LOCUS       sequence-182796-        6446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, 
            Dokholyan NV, Hahn KM
  TITLE     Engineering extrinsic disorder to control protein activity in living
            cells.
  JOURNAL   Science. 2016 Dec 16;354(6318):1441-1444.
  PUBMED    27980211
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-12-16"; volume: "354(6318)"; pages: "1441-1444"
COMMENT     Sequence Label: mCherry-uniRapR-P-Vav2(DPZ)
FEATURES             Location/Qualifiers
     source          1..6446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..361
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4..195
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(27..47)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      212..347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             396..1190
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             396..1190
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(450..469)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1060..1079
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1378..1397)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1422..1469
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1798..2386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2287..2306
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2561..2864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2865..3068
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3019..3039
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3100..3807
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3245..3263)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3513..3532
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5734..5855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5771..5790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5825..5844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5862..6317)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5999..6020)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6211..6230
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(6344..6446,1..2)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
       61 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
      121 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      181 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
      241 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
      301 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
      361 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
      421 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
      481 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
      541 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
      601 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
      661 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
      721 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
      781 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
      841 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
      901 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
      961 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     1021 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     1081 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     1141 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     1201 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     1261 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     1321 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     1381 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     1441 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     1501 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     1561 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     1621 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     1681 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1741 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1801 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1861 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1921 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1981 gaactctgta tcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2041 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2101 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2161 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2221 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2281 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2341 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2401 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2461 atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa
     2521 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2581 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2641 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2701 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2761 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2821 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2881 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2941 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     3001 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     3061 gcagagctgg tttagtgaac cgtcagatcc gctagcacca tggtgagcaa gggcgaggag
     3121 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     3181 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3241 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     3301 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     3361 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3421 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     3481 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     3541 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     3601 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     3661 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     3721 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     3781 accggcggca tggacgagct gtacaagtcc ggaatcaagg tggaggtgca gcagcccatg
     3841 aaaatgggca tgactgagga cgacaagaga agctgctgct tgttagagat tcaggagacc
     3901 gaggccaagt actaccgcac cctggaggac attgagaaga actacatggg tcccttgcgg
     3961 ctggtgctga gcccggcgga tatggctgct gtcttcatca acctggagga cctcatcaag
     4021 gtgcatcaca gctttctgcg agccatcgat gtgtccatga tggctggtgg cagtaccctg
     4081 gctaaggtct ttctggagtt taaggaaagg ctcctgatct atggagagta ctgtagccac
     4141 atggaacacg ctcagagtac actgaaccag ctcctcgcca gccgagagga cttcaggcag
     4201 aaagtggagg agtgcacact cagggttcag gatggcaagt tcaagctgca agacctgctg
     4261 gtggtgccca tgcaacgggt gctgaagtac cacctgctgc tcaaggagct cctgagccat
     4321 tctgcaccaa cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
     4381 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
     4441 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
     4501 ccagattatg cctatggtgc cactgggcac ggttcgggct ccggatcagg cgtcaaggac
     4561 ctcctccaag cctgggacct ctattatcat gtgttccgac gaatctcagg tcctccagga
     4621 cctggatcag gtctctggca tgagatgtgg catgaaggcc tggaagaggc atctcgtttg
     4681 tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc tggagccctt gcatgctatg
     4741 atggaacggg gcccccagac tctgaaggaa acatccttta atcaggccta tggtcgagat
     4801 ttaatggagg cccaagagtg gtgcaggaag tacatgaaat cagggtcatc agggggctcc
     4861 ggatcaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg
     4921 gaacccgacc gaccagaaag acaacagctc aaagaagccc tggaagccat gcaggacttg
     4981 gccatgtaca tcaatgaagt gaagcgggac aaggagacct tgaagaagat tagcgagttc
     5041 cagtgctcca tagaaaacct gcaagtgaag ctggaggaat ttgggaggcc aaagattgac
     5101 ggggagctta aagtccggtc catagtcaac cacaccaagc aagacaggta cctgttccta
     5161 tttgacaagg tggtcatcgt gtgcaagagg aagggctaca gctatgagct gaaggaggtc
     5221 attgagctgc tcttccacaa gatgaccgat gacccgatgc acaacaagga catcaagaag
     5281 tggtcctatg gcttctacct gattcacctc caaggaaagc aaggctttca gttcttctgc
     5341 aagacggaag acatgaagcg gaagtggatg gagcagttcg agatggccat gtcaaacatc
     5401 aagccagata aggccaatgc caaccatcat agcttccaga tgtacacatt cgacaagact
     5461 accaactgca aagcctgcaa gatgtttctc aggggtacct tctaccaggg atacctgtgt
     5521 accagatgtg gcgtcggggc acacaaggaa tgcctggagg tgatcccccc ctgcaagatg
     5581 agttcacctg cagatgtgga tgctcctgga gcataatcta gataactgat cataatcagc
     5641 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     5701 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     5761 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5821 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat
     5881 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     5941 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     6001 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     6061 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     6121 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     6181 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     6241 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     6301 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6361 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6421 gcttcaataa tattgaaaaa ggaaga
//
