LOCUS       sequence-182779-       13026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancing the lower-part mevalonate pathway; overexpressing yeast 
            mevalonate pathway genes under the control of consitutive promoters 
            or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13026)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 13026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pIMVAd39T
FEATURES             Location/Qualifiers
     source          1..13026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1654..1665)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(1947..2650)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     promoter        complement(3883..4296)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        4466..4747
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             4748..5422
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(5898..5914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5898..5914)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5911..5933)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5922..5938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5946..5952,5953..5970,5971..5976))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5991..6012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6129..6146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6300..6888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6380..6399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7059..7850,7851..7919))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7682..7701
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7920..8024)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8092..8110
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8148..8170)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8270..8289
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8483..8505
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8497..8514
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8498..8514
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgttgatct tagggctcaa accgctgatg acaaaagatt ttctaaggtt caatggctgg
       61 atgtaactca ggctgactgg ggtgttagga aagaaaaaga tccggaaact tatcttgata
      121 aataatagtg atcgaattta cgtagcccaa tctaccactt ttttttttca ttttttaaag
      181 tgttatactt agttatgctc taggataatg aactactttt tttttttttt ttttactgtt
      241 atcataaata tatatacctt attgttgttt gcaaccgtcg gttaattcct tatcaaggtt
      301 ccccaagttc ggatcattac catcaatttc caacattttc atgagttctt cttcttcatt
      361 accgtgtttt agggggctgt tcgcacttct aatagggcta tcaccaagct gttctaattc
      421 gtccaaaagt tactagttgt ttcaattacg caggaccctt ttacgattgt ttttcatctc
      481 tagaggcatt ttcaacatcg tattttccga agcgttgcat aatgaaaatg tgataataag
      541 gcagtgagct actttgtcac aacaaagtac gagaacagat gaggttccgt ggtgtttttg
      601 gggcaaccgc actatttgga gcgctgtata tatttatatc ctactacaaa aaatcataaa
      661 tgacactgaa ttggacacat gctagcgtca aataccttgc ctggtaaagt tgtgtgctag
      721 tgtctcccgt cttctgttca ctattattcc tttggtagac cagtctttgc gtcaatcaaa
      781 gattcgtttg tttcttgtgg gcctgaaccg acttgagtta aaatcactct ggcaacatcc
      841 ttttgcaact caagatccaa ttcacgtgca gtaaagttag atgattcaaa ttgatggttg
      901 aaagcctcaa gctgctcagt agtaaatttc ttgtcccatc caggaacaga gccaaacaat
      961 ttatagataa atgcaaagag tttcgactca ttttcagcta agtagtacaa cacagcattt
     1021 ggacctgcat caaacgtgta tgcaacgatt gtttctccgt aaaactgatt aatggtgtgg
     1081 caccaactga tgatacgctt ggaagtgtca ttcatgtaga atattggagg gaaagagtcc
