LOCUS       sequence-187743-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human TPK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: TPK1 gRNA (BRDN0001148855)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        97..172
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    228..346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
       61 aaaggacgaa acaccgcttc ggtgatatca tataaggttt tagagctaga aatagcaagt
      121 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttaa
      181 gcttggcgta actagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
      241 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      301 cnaactaaag aattacaaaa acaaattaca aancattcaa aattttcggg tttattacag
      361 ggacagcaga gatccacttt ggcgccngct cgagggggcc cgggtgcnaa gatggataaa
      421 gttttaaaca gagaggaatc tttgcagcta atggaccttc tangtcttga aaggagtggg
      481 aattggctcc ggngcccgtc agtgggcana gcgcacatcg cccacagtcc ccgagaagtt
      541 ggggggaggg gtcggcaatt gatccggtgc
//
