LOCUS       sequence-182284-       19461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: T-DNA, Engineering Reagent: 
            ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant 
            Selection: PvUbi2:bar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19461)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 19461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_26H
FEATURES             Location/Qualifiers
     source          1..19461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1793..2344)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     protein_bind    4284..4305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4320..4325,4326..4343,4344..4350)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4358..4374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4363..4385
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4382..4398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4382..4398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7015..7071
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7072..11175
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7072..11175
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11176..11223
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      11235..11483
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(12225..12236)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    13503..13522
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        13523..13598
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    13599..13618
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     CDS             13938..14243
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     14122..14141
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     polyA_signal    14271..14445
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(14472..14489)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14472..14488)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14481..14503)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    14691..14715
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             16016..16645
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             17074..18147
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      18213..18407
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(18643..18665)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    18751..18891
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     18806..18825
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(18906..18923)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(19077..19461,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19157..19176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct
     1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
     1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca
     2041 gtcccgtccg ctggtggcgg gggctgacgt acacggtgga ctcggccgtc cagtcgtagg
     2101 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     2341 tcataagctt ctgcaaaaga gaaccagaca acagggtaag tgcctagcag taaacaaaca
     2401 gaactcatca caagcaaaca gcaacatcat attcatacca acaggtcatg tgtgttcatc
     2461 acatcattag tactaagcat gccatcatcc aagtatatca aagtaagggc aaagagcatg
     2521 catgatcatc aggtgcacaa aagaatcatc aaattgtagc agtacatatc ttcatctatc
     2581 atgcatatct atccataaca ggacgatgca tgttgaccag gtaaaagcta caggatccta
     2641 taggaacagc agcgtatata tcttcaccaa tcttagcatc ttaatcatgt ggcacatgca
     2701 gtttcaattt aagcacatga gctagttgat tatgaggtac cagagaatca tcaaactgat
     2761 gtagcagcat atatcctcat ctatcatgca atctaatcta atctaactaa acaggaaagg
     2821 tgtgctattc agttaaaagc taccgcatca tatacaaacg gcagcataag aaaaagcata
     2881 atcatcttaa tcatgcaaca aacgcagatt cataataagc ccaagagcta gcttgtgatg
     2941 atcttattct actctgatct acagcaatca gataacgacc taaccttgca catggcaaca
     3001 aaacaatcga tcggacgaat cagttgtttg ttcctagcta gcaccatcga accagataat
     3061 agatgcacgt acagatcccg aaaacgaacc caaaaacagg gcagacctag ctgaacctag
     3121 gcagcgaccc agcagatcgt gagaacgatc tcatctacga acagcctaga agcaacccca
     3181 cgattcccgg acaaacgacc taaaatcccc acaaatcaca tgagcatgac aggataaaca
     3241 gcggaaccga tcagatctac acgaaaaccc cacctcccag ccacccacga tcaggaaaca
     3301 cgcggatcta gcatgatttc gtcaacgcct cagcctagtt cctagccaca gaccaagcag
     3361 aaccaccaaa ccacgccgag cgaggagatg gggcaagagg acgggggaga cgatcgccgt
     3421 accttgaagc gggggaagga tcgccgaggg tcgcgaggag agcaatttgg atttggaacc
     3481 cgggggttgt gcgctccgaa cgatgagacg atgtgagatt gtgggaagag gcgcggaggg
     3541 ccctgtattt atgggctgcg acggggggag gagaggtggg gagggttggg gaaggaatcc
