LOCUS       sequence-18253-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Conze DB, Albert L, Ferrick DA, Goeddel DV, Yeh WC, Mak T, Ashwell 
            JD
  TITLE     Posttranscriptional downregulation of c-IAP2 by the ubiquitin 
            protein ligase c-IAP1 in vivo.
  JOURNAL   Mol Cell Biol. 2005 Apr . 25(8):3348-56.
  PUBMED    15798218
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Apr . 25(8):3348-56."
COMMENT     Sequence Label: pcmvtag2Bmciap2 mut
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             116..139
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctg gtttagtgac cgtcagatcc gctagcgatt acgccaagct cgaaattaac
       61 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgcca ccatggatta
      121 caaggatgac gacgataaga gcccgggcgg atccatgaac atggttcaag acagcgcctt
      181 tctagccaag ctgatgaaga gtgctgacac ctttgagttg aagtatgact tttcctgtga
      241 gctgtaccga ttgtccacgt attcagcttt tcccagggga gttcctgtgt cagaaaggag
      301 tctggctcgt gctggctttt actacactgg tgccaatgac aaggtcaagt gcttctgctg
      361 tggcctgatg ctagacaact ggaaacaagg ggacagtccc atggagaagc acagaaagtt
      421 gtaccccagc tgcaactttg tacagacttt gaatccagcc aacagtctgg aagctagtcc
      481 tcggccttct cttccttcca cggcgatgag caccatgcct ttgagctttg caagttctga
      541 gaatactggc tatttcagtg gctcttactc gagctttccc tcagaccctg tgaacttccg
      601 agcaaatcaa gattgtcctg ctttgagcac aagtccctac cactttgcaa tgaacacaga
      661 gaangccaga ttactcacct atgaaacatg gccattgtct tttctgtcac cagcaaagct
      721 ggccaaagca ggcttctact acataggacc tggagataga gtggcctgct ttgcgtgcga
      781 tgggaaactg agcaactggg aacg
//
