LOCUS       sequence-182153-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3XFlag::tagBFP::linker::TEV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP998-5
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..81
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             82..777
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             829..849
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cggtacccgg ggatcgacta caaagaccat gacggtgatt ataaagatca tgatatcgat
       61 tacaaggatg acgatgacaa gagcgagctg attaaggaga acatgcacat gaagctgtac
      121 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgaaggcga aggcaagccc
      181 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
      241 ttcgacatcc tggcgactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
      301 ggcatccccg acttcttcaa gcagtccttc cctgaaggct tcacatggga gagagtcacc
      361 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
      421 ctcatataca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
      481 aagaaaacac tcggctggga ggccttcacc gaaacgctgt accccgctga cggcggcctg
      541 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc
      601 aaaaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
      661 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
      721 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaattgc
      781 ccgggggatc ggtggagctc caccggtggc ggccgctcta gaactagtga gaacctctac
      841 ttccaaggag atcctctaga gtcg
//
