LOCUS       sequence-18483-e         291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVPT-rtTR-KRAB
FEATURES             Location/Qualifiers
     source          1..291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(106..125)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tggtggcgac cggtagcgct aggacgcgta agcttaggcc tggatcccgc tagaggtcga
       61 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
      121 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
      181 cccgcccccg gacccacccc ttcccaggcg ctgaacccag aaagcgaaag aacaaaactg
      241 ctattggccc ccgcccccaa ggcctacccg cttgcattgc gcacccgtgc t
//
