LOCUS       sequence-183715-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_808 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_808
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    292..426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(316..335)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     370..389
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(537..553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(537..553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(550..572)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    561..577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    630..651
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(767..784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tggagggggc ggttccggtg gcggaggttc ccgaattctt cgacggaaat ctgatatgac
       61 atcaaaatac acttatcttc cggttgcgga tgaacgagct tcctggcgta gtcagtccat
      121 tatggcgctg gaaataacag cggatatcaa ctgcnagcgt gcnnctgaac gtgaccggca
      181 aaatttcctt tctgccagga agcgtttgca ggaacgtgtt aacnncgtgc tggcttccgg
      241 ggaggtatgt catggatgaa tcggtaccct cnagaagctt gcggccgcgg ggatccagac
      301 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      361 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      421 caagttaaca acaacaattg cattcatttt atgtttcagg ttcatgggga ggtgtgggag
      481 gttttttcgg atcctctana gtcgatctgc aggcatgcta ccttggcgta atcatggtca
      541 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat accagccgga
      601 agcataaagt gtanagcctg gggtgnctaa tgagtgagct aactcacatt aattgcgatg
      661 cgctcactgc ccgctttcca gtcnggaaac ctgtcgtgcc aactgcataa tgaatcggcc
      721 nacgcgcgcg gagaggcggt ttgcgnattg ggcgctcttc cgcttcctcg ctcactgact
      781 cgctgcgctc ggccgttcgg ctgcggngag cggtatcagc tcactcaaa
//