     3601 cccacccgtg ccgtgacggt tccgggccgt gtgagaggag cccgctcgtc tccgccacgc
     3661 aatttccgcg atcggagcgg agctttcgag aggcggctgg atggttggtg gccgttagat
     3721 ttgtagacgc cgttaacgcc tcgcctccac cgggaagagt tttgagcagc cgcttatgac
     3781 aatggcttaa cgacgttaga cggagcgtta gtggcaggcc atgatagaat agacgtattg
     3841 caatgggatt ataattaaat aaataagaat ataataagat atggcaagtc ggcactcatg
     3901 acatggtctt cgaaatgata gtgctcactt tcttagccga gaaagttgac gcgactgatt
     3961 tagaagttaa gatattattt ctctcttctt ttctttcctc gtcatataag gatgaaataa
     4021 actttagaga ttgccggtag tgattttgga tttcggcgat caggcttggt ttgccggttt
     4081 cggacggtgt gccttaggcc acccgcagtg tatcttgtaa tgttcaaccg ataagcaagg
     4141 gtggggctca agcaagtagt aaacaactat gtcaaatgtc accatggtta tggtcttgtt
     4201 tagttggctt cttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     4261 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     4321 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     4381 acaggaaaca gctatgacat gattacgaat tcgagctcgg tacccgggga tctgcaggcg
     4441 cgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag cattgcatgt
     4501 ctaagttata aaaaattacc acatattttt tttgtcacac ttgtttgaag tgcagtttat
     4561 ctatctttat acatatattt aaactttact ctacgaataa tataatctat agtactacaa
     4621 taatatcagt gttttagaga atcatataaa tgaacagtta gacatggtct aaaggacaat
     4681 tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg tgttctcctt
     4741 tttttttgca aatagcttca cctatataat acttcatcca ttttattagt acatccattt
     4801 agggtttagg gttaatggtt tttatagact aattttttta gtacatctat tttattctat
     4861 tttagcctct aaattaagaa aactaaaact ctattttagt ttttttattt aataatttag
     4921 atataaaata gaataaaata aagtgactaa aaattaaaca aatacccttt aagaaattaa
     4981 aaaaactaag gaaacatttt tcttgtttcg agtagataat gccagcctgt taaacgccgt
     5041 cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc
     5101 agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc gctccaccgt
     5161 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
     5221 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
     5281 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
     5341 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
     5401 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccctctctac
     5461 cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg
     5521 tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga
     5581 cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg
     5641 ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca
     5701 tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt
     5761 catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt
     5821 ctagatcgga gtagaattaa ttctgtttca aactacctgg tggatttatt aattttggat
     5881 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
     5941 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
     6001 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
     6061 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
     6121 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
     6181 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
     6241 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
     6301 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
     6361 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
     6421 ttggtgttac ttcctgcagg aagcatggac cactacctcg acatcaggct caggccagac
     6481 ccagagttcc caccagccca gctcatgtcc gtcctcttcg gcaagctcca ccaggccctc
     6541 gtggcccagg gcggcgacag gatcggcgtg tccttcccag acctcgacga gtccaggtcc
     6601 aggctcggcg agaggctccg catccacgcc tccgccgacg acctcagggc cctcctcgcc
     6661 aggccgtggc tggagggcct cagggaccac ctccagttcg gcgagccagc cgtggtgcca
     6721 cacccaaccc catacaggca agtgtccagg gtgcaagcca agtccaaccc agagaggctc
     6781 aggaggaggc tcatgaggag gcacgacctc tccgaggaag aggccaggaa gcgcatccca
     6841 gacaccgtgg ccagggccct cgacctccca ttcgtgaccc tcaggtccca gtccaccggc
     6901 cagcacttcc gcctcttcat caggcacggc ccactccagg tgaccgccga ggagggcggc
     6961 tttacctgct acggcctctc caagggcggc ttcgtgccgt ggttcggctc cggcgccacc
     7021 aacttctccc tcctcaagca agccggcgac gtggaggaga acccaggccc aatggacaag
     7081 aagtactcga tcggcctcga catcgggacg aactcagttg gctgggccgt gatcaccgac
     7141 gagtacaagg tgccctctaa gaagttcaag gtcctgggga acaccgaccg ccattccatc
     7201 aagaagaacc tcatcggcgc tctcctgttc gacagcgggg agaccgctga ggctacgagg
     7261 ctcaagagaa ccgctaggcg ccggtacacg agaaggaaga acaggatctg ctacctccaa
     7321 gagattttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag
     7381 tctttcctcg tggaggagga taagaagcac gagcggcatc ccatcttcgg caacatcgtg
     7441 gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg
