LOCUS       sequence-183608-       11413 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  I-SceI meganuclease-mediated mCherry transgenesis in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11413)
  AUTHORS   Ki DH, He S, Rodig S, Look AT
  TITLE     Overexpression of PDGFRA cooperates with loss of NF1 and p53 to 
            accelerate the molecular pathogenesis of malignant peripheral nerve 
            sheath tumors.
  JOURNAL   Oncogene. 2017 Feb 23;36(8):1058-1068. doi: 10.1038/onc.2016.269. 
            Epub 2016 Aug 1.
  PUBMED    27477693
REFERENCE   2  (bases 1 to 11413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2016.269"; journalName: "Oncogene"; date: "2017-02-23- 
            23"; volume: "36"; issue: "8"; pages: "1058-1068"
COMMENT     Sequence Label: sox10:mCherry
FEATURES             Location/Qualifiers
     source          1..11413
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2847..2871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2874..3581
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDALYK"
     primer_bind     complement(3016..3034)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3284..3303
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3583..3607)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3609..3743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3806..3826)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3834..3850)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3866..4000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4134..4152)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4185..4201)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4194..4216)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4343..4798
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4430..4449)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4640..4661
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4824..4928
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4929..4997,4998..5789)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5147..5166)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5960..6548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6449..6468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6702..6719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6836..6857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6872..6877,6878..6895,6896..6902)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6910..6926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6915..6937
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6934..6950
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6992..7012
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6994..7012
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     7049..7065
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7049..7065
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7099..7115)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7118..7134
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7154..7174
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aatgtaaggc aaaggaggat tatttaaata gaatgtcata cacaatggaa aatcaaagtg
       61 ttttacataa acaacaataa aagaaacata aataacaagc gtaaaagcaa attcaaactg
      121 aataataaaa aaataataaa gttgaagaca caaatgtata tttaaaaaag tttaagatct
      181 gaattaaaag taagatgccc ctgtgagagg ccaaatatta ctgtcgttga tttgcagccg
      241 aaatcaccgc cctctggggc aaaactcagc agccaatcag agcgggaccc gacagggctc
      301 tgttgctcct ttaagagatt tttttccatg aattgatttt tttctcgctc ttcacacaac
      361 ggggctcttt aagcctcgac gcgcgacaca gagcaggcat tcagagcgcg agcgaggggg
      421 ctgaaccgac ggactctcgt gctgggcggg cgacctgctg cactgtaaag taagtgcatt
      481 actggctttc tctctatcgc ttactctgtg tgggaatata tacgccattg cactgtctaa
      541 cggagaaact tttacatttc ttattagtaa taatactact aataataagc ggtgcattta
      601 taaacgacat ctacttattt tggatttttc ggtttatgta ttgatgggtt tggtcgtgga
      661 taattgtaat ggagaaggtt tttaattgaa atggattaaa ggacgtcttt aaaagtgcaa
      721 cttatctaat cttttggcga aaggaacact taaagttggt gtcacggggc ataaatttcg
      781 agatttgcgt gaatgtgtaa atcagagacg tgttgggtta atctgtctta attggaaaga
