LOCUS       sequence-183650-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_106 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_106
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    324..458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(348..367)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     402..421
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(569..585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(569..585)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtcattacat atctggctga cgataacgcc agaaatcgac gcagagcacg cagacaggct
       61 caacgtgaac aggcaatgca agaacagcga ctggcgcgaa agattgcgct aaagctctct
      121 ggttgcgtca gagcagataa agcagcatca ctcgtaagcc ttcgctgcaa gaaggcagaa
      181 gaagtcgagc gtaaacagaa ccgtatttac taccgcaagc cacgcagtga aatgggtgtg
      241 acttgtgttg gtcgccagaa aatgaaatta ggcagcaaac cacttatttg aatcggtacc
      301 ctcgagaagc ttgcggccgc ggggatccag acatgataag atacattgat gagtttggac
      361 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      421 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      481 ttatgtttca ggttcagggg gaggtgtggg aggttttttc ggatcctcta gagtcgatct
      541 gcaggcatgc tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgtttat
      601 ccgctcacaa ttcca
//
