LOCUS       sequence-182597-        8630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Drosophila dati circular RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Liang D, Tatomer DC, Luo Z, Wu H, Yang L, Chen LL, Cherry S, Wilusz 
            JE
  TITLE     The Output of Protein-Coding Genes Shifts to Circular RNAs When the 
            Pre-mRNA Processing Machinery Is Limiting.
  JOURNAL   Mol Cell. 2017 Nov 9. pii: S1097-2765(17)30835-3. doi: 
            10.1016/j.molcel.2017.10.034.
  PUBMED    29174924
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2017 Nov 9. pii: S1097-2765(17)30835-3. doi: 
            10.1016/j.molcel.2017.10.034."
COMMENT     Sequence Label: Hy_pMT dati
FEATURES             Location/Qualifiers
     source          1..8630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        787..1066
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             1112..2137
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1112..2137
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          2500..2565
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2695..2715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3140..3274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3275..3701
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     polyA_signal    5209..5330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6040..6057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6211..6799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6291..6310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6970..7761,7762..7830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7593..7612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7831..7935)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8003..8021
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atctatgaaa gttaatataa gcaaacctta ctttgactca ataccaatgc actttgtgtc
       61 gataggttca cgcaattgag gcgattattc cgataaccca agcgattgac tgttcccgtt
      121 tcgattccaa ttgaaatttg gaaatgtaca atagttttgc tatatgctgt caagtacgct
      181 cttatcttct ctgggttttc ttcagagttt cgaaacgctt cttctttttt ttgttttttt
      241 ttttttggaa tctcgtattt tggaaggggc tcccctctgg aatttgttac actgtcgtta
      301 tcattgcgaa caagcggccc gaagctatca gcgactttaa catttacaat gcactttttt
      361 acgaccaatt aaatgtacat tttcctttct tcgcccgttg ataagcgaac gcgatgtggc
      421 gcaggcaatg tgttgctctt gcgacacaaa cgcaatcaaa atggattcaa tttcgctttt
      481 tcccagtgaa acgaagaacg aaccgaccat catgatatgc tcctctgcat gttgcgtatt
      541 gaatcaatga caatttcaat taagccgccc gttcgtcatg cgttttcgtg cgcttcgaaa
      601 tgctgataac gctgctgtcc tccaactgct ttgcatgtgg acacaattcc atttatttaa
      661 ttcttttatt tggatcggtt aaattaaaaa gcgccttgtt acgcatttaa cgttgtttcc
      721 ggtgcgtggt ggtttcatgc ttctgggaac ggcaaatggg tttaggattg ggaacccctc
      781 atcatctgtt ggaatatact attcaaccta caaaagtaac gttaaacaac actactttat
      841 atttgatatg aatggccaca ccttttatgc cataaaacat attgtaagag aataccactc
      901 tttttattcc ttctttcctt cttgtacgtt ttttgctgta agtaggtcgt ggtgctggtg
      961 ttgcagttga aataacttaa aatataaatc ataaaactca aacataaact tgactattta
     1021 tttatttatt aagaaaggaa atataaatta taaattacaa caggttatgg acctgcagcc
     1081 aagcttggcg ctcgtccggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
     1141 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     1201 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     1261 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     1321 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     1381 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     1441 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     1501 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     1561 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     1621 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     1681 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     1741 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     1801 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     1861 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     1921 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     1981 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     2041 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     2101 ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgaacaagag
     2161 ctgatttcga gaacgcctca gccagcaact cgcgcgagcc tagcaaggca aatgcgagag
     2221 aacggcctta cgcttggtgg cacagttctc gtccacagtt cgctaagctc gctcggctgg
     2281 gtcgcgggag ggccggtcgc agtgattcag gcccttctgg attgtgttgg tccccagggc
     2341 acgattgtca tgcccacgca ctcgggtgat ctgactgatc ccgcagattg gagatcgccg
     2401 cccgtgcctg ccgattgggt gcagatcttt gtgaaggaac cttacttctg tggtgtgaca
     2461 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     2521 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     2581 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     2641 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     2701 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     2761 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     2821 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     2881 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     2941 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     3001 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     3061 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3121 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3181 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3241 caaactcatc aatgtatctt atcatgtctg gatcaattcg ttgcaggaca ggatgtggtg
