LOCUS       sequence-183721-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_139 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_139
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    540..674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(564..583)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     618..637
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(786..802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(786..802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(799..821)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    810..826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(834..840,841..858,859..864))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    879..900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg aagaggatta tcggcgtcgt tgctggcgct
      121 atattgttat ctgggtgcgc aactattgtt ggtgacgaaa cacagcttgt gcaagtgaac
      181 agcaatcctt ccggtgcgag ctttaaggta aaagacgaat ccggcgtgat tgttgcgcaa
      241 ggtaagactc cgcaaggagt aactcttgcc aagtcagatg gtagttattt tggcaaaaag
      301 agctaccaga tcactatgga aaaggatggg tacgaaccag ttaccctgcc aatcaaagcc
      361 aatgctaatg gttggtatat tggtggaaac cttgtgtttg gtgggttaat tggttggctt
      421 gctgtagatc cttttaatgg tgggatgtat accttgaagc caaaagaggc aaacgcatct
      481 cttataccat caacaaagca agactgaatc ggtaccctcg agaagcttgc ggccgcgggg
      541 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      601 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      661 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
      721 tgtggggagg ttttttcgga tcctctagag tcgatctgca ggcatgctag cttggcgtaa
      781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      961 tgaatcggcc aacgcgcggg ga
//
