LOCUS       sequence-183256-        9066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  It contains ~4.8 Kb of the miR-183-96-182 cluster promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Segura MF, Jubierre L, Li S, Soriano A, Koetz L, Gaziel-Sovran A, 
            Masanas M, Kleffman K, Dankert JF, Walsh MJ, Hernando E
  TITLE     Kruppel-like factor 4 (KLF4) regulates the miR-183~96~182 cluster 
            under physiologic and pathologic conditions.
  JOURNAL   Oncotarget. 2017 Apr 18;8(16):26298-26311. doi: 
            10.18632/oncotarget.15459.
  PUBMED    28412746
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget"; journalName: "Oncotarget"; date: "2017-04-18-
            18"; volume: "8"; issue: "16"; pages: "26298-26311"
COMMENT     Sequence Label: pGL4-Cluster Promoter Delta 2
FEATURES             Location/Qualifiers
     source          1..9066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..34
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        4926..4957
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      4984..4993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4990..6642
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    6686..6807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6697..6716)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6751..6770
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7055..7072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7226..7814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7306..7325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8014..8805,8806..8874))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    8979..9027
                     /note="synthetic polyadenylation signal"
     misc_feature    join(9041..9066,1..66)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
       61 ttctctggcc taactggccg gtaccgagct cggatccact agtaacggcc gccagtgtgc
      121 tggaattcgc ccttgaggct ggactcgatc ctttaacctg gcaggccacc ttctcctctg
      181 acttgggtag gggacccctc tggggtggac gcttatgttt cacctgctcg ggggtcaaag
      241 tcgaccgggg tgtcgcctgg taaaaagcag caagggccgc acccgcacgc ttctctctcc
      301 tctcgccttt gctccgagcg acgattctct actcatcgag gattcatggg agccaagagc
      361 ccgggcaagg cccgccgccc cagtgcctcc gctctgcagc tcgccctctg attcaactcc
      421 tgcaggcttc agcatcccga atgcttattt tattttattt tatttcattt tatttattga
      481 gacggagttt cgttcttgtt gcccaggctg gagtgcaatg gcgcgatctc ggctcaccgc
      541 aacctcctct ttccgggttc aagcgattct cctgcctcag cctccggagt agctggcatt
      601 acaggcgagt gccgccacac ccggctaatt ttgtattttt agtagagatg ggctttctcc
      661 atgttagtca cgctggttgg tctcgaactt ctgatctcag gtgatccacc cgcctcggcc
      721 tcccaaagag ctgggattgc aggcgtgagc cactgcgccc ggcccaggtt gctttttaaa
      781 aaatcaattt attggccggg cgcggtggct cacgcctgtg atcccaacac tttgggagac
      841 cgaggtgggc ggatcacgag gtcaggagat cgagaccatc ctgactaaca cggtgaaacc
      901 ccgcctctac taaaaaaaaa aaaaaaaaga aaaaaggaaa aaaaattagc cgggcgtggt
      961 ggcacgcgcc tgtaatccca gctactcggg agactgaggc aggagaatcg cttgagccca
     1021 ggaggcggag gttgcagtga gcctaaatag cgccactgca ctccagtctg ggctacagag
     1081 cgagactccg tctcaaaaca aacaaacaaa caaaaaacaa ttcctgcagt ttcttttttt
     1141 aattgaaaat tcaatgcaag aatatggtaa gcaacacaca ttggcttcga acagtcccga
     1201 aagacataca atgaaaagaa aaatctccaa ccctctgcgc tgagaccttt cgtgcagaaa
     1261 aaacctgatg tttaaccccc tgccccattt atatttttac acaaatagga atccacgtgc
     1321 cttttgcgtt tttcactgaa cgcctaggcg actttccagg atatataaag accccatgtt
     1381 ttcctcattc ttttaaagag ctgggtcgat tccgaagaca gaatctcctt tcatttgaca
     1441 gtctctgtgt ttctccttct ttcgaatctc tgcatcctcc gaccccgcag ccgggaagga
     1501 accactccag cctcccgtcc aaacagaacc ctacagggcc ctacctcgat cagaggaaaa
     1561 acgcacagaa gcacacgcat acggttttcg aaaaatgtcg ggaagttcgt tgggtttcca
     1621 gtgactttgt tgacccacga gaatctagat gctagagcaa aggaccgagg gctaagggct
     1681 gggccaagat cgaaagactc ccgctttggc ttccccggca cgtggccctg gcggaggaag
     1741 caccagggac acagcggctg gggcgggcac ccggcggcag tcgcgcggac gagggtgcag
     1801 ggcgtgctga ggccatctcg ctgggcctgc agtttgcaga gatctccggg tcgccaagta