     7501 gactccaccg ataaggcgga cctcagactg atctacctcg ctctggccca catgatcaag
     7561 ttccgcggcc atttcctgat cgagggggat ctcaacccag acaacagcga tgttgacaag
     7621 ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgcc
     7681 tctggcgtgg acgcgaaggc tatcctgtcc gcgaggctct cgaagtccag gaggctggag
     7741 aacctgatcg ctcagctccc aggcgagaag aagaacggcc tgttcgggaa cctcatcgct
     7801 ctcagcctgg ggctcacccc gaacttcaag tcgaacttcg atctcgctga ggacgccaag
     7861 ctgcaactct ccaaggacac ctacgacgat gacctcgata acctcctggc ccagatcggc
     7921 gatcaatacg cggacctgtt cctcgctgcc aagaacctgt cggacgccat cctcctgtca
     7981 gatatcctcc gcgtgaacac cgagatcacg aaggctccac tctctgcctc catgatcaag
     8041 cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc
     8101 ccggagaagt acaaggagat tttcttcgat cagtcgaaga acggctacgc tgggtacatc
     8161 gacggcgggg cctcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg
     8221 gacggcacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga
     8281 accttcgata acggcagcat cccccaccaa atccatctcg gggagctgca cgccatcctg
     8341 agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc
     8401 ctgaccttca gaatcccata ctacgtcggc cctctcgcgc gggggaactc aagattcgct
     8461 tggatgaccc gcaagtctga ggagaccatc acgccgtgga acttcgagga ggtggtggac
     8521 aagggcgcta gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc
     8581 aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag
     8641 ctcacgaagg tgaagtacgt caccgagggc atgcgcaagc cagcgttcct gtccggggag
     8701 cagaagaagg ctatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa
     8761 ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtcgagat cagcggcgtt
     8821 gaggaccgct tcaacgccag cctcgggacc taccacgatc tcctgaagat catcaaggat
     8881 aaggacttcc tggacaacga ggagaacgag gatatcctgg aggacatcgt gctgaccctc
     8941 acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc
     9001 gatgacaagg tcatgaagca actcaagcgc cggagataca ccggctgggg gaggctgtcc
     9061 cgcaagctca tcaacggcat ccgggacaag cagtccggga agaccatcct cgacttcctc
     9121 aagagcgatg gcttcgccaa caggaacttc atgcaactga tccacgatga cagcctcacc
     9181 ttcaaggagg atatccaaaa ggctcaagtg agcggccagg gggactcgct gcacgagcat
     9241 atcgcgaacc tcgctggctc ccccgcgatc aagaagggca tcctccagac cgtgaaggtt
     9301 gtggacgagc tcgtgaaggt catgggccgg cacaagcctg agaacatcgt catcgagatg
     9361 gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc
     9421 atcgaggagg gcatcaagga gctggggtcc caaatcctca aggagcaccc agtggagaac
     9481 acccaactgc agaacgagaa gctctacctg tactacctcc agaacggcag ggatatgtac
     9541 gtggaccaag agctggatat caaccgcctc agcgattacg acgtcgatca tatcgttccc
     9601 cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac
     9661 cgcggcaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg
     9721 aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct
     9781 gagagaggcg ggctctcaga gctggacaag gccggcttca tcaagcggca gctggtcgag
     9841 accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac
     9901 gatgagaacg acaagctgat cagggaggtt aaggtgatca ccctgaagtc taagctcgtc
     9961 tccgacttca ggaaggattt ccagttctac aaggttcgcg agatcaacaa ctaccaccat
    10021 gcccatgacg cttacctcaa cgctgtggtc ggcaccgctc tgatcaagaa gtacccaaag
    10081 ctggagtccg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc
    10141 aagtcggagc aagagatcgg caaggctacc gccaagtact tcttctactc aaacatcatg
    10201 aacttcttca agaccgagat cacgctggcc aacggcgaga tccggaagag accgctcatc
    10261 gagaccaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc
    10321 cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggc
    10381 gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag
    10441 aaggactggg acccaaagaa gtacggcggg ttcgacagcc ctaccgtggc ctactcggtc
    10501 ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctc
    10561 ctggggatca ccatcatgga gaggtccagc ttcgagaaga acccaatcga cttcctggag
    10621 gccaagggct acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc
    10681 ttcgagctgg agaacggcag gaagagaatg ctggcttccg ctggcgagct ccagaagggg
    10741 aacgagctcg cgctgccaag caagtacgtg aacttcctct acctggcttc ccactacgag
    10801 aagctcaagg gcagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag
    10861 cattacctcg acgagatcat cgagcaaatc tccgagttca gcaagcgcgt gatcctcgcc
    10921 gacgcgaacc tggataaggt cctctccgcc tacaacaagc accgggacaa gcccatcaga
    10981 gagcaagcgg agaacatcat ccatctcttc accctgacga acctcggcgc tcctgctgct
    11041 ttcaagtact tcgacaccac gatcgatcgg aagagataca cctccacgaa ggaggtcctg
    11101 gacgcgaccc tcatccacca gtcgatcacc ggcctgtacg agacgaggat cgacctctca
    11161 caactcggcg gggataagag acccgcagca accaagaagg cagggcaagc aaagaagaag
    11221 aagtgactcg agtatatgaa gatgaagatg aaatatttgg tgtgtcaaat aaaaagcttg
