LOCUS       sequence-18584-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  TITLE     Johnson Lab Plasmids
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-GFP-p48
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             310..318
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cccgctntcg gctgagagct gtcaggaatt cgcatcaggc agggcacgct gcacccgaaa
       61 actccggaac tgggtggtgg agcaagtgga gagtgggcag tttcccggag tgtgctggga
      121 tgatacagct aagaccatgt tccggattcc ctggaaacat gcaggcaagc aggacttccg
      181 ggaggaccag gatgctgcct tcttcaaggc ctgggcaata tttaagggaa agtataagga
      241 gggggacaca ggaggtccag ctgtctggaa gactcgcctg cgctgtgcac tcaacaagag
      301 ttctgaattt aaggaggttc ctgagagggg ccgcatggat gttgctgagc cctacaaggt
      361 gtatcagttg ctgccaccag gaatcgtctc tggccagcca gggactcaga aagtaccatc
      421 aaagcgacag cacagttctg tgtcctctga gaggaaggag gaagaggatg ccatgcagaa
      481 ctgcacactc agtccctctg tgctccagga ctccctcaat aatgaggagg agggggccag
      541 tgggggagca gtccattcag acattgggag cagcagcagc agcagcagcc ctgagccaca
      601 ggaagttaca gacacaactg aggccccctt tcaaggggat cagaggtccc tggagtttct
      661 gcttcctcca gagccagact actcactgct gctcaccttc atctacaacg ggcgcgtggt
      721 gggcgaggcc caggtgcaaa gcctggattg ccgccttgtg gctgagccct caggctctga
      781 gacagcatgg agcaggtgct gttcccaagc ctggccactg gagccacgca
//
