LOCUS       sequence-182248-        8995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + 
            CmYLCV:gRNAs with tRNA spacers , Plant Selection: none.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_10E
FEATURES             Location/Qualifiers
     source          1..8995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      27..615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     516..535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     769..786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1332..1677
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1620..1640
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1711..5814
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1711..5814
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5827..5847
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5858..6106
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        6689..6764
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             7161..7466
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7345..7364
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     polyA_signal    7494..7668
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             8138..8929
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
       61 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
      181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
      721 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
      781 agcgaggaag cgggagagcg cccatatgcg cactcctcgc atgcggcgcg ccgatcggcg
      841 cgccagattt gccttttcaa tttcagaaag aatgctaacc cacagatggt tagagaggct
      901 tacgcagcag gtatcatcaa gacgatctac ccgagcaata atctccagga aatcaaatac
      961 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat caagaacaca
     1021 gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
     1081 cttcacaaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcccactgaa
     1141 tcaaaggcca tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact
     1201 ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac
     1261 atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac
     1321 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat
     1381 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa
     1441 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc
     1501 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     1561 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     1621 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg
     1681 gggactcctg caggtagatc gctcgtcgac atggataaga agtactctat cggactcgat
     1741 atcggaacta actctgtggg atgggctgtg atcaccgatg agtacaaggt gccatctaag
     1801 aagttcaagg ttctcggaaa caccgatagg cactctatca agaaaaacct tatcggtgct
     1861 ctcctcttcg attctggtga aactgctgag gctaccagac tcaagagaac cgctagaaga
     1921 aggtacacca gaagaaagaa caggatctgc tacctccaag agatcttctc taacgagatg
     1981 gctaaagtgg atgattcatt cttccacagg ctcgaagagt cattcctcgt ggaagaagat
     2041 aagaagcacg agaggcaccc tatcttcgga aacatcgttg atgaggtggc ataccacgag
     2101 aagtacccta ctatctacca cctcagaaag aagctcgttg attctactga taaggctgat
     2161 ctcaggctca tctacctcgc tctcgctcac atgatcaagt tcagaggaca cttcctcatc
     2221 gagggtgatc tcaaccctga taactctgat gtggataagt tgttcatcca gctcgtgcag
     2281 acctacaacc agcttttcga agagaaccct atcaacgctt caggtgtgga tgctaaggct
     2341 atcctctctg ctaggctctc taagtcaaga aggcttgaga acctcattgc tcagctccct
     2401 ggtgagaaga agaacggact tttcggaaac ttgatcgctc tctctctcgg actcacccct
     2461 aacttcaagt ctaacttcga tctcgctgag gatgcaaagc tccagctctc aaaggatacc
     2521 tacgatgatg atctcgataa cctcctcgct cagatcggag atcagtacgc tgatttgttc
     2581 ctcgctgcta agaacctctc tgatgctatc ctcctcagtg atatcctcag agtgaacacc
     2641 gagatcacca aggctccact ctcagcttct atgatcaaga gatacgatga gcaccaccag
     2701 gatctcacac ttctcaaggc tcttgttaga cagcagctcc cagagaagta caaagagatt
     2761 ttcttcgatc agtctaagaa cggatacgct ggttacatcg atggtggtgc atctcaagaa
     2821 gagttctaca agttcatcaa gcctatcctc gagaagatgg atggaaccga ggaactcctc
     2881 gtgaagctca atagagagga tcttctcaga aagcagagga ccttcgataa cggatctatc
     2941 cctcatcaga tccacctcgg agagttgcac gctatcctta gaaggcaaga ggatttctac
     3001 ccattcctca aggataacag ggaaaagatt gagaagattc tcaccttcag aatcccttac
     3061 tacgtgggac ctctcgctag aggaaactca agattcgctt ggatgaccag aaagtctgag
     3121 gaaaccatca ccccttggaa cttcgaagag gtggtggata agggtgctag tgctcagtct
     3181 ttcatcgaga ggatgaccaa cttcgataag aaccttccaa acgagaaggt gctccctaag
     3241 cactctttgc tctacgagta cttcaccgtg tacaacgagt tgaccaaggt taagtacgtg
     3301 accgagggaa tgaggaagcc tgcttttttg tcaggtgagc aaaagaaggc tatcgttgat
     3361 ctcttgttca agaccaacag aaaggtgacc gtgaagcagc tcaaagagga ttacttcaag
     3421 aaaatcgagt gcttcgattc agttgagatt tctggtgttg aggataggtt caacgcatct
     3481 ctcggaacct accacgatct cctcaagatc attaaggata aggatttctt ggataacgag
     3541 gaaaacgagg atatcttgga ggatatcgtt cttaccctca ccctctttga agatagagag
     3601 atgattgaag aaaggctcaa gacctacgct catctcttcg atgataaggt gatgaagcag
     3661 ttgaagagaa gaagatacac tggttgggga aggctctcaa gaaagctcat taacggaatc
     3721 agggataagc agtctggaaa gacaatcctt gatttcctca agtctgatgg attcgctaac
     3781 agaaacttca tgcagctcat ccacgatgat tctctcacct ttaaagagga tatccagaag
     3841 gctcaggttt caggacaggg tgatagtctc catgagcata tcgctaacct cgctggatct
     3901 cctgcaatca agaagggaat cctccagact gtgaaggttg tggatgagtt ggtgaaggtg
     3961 atgggaaggc ataagcctga gaacatcgtg atcgaaatgg ctagagagaa ccagaccact
     4021 cagaagggac agaagaactc tagggaaagg atgaagagga tcgaggaagg tatcaaagag
     4081 cttggatctc agatcctcaa agagcaccct gttgagaaca ctcagctcca gaatgagaag
     4141 ctctacctct actacctcca gaacggaagg gatatgtatg tggatcaaga gttggatatc