     3301 cccgatgtga ctagctcttt gctgcaggcc gtcctatcct ctggttccga taagagaccc
     3361 agaactccgg ccccccaccg cccaccgcca cccccataca tatgtggtac gcaagtaaga
     3421 gtgcctgcgc atgccccatg tgccccacca agagctttgc atcccataca agtccccaaa
     3481 gtggagaacc gaaccaattc ttcgcgggca gaacaaaagc ttctgcacac gtctccactc
     3541 gaatttggag ccggccggcg tgtgcaaaag aggtgaatcg aacgaaagac ccgtgtgtaa
     3601 agccgcgttt ccaaaatgta taaaaccgag agcatctggc caatgtgcat cagttgtggt
     3661 cagcagcaaa atcaagtgaa tcatctcagt gcaactaaag gggggatctc gagttcaatc
     3721 tatatgccga aaacatattg accacgccca ctctgacccc taaaaccgcc caattttaaa
     3781 aattcaaaaa acccttttac gcttaacaac tttttttgtt ttacaggcaa taaaatgata
     3841 tgaaattacg atgctttaac caggcccaaa cttcgcacag ttggcacccc agatccgtgt
     3901 tcagggtcgt aagcagttgt cctcccgatc gaaaaaaacg gccaagccca gcgagctggc
     3961 aaatctgtat tggccatctc ctaacaaatt atcttggaat gtttaaaatg gactccgcag
     4021 acttctggca gcaagcgcgt gctccgtttg gtctgcaaac tgcgcttcac caatactctt
     4081 cccccccgaa tcaacagcca atatcccacc acgccctgca ccactccgtt ccgcaggaga
     4141 acgagcagtt ggccggagtt caaccagaag gtccaacagc aggatcaggc gtagtgggca
     4201 acaacagttc catggcagtt tccggcacca attcaactgt gggcagcaag gcggagtcca
     4261 gtcacattcc gcagcagcac tcagagcagc agtcatatgg aagcgactcc ttccgaggaa
     4321 cgcagtcgcc ccagctgagt agccaccacc tgttgtttaa cgccgctgca gccgctgctg
     4381 cggcggtcca cctcaagtcc acggcgatgc agaataacct ctcgccaata ggcgatcagg
     4441 tgcaaaataa ccttcgcaac tacggacaag gatccttaaa cgccttatgt gggatcaagc
     4501 ccaagcagga gatggatgcg aagacgcccc tacagccgtt ggacgagtgt ccccaccctc
     4561 tagttcaggc gcaggcccgg tcccagtacg gcggagacta ctacgacgtg gccgacccac
     4621 aggctagaga aatctcggaa ggtcgagcac tgataatcgg actcggagca cccagtacgg
     4681 tgtccaccga cgatgcacag tccagtgcgc cgtctcatca gctggcaggg actggccgag
     4741 cattgcagac tcatcagtgc aagccaatgt cccctggaac agttggtagc tcgaatttgg
     4801 gcgcaggacg aagatccgca cccacaacaa taagcaagac cttttcgcag ggtcagcagt
     4861 cccctcagca ctctacaacg ccatccggag gcagcacgac gcccgacatc aaatacaata
     4921 acgacaaaat ggccaatgaa attcaggtat tgttttgaag atttttctgc ttatctatat
     4981 actttaaagg gaacctggta atcgaatatt cgatgcagtc caaacaagag tagtaaaaaa
     5041 tcaacagaga acgctattcg agtttttaga ctagactata tgtacacccg atatttagct
     5101 gttacgagcg caacaatgaa ctttcaaaat tgtgtgactg ttttggacgt tttgcaggtg
     5161 taagtagggg cgtggccaaa gtgtttttgg tatgtcaata gcggccgcaa cttgtttatt
     5221 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5281 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta actagtggat
     5341 ccactagggg ccgccgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     5401 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     5461 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc
     5521 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     5581 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     5641 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     5701 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     5761 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     5821 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     5881 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     5941 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     6001 taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg
     6061 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6901 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6961 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7021 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7081 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7141 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7201 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7261 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     7321 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7381 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7441 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7501 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7561 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     7621 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7681 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7741 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7801 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7861 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7921 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     7981 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
     8041 cacatttgta cgaatttttt ttttatcaaa agttcgagtt tttcaccaat ttcctcatca
     8101 accgagcaag gcaaacggct ttgaataata tggtgttata tatacatata tcaaatcgct
     8161 gctgactgcg tgattgatgg ccccaagatt acatattatc gaatcaggat tcagaaggag
     8221 atcaatgtca aatgcggaca ggaacatgaa agacgcctgt tatgcgcaat taaaaatttg
     8281 ggtttaattg ctgtggaaac tgttgttggc ggcatcttaa gttcctgttt aacaacatca
     8341 actacttatg tacgtagaag cgtttaagcc atttgcatac agatgagaac tggcttttgt
     8401 gctaatcagt caagatgact ccgatgatga tgactcatta cctgaccagt tttcgctgct
     8461 ttcttttcaa caactacttg tatatgtatt gtatccaata gcaatacatt gaatttccat
     8521 ggtctagtca cgtattatca tttaattgac accaagtcgt gttattgttg agctatcgag
     8581 ttcagctcaa acatttctta ttcccatgaa taagccggca aaaatatgca
//