      841 acggcttaat ctatctgtgc acggaccact tgggatgtac atacactttt agatctttta
      901 gtaaatgttt acagtccgat aaattataca tttgaacact ttaattcgaa gtgaatcagt
      961 ttttaaatag ttttataaga aataattttt ttaaatttaa tcttctgaga agttttactt
     1021 gcgtaaggct atttgtatat atcgccgtct ctcgccaaat tgctcatgta accaacgcaa
     1081 agattaacaa agagaccaaa ctttaaagta cacaatttat aatattgttt atctacacat
     1141 cctttcacaa caaatataac aggatcagaa aacttaagta aagtaaaact aactgaaatg
     1201 cacatttgtg taagtttgaa tcaaatgttt gatacaataa taatctccgc agattagttt
     1261 gaaaggtttc attcgaatga gtttgtgtca tttatgagtt ttgtgttttg gcatgagtga
     1321 attaggggca aattattgcc aaaccaggcg ttatgatccg ccctacagca gccaaagatg
     1381 aacccaatca aatctgaaca aataaagttt acagcggagg aaactcaaat aagcaagagt
     1441 tgcgagcaaa gttaaaatga atgagcataa agtttataaa agtcctcctg acagaaaaca
     1501 gccgctgaaa tcttttcctt aaaacaaaat aaacccagca gcgcaattac cgaaattgac
     1561 ggatacattt gttagctact aaaacaaacc aagtcttgtg cgatgctttc tttttcgtcc
     1621 caaataaatt ggacaagtca aaccattaac gctttctgta ggctatacat gtgattaaca
     1681 aatgatcaat gtgttaatgt gttttgtaag gggtctacaa gtagccattc ttggctaact
     1741 ctagcacatt ttacagtaat gtttattaat gtgcattgac cctgtaaaca aataaactaa
     1801 acatggttct gctcatgtga agtcacctgt atcttaatta acactgctgt tggatgctgt
     1861 gtttttgtat tcgggttaat attggatgtc tgtaactcaa gttcaagctg acaggtcgca
     1921 tctcgacagc ttctgtcgca tataaattat ttgatcgatt tttctgtaat tggacctagt
     1981 caaccacatc cactcgttta attcacgatt acttcacatt tcagaaacac actgccatga
     2041 ctctcttgat ttttttcttc ttctgctggt tataagtcgt cgtttgtgct ttcaccaaca
     2101 aacactgtgt aattcagctg cccacggcag cgccgctccg tgtttatcca gcagcgaggc
     2161 ggaatgaagc cggtattatt ccagcacttt ctctcccggg agcttgaaca aaccgccaaa
     2221 ccttcaacag tagcttgtta gcagaagtga agctaaaata acacacacac atatacacac
     2281 accgaggccc actgacgcca ccgtatgatt gcagcgctgc cctggaaaaa ggcgacttct
     2341 aaataaagtc aaagccttag tgtttgtagg tttttcctga acaggtttat cttgttcatg
     2401 aggtgttcgg gtgaacagca aaggggttgt gcggtcatac agccaaccat taaaacgaca
     2461 tattaatgct attttataca ttaggaagac atgctctgca gaagcaagct gtttaataaa
     2521 ataataacaa tgattattat aactaataaa ttacctgtgt gtgtattttt gtaggcttcc
     2581 atcagatcta tatccagagg aagacggcgg aaggattcct tcttacaaac tcgaattata
     2641 taaaaagaac tgttaaggtt tcactggatg atcttaaata ataaacaaaa gcacaattat
     2701 tttacaagaa aaaaacattt gtaaagtata aattaataca tttatattta aaataaaatt
     2761 taagtgagga aattaaacct accgaagtca cctgtggccg cagaactagt tctagaacta
     2821 tagtgagtcg tattacgtag atccagacaa gtttgtacaa aaaagcaggc taagtgagca
     2881 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     2941 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     3001 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     3061 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     3121 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     3181 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     3241 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     3301 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     3361 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     3421 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     3481 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     3541 gccgccactc caccggcggc atggacgcgc tgtacaagta atacccagct ttcttgtaca
     3601 aagtggggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3661 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3721 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3781 agggggaggt gtgggaggtt ttttccaact ttattataca tagttgataa ttcactggcc
     3841 gtcgttttac ggtaccatcg atgatgatcc agacatgata agatacattg atgagtttgg
     3901 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     3961 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4021 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     4081 caaatgtggt atggctgatt atgatcctct agatcagatc gtacccaatt cgccctatag
     4141 tgagtcgtat tacgcgcgca ttaccctgtt atccctacgc gctcactggc cgtcgtttta