     1141 aaacatgtgg catggaaaga gttggaatcc atcattgttt cctttgcaaa ggtggcgaaa
     1201 tctttttcaa caatggcttt acgcatgact tcaaatctct ttggtacgac atgttcaatt
     1261 ctttctttaa atagttcgga ggttgccacg gtcaattgca taccctgagt ggaactcaca
     1321 tcctttttaa tatcgctgac aactaggaca caagctttca tctgaggcca gtcagagctg
     1381 tctgcgattt gtactgccat ggaatcatga ccatcttcag cttttcccat ttcccaggcc
     1441 acgtatccgc caaacaacga tctacaagct gaaccagacc cctttcttgc tattctagat
     1501 atttctgaag ttgactgtgg taattggtat aacttagcaa ttgcagagac caatgcagca
     1561 aagccagcag cggaggaagc taaaccagct gctgtaggaa agttattttc ggagacaatg
     1621 tggagtttcc attgagataa tgtgggcaat gaggcgtcct tcgattccat ttcctttctt
     1681 aattggcgta ggtcgcgcag acaattttga gttctttcat tgtcgatgct gtgtggttct
     1741 ccatttaacc acaaagtgtc gcgttcaaac tcaggtgcag tagccgcaga ggtcaacgtt
     1801 ctgaggtcat cttgcgataa agtcactgat atggacgaat tggtgggcag attcaacttc
     1861 gtgtcccttt tcccccaata cttaagggtt gcgatgttga cgggtgcggt aacggatgct
     1921 gtgtaaacgg tcattgtata tgagatagtt gattgtatgc ttggtatagc ttgaaatatt
     1981 gtgcagaaaa agaaacaagg aagaaaggga acgagaacaa tgacgaggaa acaaaagatt
     2041 aataattgca ggtctattta tacttgatag caagacagca aacttttttt tatttcaaat
     2101 tcaagtaact ggaaggaagg ccgtataccg ttgctcatta gagagtagtg tgcgtgaatg
     2161 aaggaaggaa aaagtttcgt gtgcttcgag atacccctca tcagctctgg aacaacgaca
     2221 tctgttggtg ctgtctttgt cgttaatttt ttcctttagt gtcttccatc attttttgtc
     2281 attgcggata tggtgagaca acaacggggg agagagaaaa gaaaaaaaaa gaaaagaagt
     2341 tgcatgcgcc tattattact tcaatagatg gcaaatggaa aaagggtagt gaaacttcga
     2401 tatgatgatg gctatcaagt ctagggctac agtattagtt cgttatgtac caccatcaat
     2461 gaggcagtgt aattggtgta gtcttgttta gcccattatg tcttgtctgg tatctgttct
     2521 attgtatatc tcccctccgc cacctacatg ttagggagac caacgaaggt attataggaa
     2581 tcccgatgta tgggtttggt tgccagaaaa gaggaagtcc atattgtaca cccggaaaca
     2641 acaaaaggat atccgaaata ttccacggtt tagaaaaaaa tcggaaaaga gcgtcgactg
     2701 gtagtgccac cacctatgta agctgctagc attggtatac gtagaacaac taggactccc
     2761 tcgtccgctc cttgatacag gtagataagt tctgcctgag gggaggattg agaagagggg
     2821 gtaggggttt gcttgatagc agaataaaag tacagctcta ggtctataaa aaaaaattgg
     2881 ttaaagaaaa aatatacagt tttgtatata gaatcattaa ttaaaaagat aatagtataa
     2941 aacacatcag ataaacaaaa gtttgcccaa taaaattata ttttccatca accagctcac
     3001 tattatagca ttctatgaat ttgcctgtca ttttccactt cagaaaggtc atctaattgc
     3061 tcccaccagt tgaataagta attctcgcaa ataatcttaa accaaggcgt aaacttgtaa
     3121 cttgggtcag caaacatagt tttcaaatca tttggtgaaa cccatttgaa gtctctaact
     3181 tcattgacgt ttgggttgac agtcaagttt tctttagcgt tgatcttata aaataggatg
     3241 taatcaattt catgttcacc ccatggttca ttgcttggtg ccatgtaatg gattctgttt
     3301 aaaaagtgaa acttacccct tgtcttagtt tcatcttctg gaatacctaa ttcatgatct
     3361 agttttctca ccgccgcagt aatagcgccc ttaatcttat cgtctagctt acccttcaaa
     3421 cctaattcgt catcaataca tagtggatga gagcagcatg tgttagtcca aagatcaggg
     3481 aaagttattt tttcagtggc tctttgttgt aaaagtaatt caccttgttc attgaaaata
     3541 aagacggaga atgcacgatg tagtaaaccc ttttcaatat tttccattaa atgacaaact
     3601 ttcttggtac cggcaccaat agcattatcg tcccaatcca aaacaataca attttcattc
     3661 attaacttaa tttgctcctc atcatgacca gaaaaacatg tttctccgct ttcgtcattt
     3721 gacgtctcac tagatcgggt attaggtctt tgttgtaatg gaataatttc aggaaactct
     3781 tccaaaatgt cttcaggtgt ttggttttgc actaatttgg cgtaactaga tactgcacca
     3841 tggggcatac tattgttgtc ggcagtcatt ttgtaattaa aacttagatt agattgctat
     3901 gctttctttc taatgagcaa gaagtaaaaa aagttgtaat agaacaagaa aaatgaaact