     4201 aacaggctct ctgattacga tgttgatcat atcgtgccac agtcattctt gaaggatgat
     4261 tctatcgata acaaggtgct caccaggtct gataagaaca ggggtaagag tgataacgtg
     4321 ccaagtgaag aggttgtgaa gaaaatgaag aactattgga ggcagctcct caacgctaag
     4381 ctcatcactc agagaaagtt cgataacttg actaaggctg agaggggagg actctctgaa
     4441 ttggataagg caggattcat caagaggcag cttgtggaaa ccaggcagat cactaagcac
     4501 gttgcacaga tcctcgattc taggatgaac accaagtacg atgagaacga taagttgatc
     4561 agggaagtga aggttatcac cctcaagtca aagctcgtgt ctgatttcag aaaggatttc
     4621 caattctaca aggtgaggga aatcaacaac taccaccacg ctcacgatgc ttaccttaac
     4681 gctgttgttg gaaccgctct catcaagaag tatcctaagc tcgagtcaga gttcgtgtac
     4741 ggtgattaca aggtgtacga tgtgaggaag atgatcgcta agtctgagca agagatcgga
     4801 aaggctaccg ctaagtattt cttctactct aacatcatga atttcttcaa gaccgagatt
     4861 accctcgcta acggtgagat cagaaagagg ccactcatcg agacaaacgg tgaaacaggt
     4921 gagatcgtgt gggataaggg aagggatttc gctaccgtta gaaaggtgct ctctatgcca
     4981 caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg gattctctaa agagtctatc
     5041 ctccctaaga ggaactctga taagctcatt gctaggaaga aggattggga ccctaagaaa
     5101 tacggtggtt tcgattctcc taccgtggct tactctgttc tcgttgtggc taaggttgag
     5161 aagggaaaga gtaagaagct caagtctgtt aaggaacttc tcggaatcac tatcatggaa
     5221 aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg ctaagggata caaagaggtt
     5281 aagaaggatc tcatcatcaa gctcccaaag tactcactct tcgaactcga gaacggtaga
     5341 aagaggatgc tcgcttctgc tggtgagctt caaaagggaa acgagcttgc tctcccatct
     5401 aagtacgtta actttcttta cctcgcttct cactacgaga agttgaaggg atctccagaa
     5461 gataacgagc agaagcaact tttcgttgag cagcacaagc actacttgga tgagatcatc
     5521 gagcagatct ctgagttctc taaaagggtg atcctcgctg atgcaaacct cgataaggtg
     5581 ttgtctgctt acaacaagca cagagataag cctatcaggg aacaggcaga gaacatcatc
     5641 catctcttca cccttaccaa cctcggtgct cctgctgctt tcaagtactt cgatacaacc
     5701 atcgatagga agagatacac ctctaccaaa gaagtgctcg atgctaccct catccatcag
     5761 tctatcactg gactctacga gactaggatc gatctctcac agctcggtgg tgattcaagg
     5821 gctgatccta agaagaagag gaaggtttga ctcgagatat gaagatgaag atgaaatatt
     5881 tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg gcttgttgtg
     5941 ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatcacatta taatgaataa
     6001 acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca gcatataact
     6061 actgtatgtg ctatggtatg gactatggaa tatgattaaa gataaggagc tccggtgacg
     6121 gacggcgcgc cgaagagctg gcagacatac tgtcccacaa atgaagatgg aatctgtaaa
     6181 agaaaacgcg tgaaataatg cgtctgacaa aggttaggtc ggctgccttt aatcaatacc
     6241 aaagtggtcc ctaccacgat ggaaaaactg tgcagtcggt ttggcttttt ctgacgaaca
     6301 aataagattc gtggccgaca ggtgggggtc caccatgtga aggcatcttc agactccaat
     6361 aatggagcaa tgacgtaagg gcttacgaaa taagtaaggg tagtttggga aatgtccact
     6421 cacccgtcag tctataaata cttagcccct ccctcattgt taagggagca aaatctcaga
     6481 gagatagtcc tagagagaga aagagagcaa gtagcctaga agtagtcaag gcggcgaagt
     6541 attcaggcac gtggccagga agaagaaaag ccaagacgac gaaaacaggt aagagctaag
     6601 cttcctgcag gaacaaagca ccagtggtct agtggtagaa tagtaccctg ccacggtaca
     6661 gacccgggtt cgattcccgg ctggtgcagt tttagagcta gaaatagcaa gttaaaataa
     6721 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcaacaaa gcaccagtgg
     6781 tctagtggta gaatagtacc ctgccacggt acagacccgg gttcgattcc cggctggtgc
     6841 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     6901 gatatgtata cccgaagtat gtcaaaagga ggtatgctat gaagcagcgt attacagtga
     6961 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     7021 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     7081 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     7141 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     7201 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     7261 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     7321 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggttt
     7381 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     7441 ttaacctgat gttctgggga atataagctc ttcagtcgat cgacaagctc gagtttctcc
     7501 ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca
     7561 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     7621 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattacc
     7681 ggcgagcttt gggtacgtca cgtggctcga gcgcgtagtc ctcggtaata tcgcagaaca
     7741 aaagtacctg atatcgagtg tacttcaagt cacaccggcg agtgccagcc aggacagaaa
     7801 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     7861 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     7921 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     7981 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     8041 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     8101 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     8161 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     8221 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     8281 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     8341 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     8401 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     8461 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     8521 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     8581 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     8641 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     8701 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     8761 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     8821 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     8881 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     8941 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagac
//