     4201 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4261 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4321 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4381 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4441 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4501 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4561 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4621 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4681 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4741 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     4801 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4861 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4921 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4981 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5041 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5101 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5161 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5221 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5281 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5341 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5401 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5461 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5521 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5581 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5641 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5701 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5761 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5821 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5881 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5941 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6001 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6061 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6121 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6181 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6241 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6301 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6361 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6421 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6481 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6541 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6601 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6661 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6721 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6781 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6841 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6901 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     6961 ccaagcgcgt agggataaca gggtaatgcg cgcaattaac cctcactaaa gggaacaaaa
     7021 gctggagctc caccgcggtg gcggccgctc tagaactagt ggatcccccg ggctgcagga
     7081 attcgatatc aagcttatcg ataccgtcga cctcgagcag gaaacagcta tgaccatgat
     7141 tacgccaagc tatcaacttt gtatagaaaa gttgtacgcc agtcgacggt atcgataagc
     7201 ttgatatcga attccccaca gcggacaaca ggacaaaacc tctcactgct acatgatcaa
     7261 tcctccaccc actgcccgct gcacttcctg tctcacacac acacacacac acaagcagag
     7321 ccagtgccat caggtgtgtg tgtgtgtgtg tcatgggaca tgcgtgtcct cctattgacc
     7381 ttctgacctc acaaacatct ttctctgaca tcccagagac catctccaac ggcaaccaac
     7441 cacatcatca ctgtcacatg ctgcatgact gcacacacac acacacacac tttctcgctc
     7501 acacaaacat attgattcac atttatacac acatttaaac acacaaacac atttaaatac
     7561 acacacacac acacacacac acatgatcct ataaactgcg cacaacacta agcaccagca
     7621 gttttagttt gctgaagcat cagtttgtaa cagaataact cagcctcctc atgatctcct
     7681 cttcgctgct cattcaggca aaactgcagt ttaaaagcgc tgataaacaa aactccaact
     7741 atggcacaca cactcccttg atcagagatg aattgatgaa attaattatg tttttgtgtg
     7801 cgtatttctt tattaaatca cgcagacgcg cgcagtttta tctctgcggt aatgcggatc
     7861 ataataaact cgtgattaaa aaactaatta cactgtcgca cgaacatgcg cgcattaaca
     7921 acgaacacac gagtcatttc gataaatata cagctcagaa attacattaa acatttataa
     7981 gatatcaaaa acggtcaaga gaaacgaatt tatttatgtt tttataaagc gcatttgcga
     8041 gtcgctcaaa aaactgtata gtatgaacat aataatgttt aaaaaacacg aaatactgat
     8101 acaaataata ctgttaataa taaaaacatt gttatttgta ttaaatagct tgtggatatc
