LOCUS       sequence-182829-        9802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A Cas9-encoding plasmid containing the 5' portion of the bsdMX 
            marker and the rrk1 promoter/leader. When linearized by NotI, it 
            serves as the gapped vector in the split-bsdMX system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Zhang XR, He JB, Wang YZ, Du LL
  TITLE     A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in 
            Fission Yeast.
  JOURNAL   G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 
            10.1534/g3.118.200164.
  PUBMED    29703785
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 
            10.1534/g3.118.200164."
COMMENT     Sequence Label: pDB4281
FEATURES             Location/Qualifiers
     source          1..9802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..742
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             763..4866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             763..4866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             763..4866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4879..4899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4915..5162
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4931..4949)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     5209..5227
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5265..5287)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5387..5406
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      5777..6558
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        6636..6979
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(7255..7611)
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     primer_bind     complement(7648..7664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7648..7664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7661..7683)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7672..7688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7696..7702,7703..7720,7721..7726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7741..7762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7879..7896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8050..8638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8130..8149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8809..9600,9601..9669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9432..9451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9670..9774)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccctacaac aactaagaaa atggctatca tgcggaagct ggtgagaaga acagcatcgg
       61 gacaagggaa ggaagaacaa agacaaagaa aacaaaagaa agcaattgaa aacaaaacaa
      121 aacaattttc attccttctc ttatcattcc ttttcttttc ttttctctca ttcaacgcac
      181 tccatcgtat ccgtattcct cttatttttt ctctttctct atatccattt ctttctctct
      241 aggtgtgtcc tctctctctc ttcaatttct ctactccgca ttccaacgca tccttccccc
      301 aacctcccat ttcctcctta cggcccgata gcgatcgtct ttccctcgct atcactcgct
      361 accggcccct cctctgcacc gtaacctcct acgtatttac catatcataa agttttttcc
      421 gacgcttatc gctgaccccc tgtcgccctc ctattggctt ccggattatc ttcttgtcca
      481 taaggtgatc catgcttcct gaagattccc gaaatgtgtc cactttggcg gggaatcatt
      541 ccatccactt ctttctctct cgctttcctc attcggcgct ccccttccgc gtctcattgg
      601 tcttccgctc cgtttttgct ttgccgatgt tacttgggga gaggtgcgat aatcctttcg
      661 caaaaactcg gtttgacgcc tcccatggta taaatagtgg gtggtggaca ggtgccttcg
      721 cttttcttta agcaagagaa ttgctgcagg tcgactctag agatggacaa gaagtactcc
      781 attgggctcg atatcggcac aaacagcgtc ggttgggccg tcattacgga cgagtacaag
      841 gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac
      901 ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga
      961 acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt
     1021 agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg
     1081 gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg
     1141 gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact
     1201 gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga
     1261 cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc
     1321 caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt
     1381 gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc
     1441 gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc
     1501 gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg
     1561 agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg cgaccagtac
     1621 gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag tgatattctg
     1681 cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa gcgctatgat
     1741 gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact gcctgagaag
     1801 tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat tgacggcgga
     1861 gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat ggacggcacc
     1921 gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg cactttcgac
     1981 aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct caggcggcaa
     2041 gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat cctcacattt
     2101 cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc gtggatgact
     2161 cgcaaatcag aagagaccat cactccctgg aacttcgagg aagtcgtgga taagggggcc
     2221 tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc taacgaaaag
     2281 gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga gctcaccaag
     2341 gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga gcagaagaaa
     2401 gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa
     2461 gactatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc
     2521 ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga caaggacttc
     2581 ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct tacgttgttt
     2641 gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt cgacgacaaa
     2701 gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc aagaaaactg
     2761 atcaatggga tccgagataa gcagtctgga aagacaatcc tggattttct taagtccgat
     2821 ggatttgcca accggaactt catgcagttg atccatgatg actctctcac ctttaaggag
     2881 gacatccaga aagcacaagt ttctggccag ggggacagtc ttcacgagca catcgctaat
     2941 cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt cgtggatgaa
     3001 ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat ggcccgagag
     3061 aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag gattgaagag
     3121 ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa cacccagctt
     3181 cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta cgtggatcag
     3241 gaactggaca tcaatcggct ctccgactac gacgtggatc atatcgtgcc ccagtctttt
     3301 ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa tagagggaag
     3361 agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg gcggcagctg
     3421 ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc tgaacgaggt
     3481 ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga gacacgccag
