4677..5357
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5677..5982
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPASVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5861..5880
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6023..6147)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6167..6184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6167..6183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6176..6198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6386..6410
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7711..8340
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8769..9842
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9908..10102
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10338..10360)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10446..10586
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10501..10520
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10601..10618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10772..11156,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10852..10871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct
     1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
     1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca
     2041 gtcccgtccg ctggtggcgg gggctgacgt acacggtgga ctcggccgtc cagtcgtagg
     2101 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     2341 tcataagctt ctgcaaaaga gaaccagaca acagggtaag tgcctagcag taaacaaaca
     2401 gaactcatca caagcaaaca gcaacatcat attcatacca acaggtcatg tgtgttcatc
     2461 acatcattag tactaagcat gccatcatcc aagtatatca aagtaagggc aaagagcatg
     2521 catgatcatc aggtgcacaa aagaatcatc aaattgtagc agtacatatc ttcatctatc
     2581 atgcatatct atccataaca ggacgatgca tgttgaccag gtaaaagcta caggatccta
     2641 taggaacagc agcgtatata tcttcaccaa tcttagcatc ttaatcatgt ggcacatgca
     2701 gtttcaattt aagcacatga gctagttgat tatgaggtac cagagaatca tcaaactgat
     2761 gtagcagcat atatcctcat ctatcatgca atctaatcta atctaactaa acaggaaagg
     2821 tgtgctattc agttaaaagc taccgcatca tatacaaacg gcagcataag aaaaagcata
     2881 atcatcttaa tcatgcaaca aacgcagatt cataataagc ccaagagcta gcttgtgatg
     2941 atcttattct actctgatct acagcaatca gataacgacc taaccttgca catggcaaca
     3001 aaacaatcga tcggacgaat cagttgtttg ttcctagcta gcaccatcga accagataat
     3061 agatgcacgt acagatcccg aaaacgaacc caaaaacagg gcagacctag ctgaacctag
     3121 gcagcgaccc agcagatcgt gagaacgatc tcatctacga acagcctaga agcaacccca
     3181 cgattcccgg acaaacgacc taaaatcccc acaaatcaca tgagcatgac aggataaaca
     3241 gcggaaccga tcagatctac acgaaaaccc cacctcccag ccacccacga tcaggaaaca
     3301 cgcggatcta gcatgatttc gtcaacgcct cagcctagtt cctagccaca gaccaagcag
     3361 aaccaccaaa ccacgccgag cgaggagatg gggcaagagg acgggggaga cgatcgccgt
     3421 accttgaagc gggggaagga tcgccgaggg tcgcgaggag agcaatttgg atttggaacc
     3481 cgggggttgt gcgctccgaa cgatgagacg atgtgagatt gtgggaagag gcgcggaggg
     3541 ccctgtattt atgggctgcg acggggggag gagaggtggg gagggttggg gaaggaatcc
     3601 cccacccgtg ccgtgacggt tccgggccgt gtgagaggag cccgctcgtc tccgccacgc
     3661 aatttccgcg atcggagcgg agctttcgag aggcggctgg atggttggtg gccgttagat
     3721 ttgtagacgc cgttaacgcc tcgcctccac cgggaagagt tttgagcagc cgcttatgac
     3781 aatggcttaa cgacgttaga cggagcgtta gtggcaggcc atgatagaat agacgtattg
     3841 caatgggatt ataattaaat aaataagaat ataataagat atggcaagtc ggcactcatg
     3901 acatggtctt cgaaatgata gtgctcactt tcttagccga gaaagttgac gcgactgatt
     3961 tagaagttaa gatattattt ctctcttctt ttctttcctc gtcatataag gatgaaataa
     4021 actttagaga ttgccggtag tgattttgga tttcggcgat caggcttggt ttgccggttt
     4081 cggacggtgt gccttaggcc acccgcagtg tatcttgtaa tgttcaaccg ataagcaagg
     4141 gtggggctca agcaagtagt aaacaactat gtcaaatgtc accatggtta tggtcttgtt
     4201 tagttggctt cttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     4261 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     4321 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     4381 acaggaaaca gctatgacat gattacgaat tcgagctcgg tacccgggga tcaaaagcag
     4441 gtgacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     4501 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     4561 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     4621 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     4681 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     4741 ttgaggcatt tcagtcagtc gctcaatgta cctataacca gaccgttcag ctggatatta
     4801 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     4861 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     4921 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     4981 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     5041 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     5101 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     5161 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     5221 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     5281 gcagaatgct taatgaatta caacagtact gcgatgagtg gcaggcgggg cgtaatctag
     5341 aggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     5401 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     5461 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     5521 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     5581 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     5641 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     5701 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     5761 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtcccccgt
     5821 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     5881 gccagtgtgc cggcctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     5941 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     6001 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
     6061 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     6121 tcgttcagct ttcttgtaca aagtggtcac ctgcaaaaag tgtggcactg gccgtcgttt
     6181 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6241 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6301 tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc
     6361 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     6421 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     6481 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     6541 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
     6601 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     6661 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     6721 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     6781 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     6841 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     6901 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     6961 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     7021 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     7081 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     7141 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     7201 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     7261 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     7321 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     7381 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     7441 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     7501 gcccgcgcac ggctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     7561 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     7621 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     7681 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     7741 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     7801 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     7861 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     7921 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     7981 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     8041 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     8101 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     8161 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     8221 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     8281 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     8341 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     8401 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     8461 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     8521 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     8581 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     8641 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     8701 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
     8761 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     8821 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     8881 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     8941 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     9001 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     9061 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     9121 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     9181 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     9241 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     9301 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     9361 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     9421 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     9481 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
     9541 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
     9601 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     9661 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     9721 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
     9781 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
     9841 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggcc
     9901 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
     9961 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
    10021 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
    10081 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agcccaagag ctgcaaaaag
    10141 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
    10201 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
    10261 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
    10321 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg aaacggtcac agcttgtctg
    10381 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    10441 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
    10501 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10561 gcgtaaggag aaaataccgc atcaggccct cttccgcttc ctcgctcact gactcgctgc
    10621 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10681 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10741 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10801 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10861 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10921 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10981 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    11041 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    11101 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtat
//
