LOCUS       sequence-183729-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_044 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_044
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    318..452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(342..361)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     396..415
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(563..579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(563..579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(576..598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    587..603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(611..617,618..635,636..641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    656..677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(794..811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg ttgattttta ttttaaattg cgtggcggtt
      121 cagagcggat atatatatca ccataccatc atctgttttt ctgaagttca gattagtttt
      181 aacacactgt tttccatctt cgttgattac aacagtcttt ttaatgtatt tacgaatggc
      241 atcattcgac gttctaacgg atttagtcga tggtggggta attgaatcgg taccctcgag
      301 aagcttgcgg ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca
      361 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      421 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
      481 ttcaggttca gggggaggtg tgggaggttt tttcggatcc tctagagtcg atctgcaggc
      541 atgctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      601 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      661 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      721 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      901 agaacatgtg agcaaaaggc cagcaaaang ccaggaaccg taaaaaggcc gcgttgctgg
      961 cgtttttcca taggctccgc ccccctgacg agcatcacna aaaatcgacg ctcaagtca
//
