LOCUS       sequence-183246-        5438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Self-packages in TT-16L2 stable line.  L1-only capsid propagation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Buck CB, Thompson CD, Pang YY, Lowy DR, Schiller JT
  TITLE     Maturation of papillomavirus capsids.
  JOURNAL   J Virol. 2005 Mar;79(5):2839-46.
  PUBMED    15709003
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2005-03"; volume: "79(5)"; pages: "2839-46"
COMMENT     Sequence Label: p16L1-GFP
FEATURES             Location/Qualifiers
     source          1..5438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..89)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        74..403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      254..389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      428..437
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(434..436,437..439,440..1153)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(434..436,437..439,440..1150)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(479..500)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(740..759)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1087..1108
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1178..1299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1189..1208)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1243..1262
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1500..1711
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1724..1992
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     polyA_signal    3590..4162
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      4333..4921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4822..4841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5007..5054
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        5046..5064
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcaggactga ggataagaat tgtaacaaaa aaccccgccc cggcggggtt ttttgttaat
       61 taacctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
      121 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
      181 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
      241 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
      301 ctgactaatt ttttttattt atacagaggc cgaggccgcc tcggcctctg agctattcca
      361 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg attgggatcc
      421 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      481 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      541 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      601 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      661 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      721 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      781 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      841 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      901 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      961 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1021 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1081 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1141 gctgtacaag taaagcggcc gcttcgagca gacatgataa gatacattga tgagtttgga
     1201 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     1261 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     1321 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     1381 aaatgtggta aaatcgataa ggatccgggc tggcgtaata gcgaagaggc ccgcaccgat
     1441 cgcccttccc aacagttgcg gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg
     1501 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     1561 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     1621 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     1681 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     1741 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     1801 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     1861 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     1921 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     1981 ccgttacaga tccaagctgt gaccggcccg ctctagagcc accatgagcc tgtggctgcc
     2041 cagcgaggcc accgtgtacc tgccccccgt gcccgtgagc aaggtggtga gcaccgacga
     2101 gtacgtggcc aggaccaaca tctactacca cgccggcacc agcaggctgc tggccgtggg
     2161 ccacccctac ttccccatca agaagcccaa caacaacaag atcctggtgc ccaaggtgag
     2221 cggcctgcag tacagggtgt tcaggatcca cctgcccgac cccaacaagt tcggcttccc
     2281 cgacaccagc ttctacaacc ccgacaccca gaggctggtg tgggcctgcg tgggcgtgga
     2341 ggtgggcagg ggccagcccc tgggcgtggg catcagcggc caccccctgc tgaacaagct
     2401 ggacgacacc gagaacgcca gcgcctacgc cgccaacgcc ggcgtggaca acagggagtg
     2461 catcagcatg gactacaagc agacccagct gtgcctgatc ggctgcaagc cccccatcgg
     2521 cgagcactgg ggcaagggca gcccctgcac caacgtggcc gtgaaccccg gcgactgccc
     2581 ccccctggag ctgatcaaca ccgtgatcca ggacggcgac atggtggaca ccggcttcgg
     2641 cgccatggac ttcaccaccc tgcaggccaa caagagcgag gtgcccctgg acatctgcac
     2701 cagcatctgc aagtaccccg actacatcaa gatggtgagc gagccctacg gcgacagcct
     2761 gttcttctac ctgaggaggg agcagatgtt cgtgaggcac ctgttcaaca gggccggcgc
     2821 cgtgggcgag aacgtgcccg acgacctgta catcaagggc agcggcagca ccgccaacct
     2881 ggccagcagc aactacttcc ccacccccag cggcagcatg gtgaccagcg acgcccagat
     2941 cttcaacaag ccctactggc tgcagagggc ccagggccac aacaacggca tctgctgggg
     3001 caaccagctg ttcgtgaccg tggtggacac caccaggagc accaacatga gcctgtgcgc
     3061 cgccatcagc accagcgaga ccacctacaa gaacaccaac ttcaaggagt acctgaggca
     3121 cggcgaggag tacgacctgc agttcatctt ccagctgtgc aagatcaccc tgaccgccga
     3181 cgtgatgacc tacatccaca gcatgaacag caccatcctg gaggactgga acttcggcct
     3241 gcagcccccc cccggcggca ccctggagga cacctacagg ttcgtgacca gccaggccat
     3301 cgcctgccag aagcacaccc cccccgcccc caaggaggac cccctgaaga agtacacctt
     3361 ctgggaggtg aacctgaagg agaagttcag cgccgacctg gaccagttcc ccctgggcag
     3421 gaagttcctg ctgcaggccg gcctgaaggc caagcccaag ttcaccctgg gcaagaggaa
     3481 ggccaccccc accaccagca gcaccagcac caccgccaag aggaagaaga ggaagctgtg
     3541 aaagctaccc acggccgaat agccgtgagc cggaatcctg cacgctagca ttatccctaa
     3601 tacctgccac cccactctta atcagtggtg gaagaacggt ctcagaactg tttgtttcaa
     3661 ttggccattt aagtttagta gtaaaagact ggttaatgat aacaatgcat cgtaaaacct
     3721 tcagaaggaa aggagaatgt tttgtggacc actttggttt tcttttttgc gtgtggcagt
     3781 tttaagttat tagtttttaa aatcagtact ttttaatgga aacaacttga ccaaaaattt
     3841 gtcacagaat tttgagaccc attaaaaaag ttaaatgaga aacctgtgtg ttcctttggt
     3901 caacaccgag acatttaggt gaaagacatc taattctggt tttacgaatc tggaaacttc
     3961 ttgaaaatgt aattcttgag ttaacacttc tgggtggaga atagggttgt tttcccccca
     4021 cataattgga aggggaagga atatcattta aagctatggg agggttgctt tgattacaac
     4081 actggagaga aatgcagcat gttgctgatt gcctgtcact aaaacaggcc aaaaactgag
     4141 tccttgtgtt gcatagaaag cttcatgttg ctaaaccaat gttaagtgaa tctttggaaa
     4201 caaaatgttt ccaaattact gggatgtgca tgttgaaacg tgggttaatt aactagccat
     4261 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4321 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4381 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4441 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4501 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4561 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4621 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4681 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4741 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4801 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4861 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4921 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4981 gttcttaatt aaatttttca aaagtagttg acaattaatc atcggcatag tatatcggca
     5041 tagtataata cgactcacta taggagggcc atcatggcca agttgaccag tgctgtccca
     5101 gtgctcacag ccagggatgt ggctggagct gttgagttct ggactgacag gttggggttc
     5161 tccagagatt ttgtggagga tgactttgca ggtgtggtca gagatgatgt caccctgttc
     5221 atctcagcag tccaggacca ggtggtgcct gacaacaccc tggcttgggt gtgggtgaga
     5281 ggactggatg agctgtatgc tgagtggagt gaggtggtct ccaccaactt cagggatgcc
     5341 agtggccctg ccatgacaga gattggagag cagccctggg ggagagagtt tgccctgaga
     5401 gacccagcag gcaactgtgt gcactttgtg gcagagga
//