     1861 cccacgtgct cacatgtgca ttttcttcat ccccagaggt gcgagtcatt ctgcccattt
     1921 tgcaaatggg aaactgaggc ttagagaggt gaagtgaatc cctcgagatc gagatcacag
     1981 agggactgga gcaggggagg actggaactc tgggttcctc tcactggggt tatactagtt
     2041 atgccatggg gccagggtta gcaggagaac ctcaccattc ggagaggggc caatggcaga
     2101 accacggccg catcagtggg tctgcgtgag gggcttgtat cccgggcacc tttcccccta
     2161 gtcctgccgc cggaggaatg ggaagggagg ggaagaccga tggttacttc tttgcccaag
     2221 gtcttcagca cgagtgggat tgtcatggag ctggcgaagg gagccgtgtg gggtagggac
     2281 taggagggag caagccagag ggacaagccg cgacaccatt aaaaaggacg gagcgcaagg
     2341 cgccgagtct cctaggtggg ggcagcaagg aagacagcgg gtctttccta aacctggcca
     2401 agcgtcgagg aacccaaaac tacccgtccc ggggcgcacg actccgggca gcgtgggacg
     2461 cctgtggcga ggcggaaggg atctctccag cgagagggaa tggggcaatt gccagacgga
     2521 cattggatct gcagaaggag ggggtaggat acacgcgggc ttaggatgcg ctggacaggc
     2581 cagcaaagtg aggaccccag gtcgtcctga gccacctctt cccccgcctg agccctgagc
     2641 cgtaagggat ccagggtatc aggtccccgg gggccgcccc ctcaccgtcc gagtccagct
     2701 ccggttacct gcctggcctg ctccttaaaa tatgtatgag gcccttgcgg gtgcagtttc
     2761 gcggcccctc ccggagtggg gctgggggac tcagggtgcg aggggagctg aggggggagg
     2821 aagcgacgcg agctccccgg ccggggcctt ctcgactttc agcagcgcat ttaagaaaag
     2881 gtggaaatcg gatctgtctc ggcggcgccc cagccccgcc cgctccgacg gccggacctc
     2941 gcggcccagc ggctagcttt ctgctgcgcg gtgtctggtt ctcgacagca ggtgaatggg
     3001 ccagggcggg gcgcccggag cgcggggggc ggggcgcgcg ttataaaggg gagcagcagg
     3061 ccgcgggggc cactcgcagt ccgcaaggcc tgcgcttccc gctttgcggt cctgggcaca
     3121 cccggcccgg gttgcccagc atcgcatggg tctgaggacc aggtagggcg cgggtgcaga
     3181 gacagccagg gaggctcgag gcgacctaga tgggcggggg aatgctagta ggacggggag
     3241 gggttaatgt ggagctatca cccccttttg gcccgttggg ctctgtgacg tgctcattat
     3301 ctccgctgtc cgggttctcg ctcaccgtgc ccatccctgg ctgcatgggg agaggggccc
     3361 cgaggccctg agactcgtcc agttatccac ctggggtggg aatcttgctt cccgatccat
     3421 ctggctgcga catctacggc ttcagcaggc cctgcccggg agtcgggcat attgaagggt
     3481 tcgtatcctt gccgtcaccc ttggcccctc ccggctcggc aggagctggg ggtggggaaa
     3541 ctatagattt cccgggcccc gcttccactg cctcaccccc ttcttttgct cgagcccaag
     3601 acaaggtgtt aacggtcttc aagtgcaagg gttaggtttg gtgggagcat aggctaagca
     3661 ccaccctcat tttacagggg cttctagggt gccagatctg cgggggtcga ttgggaagaa
     3721 ctagaagcta aaaaggagaa gggcggatta acgttttatt ttaatctggt tttgagctgc
     3781 cgttgccgtc tggggcggcc gggcggggcg gagctgggac tgccaagggc agccaaagca
     3841 gcagggaggg agggggcgct ccggtgctca ggatgctggg gaagaaggaa gaggccaccc
     3901 caacgctgac agcttggagg acctagcccc tgccccccgg tcccttctga atgctccggg
     3961 tggtgtcaga ccaaaggacg gttgagaagg atctaatccg cgtccgcgcc ccagccccgg
     4021 ctggccccgc cccctcccga gggagccact gtcgcagtgg ggggaggggg agatgggaac
     4081 gggtctgggg ggcggctccc ctccccctcc ctctcttggg acagacagcc tttgttcccg
     4141 gcggcggcgg cagcggtggc cagacacctg tgaggccgca gaactggccg agggtgggcg
     4201 tgtgaggaca tggaggcccc ttgtccccat tccagccctg cgcccctggg tccgggtgcg
     4261 ccccctgaga caggtcagcc ttgagcacct ttctgcgggg agactgcaga gatcgtccag
     4321 tggccctacg gtgcgcccca gctcccaacg cacttcccct cccccctccc agggtcggat
     4381 ttccaagcac gccacgccac acacacctcg gctagctccg ggctcagagg gcgctggggt
     4441 ggcagggcgt ccgggcgcac ctcggctagc ggcaccctag cccgagggca ggaggcgggg
     4501 atagaaggcg ggcgacggga ccccttgggg ccagacccgg ccgggttggg caagcaggcg
     4561 gggctcccgg cgtctccgca gtcccggacg ggcgtgccct gcgccgcgca ccaaccggag
     4621 ccacattcaa ggtgaatcat ccccggcccg ccccctgccc ctccccgcct cgcggcacct
     4681 gagccaatgg cgctgcgcga ggggcgggcg gggcccgggg cggggggcgg taaaaccctc
     4741 ctggagtcat tctctgtgca ctccgtcgag cccccgcgca gccgtgccgg agccgcaggg
     4801 cctccgtcca gccgcgcggt ccgggcagcc cgcgccccct gttcttccgg tcctcgggcc
     4861 caggtacctg agctcgctag cctcgaggat atcaagatct ggcctcggcg gccaagctta
     4921 gacactagag ggtatataat ggaagctcga cttccagctt ggcaatccgg tactgttggt
     4981 aaagccacca tggaagatgc caaaaacatt aagaagggcc cagcgccatt ctacccactc
     5041 gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc cctggtgccc
