LOCUS       sequence-183647-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_101 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_101
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    394..528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(418..437)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     472..491
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(639..655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(639..655)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(652..674)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    663..679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(687..693,694..711,712..717))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    732..753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggttccggt ggcggaggtt cccgaattcg tcgacggaga tctgatatga aaaagactcc
       61 atgggagaaa tgggaagtcg atttcttgcg cgaagtagcg gcgacaatgc cagttgaagt
      121 tatcgctgaa aaactggaaa ggactgaaaa agcagtaatg gcgaaagcaa caaggattgg
      181 cgctgacatt gttagccgac ttcgtggaag aagatggaca agagccgaag tatcactttt
      241 cggtaagttc tccgcagaag aaatagcaat tgcaacctgc cgctcaattt attcagtaag
      301 agctatgcga tacaagctaa aaaaactcga tgaagaaaga gcaggcatac gaataaattg
      361 aatcggtacc ctcgagaagc ttgcggccgc ggggatccag acatgataag atacattgat
      421 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      481 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      541 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggttttttc ggatcctcta
      601 gagtcgatct gcaggcatgc tagcttggcg taatcatggt catagctgtt tcctgtgtga
      661 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      721 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      781 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      841 ggtttgcgta ttggcgctct
//
