LOCUS       sequence-182415-        2499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of wildtype Rag GTPase dimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Shen K, Choe A, Sabatini DM
  TITLE     Intersubunit Crosstalk in the Rag GTPase Heterodimer Enables mTORC1 
            to Respond Rapidly to Amino Acid Availability.
  JOURNAL   Mol Cell. 2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 
            10.1016/j.molcel.2017.09.026.
  PUBMED    29056322
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 
            10.1016/j.molcel.2017.09.026."
COMMENT     Sequence Label: pETDuet RagA-RagC
FEATURES             Location/Qualifiers
     source          1..2499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1028..1051
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     1120..1139
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1120..1138
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1139..1163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2418..2462
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atggcgccga acaccgctat gaaaaaaaaa gtgctgctga tgggtaaatc
      121 gggctcgggt aaaacctcaa tgcgtagtat tatctttgcg aactatattg cgcgtgatac
      181 ccgtcgcctg ggtgcaacga tcgacgtgga acatagccac gttcgctttc tgggtaacct
      241 ggtgctgaat ctgtgggatt gcggcggtca ggacaccttt atggaaaatt atttcacgtc
      301 gcaacgtgat aacatttttc gcaatgtcga agtgctgatc tacgttttcg atgtcgaatc
      361 ccgtgaactg gaaaaagaca tgcattatta ccagtcctgc ctggaagcaa ttctgcaaaa
      421 ctcaccggat gctaaaatct tttgtctggt ccacaaaatg gacctggtgc aggaagatca
      481 acgtgacctg attttcaaag aacgcgaaga agatctgcgt cgcctgtcgc gtccgctgga
      541 atgcgcgtgt tttcgcacca gcatctggga cgaaacgctg tataaagcct ggagctctat
      601 tgtttaccag ctgatcccga atgtccagca actggaaatg aacctgcgta atttcgcaca
      661 gattatcgaa gctgatgaag tgctgctgtt tgaacgcgca accttcctgg ttatttccca
      721 ttatcagtgc aaagaacaac gtgatgtgca ccgcttcgaa aaaatctcaa acatcatcaa
      781 acagttcaaa ctgagttgtt ccaaactggc ggcctcattt cagtcgatgg aagtccgcaa
      841 ctctaatttt gcagctttca ttgatatctt caccagtaat acgtacgtta tggtggttat
      901 gagcgacccg tctattccga gtgcggccac cctgattaac atccgtaatg cccgcaaaca
      961 ttttgaaaaa ctggaacgtg ttgatggccc gaaacacagc ctgctgatgc gcggtagcgg
     1021 tggcagccat caccatcatc atcaccatca ttaagcggcc gcataatgct taagtcgaac
     1081 agaaagtaat cgtattgtac acggccgcat aatcgaaatt aatacgactc actatagggg
     1141 aattgtgagc ggataacaat tccccatctt agtatattag ttaagtataa gaaggagata
     1201 tacatatgtc actgcaatac ggtgctgaag aaacgccgct ggctggtagt tatggtgctg
     1261 ctgattcatt cccgaaagat tttggttatg gcgttgaaga agaagaagaa gaagcggcgg
     1321 cggcgggcgg cggcgtgggt gcaggtgccg gtggcggttg tggcccgggc ggtgccgata
     1381 gctctaaacc gcgtattctg ctgatgggcc tgcgtcgcag tggtaaaagt tccatccaga
     1441 aagtggtttt tcacaaaatg tcgccgaacg aaaccctgtt cctggaaagc acgaacaaaa
     1501 tctacaaaga tgacatcagc aactcatcgt ttgtgaattt ccagatttgg gattttccgg
     1561 gtcaaatgga tttctttgac ccgacctttg actacgaaat gatcttccgt ggcacgggtg
     1621 cgctgattta tgttatcgat gcccaagatg actacatgga agcactgacc cgcctgcata
     1681 ttacggtgtc aaaagcgtat aaagttaacc cggatatgaa cttcgaagtt ttcatccata
     1741 aagtggatgg cctgtcggat gaccacaaaa ttgaaaccca gcgtgatatc catcaacgcg
     1801 caaacgatga cctggcagac gctggtctgg aaaaactgca cctgagtttt tatctgacca
     1861 gcatttacga tcattcaatc tttgaagcgt tcagcaaagt cgtgcagaaa ctgattccgc
     1921 aactgccgac gctggaaaac ctgctgaaca tcttcatcag taactccggc atcgaaaaag
     1981 cctttctgtt cgatgttgtc tctaaaatct acatcgcaac cgatagctct ccggtggaca
     2041 tgcagagtta cgaactgtgc tgtgatatga ttgacgtggt tatcgatgtt tcctgcattt
     2101 atggtctgaa agaagatggc tcaggttcgg cttacgacaa agaatcaatg gcgattatca
     2161 aactgaacaa taccacggtc ctgtatctga aagaagtgac caaatttctg gctctggtgt
     2221 gcattctgcg tgaagaatct ttcgaacgca aaggcctgat cgattacaac ttccattgtt
     2281 tccgtaaagc catccacgaa gtttttgaag tcggcgtgac gagccatcgc tcttgcggtc
     2341 accaaacgag cgcaagcagc ctgaaagccc tgacccacaa tggcaccccg cgtaatgcaa
     2401 tctgactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag cacatggact
     2461 cgtctactag cgcagcttaa ttaacctagg ctgctgcca
//