     5101 ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc cgagtacttc
     5161 gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac aaaccatcgg
     5221 atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc
     5281 atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct gctgaacagc
     5341 atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca aaagatcctc
     5401 aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag caagaccgac
     5461 taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc cggcttcaac
     5521 gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct gatcatgaac
     5581 agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc
     5641 cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga caccgctatc
     5701 ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg ctacttgatc
     5761 tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt gcgcagcttg
     5821 caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt cttcgctaag
     5881 agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag cggcggggcg
     5941 ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc aggcatccgc
     6001 cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga aggggacgac
     6061 aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac
     6121 accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc
     6181 atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa ggacggctgg
     6241 ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat cgtggaccgg
     6301 ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact ggagagcatc
     6361 ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc
     6421 ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac cgagaaggag
     6481 atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg tggtgttgtg
     6541 ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa gatccgcgag
     6601 attctcatta aggccaagaa gggcggcaag atcgccgtgt aataattcta gagtcggggc
     6661 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     6721 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6781 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6841 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     6901 atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     6961 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     7021 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7081 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7141 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7201 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7681 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7741 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7801 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7861 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7921 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7981 acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc
     8041 gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat
     8101 cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc
     8161 gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag
     8221 aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag
     8281 agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt
     8341 ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg
     8401 ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt
     8461 tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc
     8521 tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc
     8581 gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa
     8641 caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg
     8701 gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc
     8761 cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa
     8821 gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt
     8881 cctttttcaa tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt
     8941 aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat
     9001 tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa
     9061 acaaaa
//