     3961 gaaacttgag aaattgaaga ccgtttatta acttaaatat caatgggagg tcatcgaaag
     4021 agaaaaaaat caaaaaaaaa aattttcaag aaaaagaaac gtgataaaaa tttttattgc
     4081 ctttttcgac gaagaaaaag aaacgaggcg gtctcttttt tcttttccaa acctttagta
     4141 cgggtaatta acgacaccct agaggaagaa agaggggaaa tttagtatgc tgtgcttggg
     4201 tgttttgaag tggtacggcg atgcgcggag tccgagaaaa tctggaagag taaaaaagga
     4261 gtagaaacat tttgaagcta tggtgtgtgg gggatcactt gtgggggatt gggtgtgatg
     4321 taaggattcg cggtcctcga aaattaaaag tccaacgcgc ctgttgcttc ctatgtgata
     4381 tgtattatat gtaatatgca taaatatatc tactgcattg tattttgaac gtacaaagta
     4441 tgcattgttt atacgcggcc gcgttaacga cattactata tatataatat aggaagcatt
     4501 taatagaaca gcatcgtaat atatgtgtac tttgcagtta tgacgccaga tggcagtagt
     4561 ggaagatatt ctttattgaa aaatagcttg tcaccttacg tacaatcttg atccggagct
     4621 tttctttttt tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag agggccaaga
     4681 gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca caaaggcagc
     4741 ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga aagtttgcgg
     4801 cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact tgctgggtat
     4861 tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga aaatttcaag
     4921 tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg gcgtgtttcg
     4981 taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg atatcgtcca
     5041 actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc cagttattaa
     5101 aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac agaaacctca
     5161 ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt tggattggaa
     5221 ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt ttatgttagc
     5281 tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg ttattggtgt
     5341 tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt
     5401 cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt attgtttgtg
     5461 cacttgccga tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     5521 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     5581 tcatttaaat ctcttttgga taagctttat gaagtcaaag gtatgagatg ggctggtaac
     5641 gctaacgaat tgaacgctgc ctatgctgct gatggttacg ctcgtatcaa gggtatgtcc
     5701 tgtattatta ccaccttcgg tgttggtgaa ttgtctgctt tgaatggtat tgccggttct
     5761 tacgctgaac atgtcggtgt tttgcacgtt gttggtgttc catccatctc ttctcaagct
     5821 aagcaattgt tgttgcatca taccttgggt aacggtgact tcactgttta aacgcatgca
     5881 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5941 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6001 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6061 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6121 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6181 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6241 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6301 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6361 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6421 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6481 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6541 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6601 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6661 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6721 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6781 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6841 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6901 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6961 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7021 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7081 