LOCUS       sequence-183392-        1758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An ORF part-containing vector harbouring the signal peptide from 
            XynA with 4 bp overhangs specific for the 'signal peptide' position 
            within an ORF assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Gilbert C, Howarth M, Harwood CR, Ellis T
  TITLE     Extracellular Self-Assembly of Functional and Tunable Protein 
            Conjugates from Bacillus subtilis.
  JOURNAL   ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292.
  PUBMED    28230977
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292."
COMMENT     Sequence Label: pCG-A-xynA(SP)-B
FEATURES             Location/Qualifiers
     source          1..1758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(51..639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(131..150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             821..1480
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(887..906)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1551..1585
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
       61 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      121 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      181 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      241 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      301 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      361 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      421 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      481 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      541 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      601 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      661 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgattgat
      721 cgggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      781 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      841 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      901 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      961 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1021 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     1081 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     1141 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     1201 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtttcagc
     1261 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     1321 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     1381 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     1441 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg
     1501 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     1561 tcggttgccg ccgggcgttt tttattggtc tggtcggtct cggcttcctg cagaggcggt
     1621 tgccgaaaac aagctaatac tcattaaagc tgccgataat ccaactaaga aattcttttt
     1681 aaacttaaac atatcctgag accccgacta cggttatcca cagaatcagg ggataacgca
     1741 ggaaagaaca tgtgagca
//