    11281 tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg gctttttcta
    11341 atattaaatg aatgtaagat cacattataa tgaataaaca aatgtttcta taatccattg
    11401 tgaatgtttt gttggatctc ttctgcagca tataactact gtatgtgcta tggtatggac
    11461 tatggaatat gattaaagat aaggagctcg ttaaccggtg acggacggcg cgccgaagag
    11521 cacgtcagtg tttggtttcc actagcacga gtagcgcaat cagaaaattt tcaatgcatg
    11581 aagtactaaa cgaagtttat ttagaaattt ttttaagaaa tgagtgtaat tttttgcgac
    11641 gaatttaatg acaataatta atcgatgatt gcctacagta atgctacagt aaccaacctc
    11701 taatcatgcg tcgaatgcgt cattagattc gtctcgcaaa atagcacaag aattatgaaa
    11761 ttaattttac aaactatttt tatttaatac taataattaa ctgtcaaagt ttgtgctact
    11821 cgcaagagta gcgcgaacca aacacggcct ggaggagcac ggtaacggcg tcgacaaact
    11881 aacggccacc acccgccaac gcaaaggaga cggatgagag ttgacttctt gacggttctc
    11941 cacccctctg tctctctgtc actgggccct gggtccccct ctcgaaagtt cctctggccg
    12001 aaattgcgcg gcggagacga ggcgggcgga accgtcacgg cagaggattc cttccccacc
    12061 ctgcctggcc cggccatata taaacagcca ccgcccctcc ccgttcccca tcgcgtctcg
    12121 tctcgtgttg ttcccagaac acaaccaaaa tccaaatcct cctcctcctc ccgagcctcg
    12181 tcgatccctc acccgcttca aggtacggcg atcctcctct cccttctccc ctcgatcgat
    12241 tatgcgtgtt ccgtttccgt ttccgatcga gcgaatcgat ggttaggacc catgggggac
    12301 ccatggggtg tcgtgtggtg gtctggtttg atccgcgata tttctccgtt cgtagtgtag
    12361 atctgatcga atccctggtg aaatcgttga tcgtgctatt cgtgtgaggg ttcttaggtt
    12421 tggagttgtg gaggtagttc tgatcggttt gtaggtgaga ttttccccat gattttgctt
    12481 ggctcgtttg tcttggttag attagatctg cccgcatttt gttcgatatt tctgatgcag
    12541 atatgatgaa taatttcgtc cttgtatccc gcgtccgtat gtgtattaag tttgcaggtc
    12601 ctagttaggt ttttcctact gatttgtctt atccattctg tttagcttgc aaggtttggt
    12661 aatggtccgg catgtttgtc tctatagatt agagtagaat aagattatct caacaagctg
    12721 ttggcttatc aattttggat ctgcatgtgt ttcgcatcta tatctttgca attaagatgg
    12781 tagatggaca tatgctcctg ttgagttgat gttgtacctt ttacctgagg tctgaggaac
    12841 atgcatcctc ctgctacttt gtgcttatac agatcatcaa gattatgtag ctaatattcg
    12901 atcagtttct agtatctaca tggtaaactt gcatgcactt gctacttatt tttgatatac
    12961 ttggatgata acatatgctg ctggttgatt cctacctaca tgatgaacat tttacaggcc
    13021 attagtgtct gtctgtatgt gttgttcctg tttgcttcag tctatttctg tttcattcct
    13081 agtttattgg ttctctgcta gatacttacc ctgctgggct tagttatcat cttatctcga
    13141 atgcattttc atgtttatag atgaatatac actcagatag gtgtagatgt atgctactgt
    13201 ttctctacgt tgctgtaggt tttacctgtg gcaactgcat actcctgttg cttcgctaga
    13261 tatgtatgtg cttatataga ttaagatatg tgtgatggtt ctttagtata tctgatgatc
    13321 atgtatgctc ttttaacttc ttgctacact tggtaacatg ctgtgatgct gtttgttgat
    13381 tctgtagcac taccaatgat gaccttatct ctctttgtat atgatgtttc tgtttgtttg
    13441 aggcttgtgt tactgctagt tacttaccct gttgcctggc taatcttctg cagatcctgc
    13501 aggttcactg ccgtataggc aggttttaga gctagaaata gcaagttaaa ataaggctag
    13561 tccgttatca acttgaaaaa gtggcaccga gtcggtgcgt tcactgccgt ataggcagaa
    13621 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
    13681 atgtataccc gaagtatgtc aaaaggaggt atgctatgaa gcagcgtatt acagtgacag
    13741 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
    13801 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
    13861 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
    13921 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
    13981 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
    14041 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
    14101 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtttccg
    14161 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
    14221 acctgatgtt ctggggaata taagctcttc agtcgatcga caagctcgag tttctccata
    14281 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
    14341 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
    14401 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattagagct
    14461 ctaccggtgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    14521 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    14581 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg
    14641 agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata
    14701 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
    14761 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
    14821 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
    14881 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
    14941 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
    15001 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
    15061 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
