LOCUS       sequence-183730-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_698 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_698
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    504..638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(528..547)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     582..601
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(749..765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(749..765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(762..784)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    773..789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(797..803,804..821,822..827))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    842..863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(980..997)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcngcatgg acgagctgta cnagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg attgacatgc agcccgttcc ctttggcctg
      121 gttcttctga aatatctgca cactccacgt cgcgtggctc cgtttttagc gtgggctaag
      181 cgtctgggtg atgattatga agaaaccagc cggtgggaag ctcgggtgtt atcaaacgac
      241 ggattctatc ttgtccccaa ctcccgtctg acttatggcg taagcgtggc tgggaatggc
      301 tatgaaggca ccatgacggc agaggctttt ggcatcacgc tgtctttgtt tgccctcgga
      361 cagattgcga atacgactga agatgacagc gatatcctgc tctatcacgc gttacgtgat
      421 tacgcattgg atcactctga atcggtcgag attatggccg cgatagactg aatcggtacc
      481 ctcgagaagc ttgcggccgc ggggatccag acatgataag atacattgat gagtttggac
      541 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      601 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      661 ttatgtttca ggttcagggg gaggtgtggg aggttttttc ggatcctcta gagtcgatct
      721 gcaggcatgc tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      781 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc gggganaggc ggtttgcgta
      961 ttgggcgctc ttccgcntcc tcgctcactg actcgctgnn ctcggncntn cggctgcggc
     1021 gancggnatc agct
//
