LOCUS       sequence-188479-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human MAP3K1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: MAP3K1 gRNA (BRDN0001149426)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        82..157
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    213..330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
       61 cgcttctcac catatagccc tggtttagag ctagaaatag caagttaaaa taaggctagt
      121 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttaagcttg gcgtaactag
      181 atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
      241 tacagtgcan gggaaagaat agtagacata atagcaacag acatacaaac ttaagaatta
      301 caaaaacaaa ttacaaaaat tcnaaatttt cgggtttatt acagggacag cagagatcca
      361 ctttggcgcc ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg
      421 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
      481 cgtcagtggg cagagcgcac atcgcccaca gtccccgana acgttggggg gaggggtcgg
      541 caattgatcc ngtgcctaga gaangtggcg
//