    15121 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
    15181 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
    15241 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
    15301 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
    15361 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
    15421 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
    15481 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
    15541 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
    15601 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
    15661 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
    15721 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
    15781 tcgcggccgg gtacgtgttc gagccgcccg cgcacggctc aaccgtgcgg ctgcatgaaa
    15841 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
    15901 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
    15961 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
    16021 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
    16081 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
    16141 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
    16201 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
    16261 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
    16321 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
    16381 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
    16441 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
    16501 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
    16561 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
    16621 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
    16681 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
    16741 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
    16801 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
    16861 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
    16921 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
    16981 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa
    17041 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
    17101 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
    17161 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
    17221 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
    17281 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
    17341 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
    17401 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
    17461 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
    17521 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
    17581 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
    17641 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
    17701 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
    17761 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
    17821 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
    17881 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
    17941 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
    18001 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
    18061 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
    18121 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
    18181 gcaggggaaa aaggtcgaaa aggcctcttt cctgtggata gcacgtacat tgggaaccca
    18241 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
    18301 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
    18361 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
    18421 cgcacagccc aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
    18481 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
    18541 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
    18601 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    18661 cccggaaacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    18721 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
    18781 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    18841 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gccctcttcc
    18901 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    18961 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    19021 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    19081 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    19141 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    19201 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    19261 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    19321 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    19381 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    19441 aggattagca gagcgaggta t
//