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7141 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7201 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7261 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7321 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7381 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7441 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7501 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7561 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7621 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7681 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7741 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7801 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7861 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7921 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7981 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8041 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     8101 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     8161 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     8221 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     8281 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     8341 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     8401 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     8461 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
     8521 gtttaaacta atacgtaaac ctgcattaag gtaagattat atcagaaaat gtgttgcaag
     8581 aaatgcatta tgcaattttt tgattatgac aatctctcga aagaaatttc atatgatgag
     8641 acttgaataa tgcagcggcg cttgctaaaa gaacttgtat ataagagctg ccattctcga
     8701 tcaatatact gtagtaagtc ctttcctctc tttcttatta cacttatttc acataatcaa
     8761 tctcaaagag cctgcaggtg tactaatcca aggaggttta cggaccagag gaactttcca
     8821 gattcagatc acagcaatat aggactggaa aacatcaggt agccgcactc aacttgtaac
     8881 tggcaactac tttgcattaa actccaatta aatgcggtag aatcttttca gaaaaggtat
     8941 tcaacgtcaa ttcggaaagc ttccttccgg aatggcttaa gtaggttgca atttcttttt
     9001 ctattagtag ctaaaaatgg gtcacgtgat ctatattcga aaggggcggt tgcctcagga
     9061 aggcaccggc ggtctttcgt ccgtgcggag atatctgcgc cgttcagggg tccatgtgcc
     9121 ttggacgata ttaaggcaga aggcagtatc ggggcggatc actccgaacc gagattagtt
     9181 aagcccttcc catctcaaga tggggagcaa atggcattat actcctgcta gaaagttaac
     9241 tgtgcacata ttcttaaatt atacaatgtt ctggagagct attgtttaaa aaacaaacat
     9301 ttcgcaggct aaaatgtgga gataggatta gttttgtaga catatataaa caatcagtaa
     9361 ttggattgaa aatttggtgt tgtgaattgc tcttcattat gcaccttatt caattatcat
     9421 caagaatagc aatagttaag taaacacaag attaacataa taaaaaaaat aattctttca
     9481 taatgtcatt accgttctta acttctgcac cgggaaaggt tattattttt ggtgaacact
     9541 ctgctgtgta caacaagcct gccgtcgctg ctagtgtgtc tgcgttgaga acctacctgc
     9601 taataagcga gtcatctgca ccagatacta ttgaattgga cttcccggac attagcttta
     9661 atcataagtg gtccatcaat gatttcaatg ccatcaccga ggatcaagta aactcccaaa
     9721 aattggccaa ggctcaacaa gccaccgatg gcttgtctca ggaactcgtt agtcttttgg
     9781 atccgttgtt agctcaacta tccgaatcct tccactacca tgcagcgttt tgtttcctgt
     9841 atatgtttgt ttgcctatgc ccccatgcca agaatattaa gttttcttta aagtctactt
     9901 tacccatcgg tgctgggttg ggctcaagcg cctctatttc tgtatcactg gccttagcta
     9961 tggcctactt gggggggtta ataggatcta atgacttgga aaagctgtca gaaaacgata
    10021 agcatatagt gaatcaatgg gccttcatag gtgaaaagtg tattcacggt accccttcag
    10081 gaatagataa cgctgtggcc acttatggta atgccctgct atttgaaaaa gactcacata
    10141 atggaacaat aaacacaaac aattttaagt tcttagatga tttcccagcc attccaatga
