LOCUS       sequence-188215-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human CERKL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: CERKL gRNA (BRDN0001148191)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        148..223
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    279..396
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taataatttc ttgggtagtt tgcngtttta aaattatgtt ttaaaatgga ctatcatatg
       61 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      121 aacaccgggg gacgttaaca gcgccgggtt ttagagctag aaatagcaag ttaaaataag
      181 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttta agcttggcgt
      241 aactagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
      301 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
      361 gaattacaan aacaaattac aaanattcaa aattttcggg ntnattacag ggacagcaga
      421 natccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa gttttaaaca
      481 gacaggactc tttgnagcta atggaccttc taggtcttga aaggnntggg aantggctcc
      541 cgtgccccgt cngngggcag ancgcacatc
//
