LOCUS       sequence-183656-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_464 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_464
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    861..994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(885..904)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     939..958
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg agcaaagcag ttattgtcat tccggctcgc
      121 tatggctcct cgcgcctgcc gggtaagcca ctgctcgata ttgttggtaa accgatgatc
      181 cagcatgttt acgagcgtgc gttacaggtg gcgggcgttg cggaagtttg ggtggcaaca
      241 gacgatcagc gtgttgaaaa ggccgtgcag gcatttggcg ggaaagccat catgacgcgc
      301 aacgatcatg aatccggcac cgatcggctg gtcgaggtga tgcataaagt cgaggcagat
      361 atctacatta acctgcaggg cgacgaacca atgattcgcc cgcgggatgt agaaacgctg
      421 ctacaaggaa tgcgtgatga tcccgcgttg ccagtggcaa cgctatgcca cgcgatttct
      481 gccgaagaag cgaccgagcc aagcacggta aaagtggtgg taaatacccg ccaggatgcg
      541 ctttatttca gccgttcgcc gattccctat ccgcgtaatg ctgaaaaagc gcgctacctg
      601 aaacacgttg gtatttacgc ttatcgtcgc gatgtgctgc aaaactacag tcagttaccg
      661 gaatccatgc cagagcaggc agaatcactg gagcagctgc ggttgatgag cgcagggatc
      721 aacatccgca catttgangt tgccgcaacc ggtccgggcg tcgacacccc agcatgcctg
      781 gaaaaagtgc gcgccctgat ggcacaggaa ctggctgaaa acgcatgaat cggtaccctc
      841 gagaagcttg cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa
      901 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      961 tatttgtaac cattatnagc tgcanaaaca agttaacana acanttgcat tcnttt
//
