LOCUS       sequence-183830-       12495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of human ID2 with 3xFLAG 
            N-terminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 12495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pSLIK TT 3xFLAG ID2 neo
FEATURES             Location/Qualifiers
     source          1..12495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        171..550
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        552..750
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             768..948
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    995..1120
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1613..1846
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2031..2075
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2373..2490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2603..2917
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2611..2629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2647..2665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2682..2700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2718..2736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2754..2772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2789..2807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2825..2843
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2890..2914
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2926..2991
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3438..3462)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3512..4723
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4560..4579
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4782..5399,5400..5405,5406..5441,5442..5444,5445..5480,
                     5481..5483,5484..5519,5520..5528)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5566..6138
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5732..5749)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5959..5978
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6154..6948
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6154..6948
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6208..6227)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6818..6837
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7116..7704
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7169..7189)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7587..7598)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7707..7723)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7708..7724)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8233..8413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8439..8456)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8445..8669
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8715..9143
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8802..8821)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9012..9033
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9152..9172)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9157..9486
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9337..9472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9399..9418
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9534..9581
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9600..9974
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10104..10225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10141..10160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10195..10214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10274..10290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10274..10290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10287..10309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10298..10314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10322..10328,10329..10346,10347..10352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10367..10388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10505..10522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10676..11264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10756..10775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11435..12226,12227..12295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12058..12077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12296..12400)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12475..12494)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
       61 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      121 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      181 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      241 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      301 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      361 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      421 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      481 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      541 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      601 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      661 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      721 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      781 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      841 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      901 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      961 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     1021 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1081 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1141 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1201 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1261 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1321 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1381 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1441 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1501 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1561 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1621 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1681 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1741 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1801 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1861 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1921 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1981 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2041 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2101 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2161 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2221 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2281 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     2341 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2401 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2461 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2521 gtttggttaa ttctagacag ttatcaacaa gtttgtacaa aaaagcaggc tttaaaggaa
     2581 ccaattcagt cgactggatc ccgagtttac tccctatcag tgatagagaa cgtatgtcga
     2641 gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca gtgatagaga
     2701 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     2761 cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg tatgtcgagt
     2821 ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt gggaggccta
     2881 tataagcaga gctcgtttag tgaaccgtca gatcgccact agatggacta caaagaccat
     2941 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gactagtatg
     3001 aaagccttca gtcccgtgag gtccgttagg aaaaacagcc tgtcggacca cagcctgggc
     3061 atctcccgga gcaaaacccc tgtggacgac ccgatgagcc tgctatacaa catgaacgac
     3121 tgctactcca agctcaagga gctggtgccc agcatccccc agaacaagaa ggtgagcaag
     3181 atggaaatcc tgcagcacgt catcgactac atcttggacc tgcagatcgc cctggactcg
     3241 catcccacta ttgtcagcct gcatcaccag agacccgggc agaaccaggc gtccaggacg
     3301 ccgctgacca ccctcaacac ggatatcagc atcctgtcct tgcaggcttc tgaattccct
     3361 tctgagttaa tgtcaaatga cagcaaagca ctgtgtggct gacaattgga agactaatgc
     3421 gttttcgaga tatctagacc cagctttctt gtacaaagtg gttgataaca tctcgagcca
     3481 taaagatggt taattaaccc acccaagatc tggcctccgc gccgggtttt ggcgcctccc
     3541 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
     3601 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
     3661 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
     3721 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
     3781 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
     3841 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
     3901 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc
     3961 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa
     4021 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt
     4081 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt
     4141 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt
     4201 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
     4261 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
     4321 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
     4381 tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca
     4441 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     4501 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     4561 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     4621 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
     4681 attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt ggtaccgagc
     4741 tcggatctcc accccgtacc ggtcctgcag tcgaattcac catgtctaga ctggacaaga
     4801 gcaaagtcat aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga
     4861 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
     4921 acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact
     4981 tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc
     5041 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
     5101 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
     5161 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
     5221 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
     5281 ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
     5341 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
     5401 ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
     5461 actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc
     5521 ccgggtaact aagtaaggat ccgcggccgc actagaggaa ttccgcccct ctcccccccc
     5581 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     5641 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     5701 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     5761 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     5821 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     5881 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     5941 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     6001 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     6061 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     6121 tgaaaaacac gatgataagc ttaccggtcc accatgattg aacaagatgg attgcacgca
     6181 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6241 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6301 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     6361 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6421 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6481 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6541 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6601 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6661 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     6721 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6781 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6841 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6901 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgatg tacaagtaaa
     6961 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7021 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7081 ttagtccctc ccaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     7141 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7201 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7261 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7321 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7381 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7441 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7501 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     7561 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7621 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7681 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa
     7741 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     7801 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     7861 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7921 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     7981 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     8041 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     8101 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     8161 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     8221 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8281 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8341 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8401 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8461 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8521 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8581 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8641 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8701 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8761 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8821 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8881 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8941 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     9001 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     9061 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     9121 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     9181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9241 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9301 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9361 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9421 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9481 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     9541 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     9601 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     9661 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     9721 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     9781 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     9841 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     9901 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     9961 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
    10021 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    10081 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    10141 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10201 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    10261 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    10321 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    10381 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10441 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10501 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10561 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10621 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10681 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10741 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10801 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10861 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10981 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    11041 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    11101 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11161 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11221 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11281 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11341 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11401 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11461 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11521 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11581 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11641 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11701 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11761 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11821 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11881 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    11941 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    12001 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    12061 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12121 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12181 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12241 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12301 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12361 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12421 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
    12481 ctgctctgat gccgc
//
