LOCUS       sequence-18533-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Hoover CL, Hilgenberg LG, Smith MA
  TITLE     The COOH-terminal domain of agrin signals via a synaptic receptor in
            central nervous system neurons.
  JOURNAL   J Cell Biol. 2003 Jun 9. 161(5):923-32.
  PUBMED    12796478
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2003 Jun 9. 161(5):923-32."
COMMENT     Sequence Label: C-Ag15
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             46..56
                     /estimated_length=11
     CDS             92..118
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             608..625
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctctttanc aatttatcag acaatcngtg tgggcactcg accggnnnnn nnnnnnaact
       61 ttattattaa aaattaaaga ggtatatatt aatgtatcga ttaaataagg aggaataaac
      121 catggatccg tggattggaa aggttggaga acgtgcagac tacatggctc tggccattgt
      181 ggatgggcac ctacaactga gctatgacct aggctcccag ccagttgtgc tgcgctccac
      241 tgtgaaggtc aacaccaacc gctggcttcg agtcagggct cacagggagc acagggaagg
      301 ttcccttcag gtgggcnatg aagcccctgt gactggctct tccccgctgg gtgccacaca
      361 attggacaca gatggagccc tgtggcttgg aggcctacag aagcttcctg tggggcaggc
      421 tctccccaag gcctatggca cgggttttgt gggctgtctg cgggacgtgg tagtgggcca
      481 tcgccagctg catctgctgg aggacgctgt cnccaaacca gagctaagac cctgccccac
      541 tctgaattcg aagcttgggc ccgaacaaaa actcatctca gaagangatc tgaatagcgc
      601 cgtcgaccat catcatcatc atcattgagt ttaaacggtc tccagcttgg cctgttttg
//