     3541 atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta cgatgaaaat
     3601 gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt ctcagatttc
     3661 agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca tgcgcatgat
     3721 gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa gcttgaatct
     3781 gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc aaagtctgag
     3841 caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat gaattttttc
     3901 aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat cgaaacaaac
     3961 ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc
     4021 ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg aggcttctcc
     4081 aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa aaaagattgg
     4141 gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt actggttgtg
     4201 gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc
     4261 acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga ggcgaaagga
     4321 tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct ctttgagctt
     4381 gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg taacgagctg
     4441 gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga aaagctcaaa
     4501 gggtctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa acactacctt
     4561 gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc cgacgctaac
     4621 ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag ggagcaggca
     4681 gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc cttcaagtac
     4741 ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct ggacgccaca
     4801 ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc tcagctcggt
     4861 ggagacagca gggctgaccc caagaagaag aggaaggtgt gatctcttct cgagtcatgt
     4921 aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg
     4981 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat
     5041 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat
     5101 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt
     5161 gcgatccccg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     5401 attgtactga gagtgcacca taccatcgat ggccaacctt ccaattcatt aaatcgagtc
     5461 ctccatcaga tgatttacaa ggttttgcat agaatcccta agtaagagaa gttgttagtg
     5521 atttcttcaa gtttactaaa atttacatac aaatggatga tctcttctcc attgtttcct
     5581 ttcttcttgc aaacgcgttt tacaaagaga tgacatgcta ttttctgatt atttttttct
     5641 atagttttct attttgtacg tccttgggag tccgaaatgt aaaatcgggg tatggttagt
     5701 agcggcagcg ttcattatgg tatataaaag ataaaaagta ggggcaagcg agacagaatg
     5761 ggatacaagg gcatcgtcta taattatagc taaaaattgt attttaattt gtattttttg
     5821 taattttatt tctcattgtt ttacttaaaa tgaatgcgaa ttagagaaaa cttcaacgaa
     5881 atgtcaaaat aagctcagca aaatatacaa ttttagggaa agcgatcagc aacttattct
     5941 ccgttgtaga ttctttttct tcataaattc caacaatgta actgccaatt gatttttctc
     6001 tagatgctaa taaattgaaa ctaatgaata taataacaca ggttttgata aattgtaaac
     6061 tcttactcaa aaaaagaatg ttaaaactaa tttaaccttt ttataaaggc tcatggcgtt
     6121 aaagaggata gtaaaaattc tacaattttt taatttaatg tcacaattgt cttgcgaata
     6181 taattctatt aattttcgct aactgctgtt ttagtactat tactttcaat atgccttata
     6241 tgcaacttta attcatgaag aatgcaatat ttttgcaatc tttcttgtac tgagcgcttt
     6301 ttatcattta gtactcatta tttaattttt cagtaaaggg caataaataa atttgtagaa
     6361 gatgcaatgt aatctcctct atccttttgc tcatatgttt atgagtatac ctagtctaga
     6421 aaggcttgta tttaaaatat tattcaataa aaattcacaa tttttacgac atgtgctacc
     6481 attcacttaa cttcctgatt ataaaattgg ttcgtttata ctaattactt aagtaccttt
     6541 aactaaacaa aatgcctata tatatattaa tttacaatga gtgtcagata agtcactatg
     6601 tccgagtggt taaggagtta gactcgccat cgatggacat ggaggcccag aataccctcc
     6661 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
     6721 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
     6781 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
     6841 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
     6901 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
     6961 catccgaaca taaacaacca tgcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     7021 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     7081 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     7141 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     7201 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggagcgg ccgcttcttc
     7261 ggtacaggtt atgttttttg gcaacagcaa ctattcaaat caactgaaag ttttttagaa
     7321 ttcaacaact tccaatactc aagtgtccaa gctagcaaac tgtttaataa tcgtgaccca
     7381 acattccttt catgcgcacc ctcagcgtca cataaatcaa cactttacaa cacctattca
     7441 aagcttctat atgccttcgt catttaaatg tctaacattt caaaaaagtg tttgctaatg
     7501 gtaaattctt gagttttctt ggaagcacat atataacgag tgtgactgtt ctacgctcct
     7561 tgctgactag tcatcagtct acgcatgcca actaccacca acataagcaa aaggtacccg
     7621 gggtaccgag ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     7681 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7741 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7801 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7861 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7921 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7981 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8041 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8101 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8161 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8221 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8281 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8341 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8401 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8461 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8521 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8581 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8641 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8701 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8761 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8821 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8881 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8941 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9001 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9061 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9121 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9181 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9241 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9301 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9361 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9421 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9481 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9541 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9601 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9661 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9721 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9781 tccccgaaaa gtgccacctg ac
//
