LOCUS       sequence-182178-        6436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. cerevisiae expression of F-box protein TIR1-9myc under control of
            the ADH1 promoter, TRP1 marker (for use with the AID degron system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6436)
  TITLE     Unpublished Ulrich lab AID plasmids
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YIp204-PADH1-atTIR1-9myc
FEATURES             Location/Qualifiers
     source          1..6436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..29)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(263..282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(942..1733,1734..1802))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1565..1584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1803..1907)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1975..1993
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1995..2096
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             2097..2771
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(2890..2912)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3012..3031
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3225..3247
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3239..3256
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3240..3256
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3549..3950
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5778..5807
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5814..5843
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5850..5879
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5898..5927
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5934..5963
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5970..5999
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6018..6047
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6054..6083
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6090..6119
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6217..6233)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6217..6233)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6230..6252)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6241..6257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6265..6271,6272..6289,6290..6295))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6310..6331
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1981 cgtatcacga ggccaattcg gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt
     2041 ggtgactatt gagcacgtga gtatacgtga ttaagcacac aaaggcagct tggagtatgt
     2101 ctgttattaa tttcacaggt agttctggtc cattggtgaa agtttgcggc ttgcagagca
     2161 cagaggccgc agaatgtgca ctagattccg atgctgactt gctgggtatt atatgtgtgc
     2221 ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag
     2281 catataaaaa tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta
     2341 aggaggatgt tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag
     2401 atgagtcgtg gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat
     2461 ttccaaaaga ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc
     2521 ccttgtttga ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg
     2581 actgggttgg aaggcaagag agccccgaga gcttacattt tatgttagct ggtggactga
     2641 cgccagaaaa tgttggtgat gcgcttagat taaatggcgt tattggtgtt gatgtaagcg
     2701 gaggtgtgga gacaaatggt gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg
     2761 ctaagaaata ggttattact gagtagtatt tatttaagta ttgtttgtgc acttgcctgc
     2821 aagccttttg aaaagcaagc ataaaagatc gccctttcgt ctcgcgcgtt tcggtgatga
     2881 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     2941 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     3001 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     3061 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     3121 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     3181 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     3241 taaaacgacg gccagtgaat tcgggtgtac aatatggact tcctcttttc tggcaaccaa
     3301 acccatacat cgggattcct ataatacctt cgttggtctc cctaacatgt aggtggcgga
     3361 ggggagatat acaatagaac agataccaga caagacataa tgggctaaac aagactacac
     3421 caattacact gcctcattga tggtggtaca taacgaacta atactgtagc cctagacttg
     3481 atagccatca tcatatcgaa gtttcactac cctttttcca tttgccatct attgaagtaa
     3541 taataggcgc atgcaacttc ttttcttttt ttttcttttc tctctccccc gttgttgtct
     3601 caccatatcc gcaatgacaa aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa
     3661 agacagcacc aacagatgtc gttgttccag agctgatgag gggtatctcg aagcacacga
     3721 aactttttcc ttccttcatt cacgcacact actctctaat gagcaacggt atacggcctt
     3781 ccttccagtt acttgaattt gaaataaaaa aaagtttgct gtcttgctat caagtataaa
     3841 tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt
     3901 gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact atctcatata
     3961 caactagtat gcagaagcga atagccttgt cgtttccaga agaggtacta gagcatgtgt
     4021 tctcgtttat tcagctggat aaggatagga actcagtctc tctggtgtgc aagtcatggt
     4081 acgagatcga gcggtggtgc aggaggaaag tcttcatcgg gaactgctac gccgtgagtc
     4141 cagcgacggt gattaggagg ttcccgaaag tgagatccgt ggagcttaaa ggaaaacctc
     4201 actttgctga ctttaatttg gtacctgacg gatggggagg ttacgtgtat ccatggattg
     4261 aggccatgtc ttcgtcttac acgtggcttg aagagataag gctgaagagg atggtggtca
     4321 ccgacgattg cttggagctc atagccaagt cttttaagaa ttttaaggtt cttgtgcttt
     4381 cttcctgcga aggcttctcc accgatggtc tcgctgctat cgctgccact tgcaggaatc
     4441 tgaaagagct tgacttacga gagagtgatg ttgacgacgt tagtggccac tggcttagcc
     4501 atttcccaga tacatacact tctttggtat cactcaatat atcttgctta gcatctgagg
     4561 tcagtttctc tgctctggaa aggctggtga ctaggtgtcc caatctcaag tctctcaagc
     4621 ttaaccgagc tgttccactt gaaaaattgg ctactttact tcaaagagca cctcaattgg
     4681 aggaattggg cactggtggg tacactgcag aagtgcgacc agatgtttac tctggtttat
     4741 ctgtagcgct ctctgggtgc aaggaattga ggtgcttatc tggattttgg gatgctgttc
     4801 ctgcctatct tccagcagtt tattcggttt gcagtcggct tacaactttg aatctgagtt
     4861 atgcaacagt ccagagctat gatcttgtca agcttctttg tcaatgccct aaactgcagc
     4921 gcctctgggt gcttgactac atcgaggatg ctggtcttga ggtgcttgct tcaacctgca
     4981 aggacctacg cgagctgaga gtgtttccgt ccgagccttt tgtcatggaa ccaaatgtgg
     5041 cattgacgga acaggggctt gtctccgtct ccatgggctg tccaaaactc gagtcggttc
     5101 tctacttctg ccgtcaaatg accaatgctg cattgataac cattgctagg aaccgtccca
     5161 acatgactcg cttccgtttg tgcatcattg agccaaaagc cccagactat ctgactctag
     5221 agccactgga tattggattt ggagccatag tagagcactg caaggatctc cgtcgcctct
     5281 ctctatctgg cctcttgacc gacaaggttt ttgaatacat tgggacatat gccaagaaga
     5341 tggaaatgct ctcagtggca tttgcaggag acagtgactt aggcatgcat catgttttgt
     5401 ccgggtgcga tagcttgagg aaactagaga taagggactg cccgtttgga gacaaggcgc
     5461 ttttggccaa tgcttcaaag ctggagacaa tgcgatccct ttggatgtct tcttgttccg
     5521 tgagttttgg agcctgcaag ttactaggac agaagatgcc aaagctgaat gtggaagtca
     5581 tcgatgaacg gggtgcaccg gactcgagac cagagagctg ccctgttgag agagtcttca
     5641 tataccgaac agtggctggt cctcgatttg acatgcctgg cttcgtctgg aacatggacc
     5701 aagactcaac aatgaggttt tccaggcaaa tcattactac taacggatta gtcgactccg
     5761 gttctgctgc tagtggtgaa caaaagttga tttctgaaga agatttgaac ggggaacaaa
     5821 agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga
     5881 acggatcctc tagaggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa
     5941 agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga
     6001 acggatcctc tagaggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa
     6061 agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga
     6121 acggatccac tagctaaaaa gtggaacgat cattcaagag atccccgggg atcctctaga
     6181 gtcgacctgc aggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6241 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6301 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6361 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6421 tttgcgtatt gggcgc
//