     8161 cagaaataat gcgtaattct aaccgacaga tgatctaaaa tcatttcgtc agtctcttat
     8221 ttgatagact acaaatgcat aaaaagtaaa acgcgttttc gtatttatta aattggtttt
     8281 agtttttttt aattaaatta taatattata ataaaataat caattgtatt tttattactg
     8341 acatgcacgc ataataaacg cgctatacaa atgcgcatac acattacgca cctgcgcgtt
     8401 tgggttattt ccatttaaat aaattcaact acaaaacccc aaaacaaaaa gtttaaaaac
     8461 attttaattt ctgaatttaa aaaataaaca aaatcaatca tttaaaaatt gtaggcaggg
     8521 ttgtattaac ccaacgctgg gtcaaatatg gactcaaagt ttttgcatgg catttaaata
     8581 tttaatccgt ctgctgaatt tgtccatatt tgacaataat acatcccagc tattttagag
     8641 cgcatttctg atctcttctt taccataaaa acgaaacccc ttgataaagc agaatggcat
     8701 taaactggaa attaataata atgtaaacaa ttaactaata acatatataa aacaaaacga
     8761 tttacacaaa tagcgcagga cacatttccg cggtataaaa gaagcctccc tgttctgaca
     8821 ggcgatgcga cacacataac aaatcactct tctcaagcag ccacattaaa taacagaaga
     8881 aacaattaac atgcacgcgc atactaaaca gcacacatta acaaatgtcc ctcactgaca
     8941 aacacccccc agcttgatgt ttatgtccta taaatgctcg gagaccgagt gtcactttct
     9001 ccaaagcggg gtctcctcct tcccaaagcc tccctaagag caacagggca aaactcacat
     9061 caaagtgtgc agcaggcctc agatccgcgc gctttcccgt gtacatccac agacccgcga
     9121 gaaccgagac atgtgtgcgt gtacgtttga agggacaccg agaaaaagac gctctaaaga
     9181 gagacacacg tcttttctct tttcttttct ttttttatat acacagaact gctttttgtt
     9241 cctcattttt tcatttaaga gggtaatgga aggggggtgt tcgtttgatc acatgattac
     9301 aattcacctg agataaaaca aaggacgcaa agaaaggagc cgagcgccgg agaccgagac
     9361 agagagatgc tctcatggtc aggatcccct tatcagagtc aacattcatg gataatgaaa
     9421 aaggcaatga tttttggctt gggtgggggg gccgtcgtga gatggaaata caagaggtta
     9481 ttgttgacca gaacagcgcg cgtgtttgtg tgcgtttggc ccgtcgtttg cctctcttct
     9541 cgtctttcta tattttctac taaagctgtt taaacgcgtt aaatgcgctc tctgtgtgtg
     9601 tttatgcgcg ctgatgcaat tataacacta acacacatag cgcgacacga tgcaggagtt
     9661 cattccagtg ctgcgggcct gcgcgcgcag catgaacaca ccactgcgag caaatctgca
     9721 atattaatga atatatataa ctaaagtgtg cactttctgg tttataactg cagcaccccg
     9781 catacaccac agtataaagt aatctacaat attaatgaat ataataaaca agaatgttca
     9841 cttttaggtt taagaatgca ggcctgcgcg cgcagcatcc cgcgagcaca acactagaaa
     9901 ctaaactcca atattagtta atatattcaa ttaaaatgta cactttctgg tttaaaactg
     9961 caagtatgcg tgcgcagcat ccgacatacc caacactaga aactaatcta caatatttat
    10021 gaaagtattc aactaaaatg ttcactttct ggtttaaaac tgcaagtata cgcgtgcgca
    10081 gcatccgaca tacccaacac tagaaattaa tctaaattat taattaaagt attcaactga
    10141 attgttcact ttctggttta aaactgcaag tatgggtgcg cagcataaaa catacccaac
    10201 actagaaact aatctacaat attaatggat gtattcaact aaaatgttca ctttctggtt
    10261 taaaactgca ggaatgcgcg cgcaacaccc tgcggataac tttaaaaaca attccgcaat
    10321 cagctaaaac tgaaaagttc agttgattaa cgaatgtagg catgcgcaaa atattataaa
    10381 tgcgcacaca catctattta atacattaaa actaaggcta aatgttcact ttcaccattt
    10441 tggggaaaat gaaaattcag tgtccccctt aagtggccag ctttagggca ataggcgacc
    10501 ctccataagt agcataaaat cgtaggctat tctgggcacc ctttggcatc gccacgcgtt
    10561 aaacagggaa tggagaggtt attgcgcaca gcaggtgcgc agagtcgcgt ccgctaatgg
    10621 cgctggagct gctgacaggt ccgctggcgc tctgcggggt ggaagcgctc gaaaccccgc
    10681 acaacccctg attcaccctg aggcacagcc aggccaacaa cgggccagtg tgatcggaaa
    10741 agagttgaaa gaatctccgt gacaccaatg aggagccaaa gaccacctgt gaaatacatc
    10801 acatgtcaga caaaagcata ttaagctgat caaatattgc gtgcataacc gcattacatc
    10861 acaaagaaaa acattagcta gctatgggta acttaataag tgttctacat ttgcactgca
    10921 gccatggttg tgagccgaaa aggtttgcgc aataatattc accccccgag atttacattt
    10981 tttttttgcg gttctctgat gtcgattaat ggccacgtgg ccttcaggta aacacattta
    11041 atatttaact tatgtgtttt aattagtgtg ttccgaatta ttcggttatt tttatttaac
    11101 atttttatta attagcttaa ttctgatcca ctcccaactc ttattaacga atcacaaatc
    11161 aaatggatag ttttaattgc acaattagcc tgtcaaaact taacttaaag ctgttaatgc
    11221 actgctttta agtttgaaaa ataaagaaaa attattagaa aacttaagat tagattttaa
    11281 catatgttga aattatcaaa tcggcatgtt tatgataaaa caactttacc taaaactaaa
    11341 attcagatca acaaaaacct aaatgaagtt acatatatat atatatgtgt gtgtgtgtgt
    11401 gtatatatat ata
//