    10201 tcctaaccta tactagaatt ccaaggtcta caaaagatct tgttgctcgc gttcgtgtgt
    10261 tggtcaccga gaaatttcct gaagttatga agccaattct agatgccatg ggtgaatgtg
    10321 ccctacaagg cttagagatc atgactaagt taagtaaatg taaaggcacc gatgacgagg
    10381 ctgtagaaac taataatgaa ctgtatgaac aactattgga attgataaga ataaatcatg
    10441 gactgcttgt ctcaatcggt gtttctcatc ctggattaga acttattaaa aatctgagcg
    10501 atgatttgag aattggctcc acaaaactta ccggtgctgg tggcggcggt tgctctttga
    10561 ctttgttacg aagagacatt actcaagagc aaattgacag tttcaaaaag aaattgcaag
    10621 atgattttag ttacgagaca tttgaaacag acttgggtgg gactggctgc tgtttgttaa
    10681 gcgcaaaaaa tttgaataaa gatcttaaaa tcaaatccct agtattccaa ttatttgaaa
    10741 ataaaactac cacaaagcaa caaattgacg atctattatt gccaggaaac acgaatttac
    10801 catggacttc ataatagtga cacatttctt aacagatggc tgaaaaattt tttttttttt
    10861 tttggttttg ctttgtatac acatgtatct atttttataa agatgaaata tatacgtcta
    10921 agagctaaaa tgaaaaacta tactaatcac ttatatctat tctggtgatt catcatcttc
    10981 agcttctgta tccgtatcga tatcagtata atcgctagca tcactttcat catcactttc
    11041 ggtttcgtcg tcatcagtag agtactcatc atcctcctca gaatacacct ctacttgttc
    11101 cactgcagga gaatcacgaa gagctcactt tgttatgtag agttttttta gctacctata
    11161 ttccaccata acatcaatca tgcggttgct ggtgtattta ccaataatgt ttaatgtata
    11221 tatatatata tatatggggc cgtatactta catatagtag atgtcaagcg taggcgcttc
    11281 ccctgccggc tgtgagggcg ccataaccaa ggtatctata gaccgccaat cagcaaacta
    11341 cctccgtaca ttcatgttgc acccacacat ttatacaccc agaccgcgac aaattaccca
    11401 taaggttgtt tgtgacggcg tcgtacaaga gaacgtggga actttttagg ctcaccaaaa
    11461 aagaaagaaa aaatacgagt tgctgacaga agcctcaaga aaaaaaaaat tcttcttcga
    11521 ctatgctgga ggcagagatg atcgagccgg tagttaacta tatatagcta aattggttcc
    11581 atcaccttct tttctggtgt cgctccttct agtgctattt ctggcttttc ctattttttt
    11641 ttttccattt ttctttctct ctttctaata tataaattct cttgcatttt ctatttttct
    11701 ctctatctat tctacttgtt tattcccttc aaggtttttt tttaaggagt acttgttttt
    11761 agaatatacg gtcaacgaac tataattaac taaacatgtc agagttgaga gccttcagtg
    11821 ccccagggaa agcgttacta gctggtggat atttagtttt agatccgaaa tatgaagcat
    11881 ttgtagtcgg attatcggca agaatgcatg ctgtagccca tccttacggt tcattgcaag
    11941 agtctgataa gtttgaagtg cgtgtgaaaa gtaaacaatt taaagatggg gagtggctgt
    12001 accatataag tcctaaaact ggcttcattc ctgtttcgat aggcggatct aagaaccctt
    12061 tcattgaaaa agttatcgct aacgtattta gctactttaa gcctaacatg gacgactact
    12121 gcaatagaaa cttgttcgtt attgatattt tctctgatga tgcctaccat tctcaggagg
    12181 acagcgttac cgaacatcgt ggcaacagaa gattgagttt tcattcgcac agaattgaag
    12241 aagttcccaa aacagggctg ggctcctcgg caggtttagt cacagtttta actacagctt
    12301 tggcctcctt ttttgtatcg gacctggaaa ataatgtaga caaatataga gaagttattc
    12361 ataatttatc acaagttgct cattgtcaag ctcagggtaa aattggaagc gggtttgatg
    12421 tagcggcggc agcatatgga tctatcagat atagaagatt cccacccgca ttaatctcta
    12481 atttgccaga tattggaagt gctacttacg gcagtaaact ggcgcatttg gttaatgaag
    12541 aagactggaa tataacgatt aaaagtaacc atttaccttc gggattaact ttatggatgg
    12601 gcgatattaa gaatggttca gaaacagtaa aactggtcca gaaggtaaaa aattggtatg
    12661 attcgcatat gccggaaagc ttgaaaatat atacagaact cgatcatgca aattctagat
    12721 ttatggatgg actatctaaa ctagatcgct tacacgagac tcatgacgat tacagcgatc
    12781 agatatttga gtctcttgag aggaatgact gtacctgtca aaagtatcct gagatcacag
    12841 aagttagaga tgcagttgcc acaattagac gttcctttag aaaaataact aaagaatctg
    12901 gtgccgatat cgaacctccc gtacaaacta gcttattgga tgattgccag accttaaaag
    12961 gagttcttac ttgcttaata cctggtgctg gtggttatga cgccattgca gtgattgcta
    13021 agcaag
//
