LOCUS       sequence-18332-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Bennett MJ, Huey-Tubman KE, Herr AB, West AP, Ross SA, Bjorkman PJ
  TITLE     Inaugural Article: A linear lattice model for polyglutamine in 
            CAG-expansion diseases.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9.
  PUBMED    12193654
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Sep 3. 99(18):11634-9."
COMMENT     Sequence Label: pET32a-HD25Q
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..325
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEI
                     ADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLD
                     ANLA"
     CDS             377..391
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             671..688
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gagcgataaa attattcacc tgactgacga cagttttgac acggatgtac tcaaagcgga
       61 cggggcgatc ctcgtcgatt tctgggcaga gtggtgcggt ccgtgcaaaa tgatcgcccc
      121 gattctggat gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat
      181 cgatcaaaac cctggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct
      241 gttcaaaaac ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa
      301 agagttcctc gacgctaacc tggccggttc tggttctggc gaacgccagc acatggacag
      361 cccagatctg ggtaccgacg acgacgacaa ggccatggcg accctggaaa agctgatgaa
      421 ggccttcgag tccctcaaaa gcttccaaca gcagcaacag caacaacagc agcaacagca
      481 acaacagcag caacagcaac aacagcagca acagcaacaa ccgccaccac ctccccctcc
      541 acccccacct cctcaacttc ctcaacctcc tccacaggca cagcctctgc tgcctcagcc
      601 acaacctcct ccacctccac ctccacctcc tccaggccca gctgtggctg aggagcctct
      661 gcaccgacct catcatcatc atcatcatta ggatccgaat tcgagctccg tcgacaagct
      721 tg
//
