LOCUS       sequence-188499-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PMVK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PMVK gRNA (BRDN0001146309)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        146..221
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    277..394
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
       61 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      121 caccgcggaa ggacatgatc cgctggtttt agagctagaa atagcaagtt aaaataaggc
      181 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttaag cttggcgtaa
      241 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
      301 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      361 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
      421 tccactttgg cgccggctcg agggggcccg ggtgcaaaga tggataaagt tttaaacaga
      481 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      541 tgcccgtcag tgggcagagc gcacatcgcc
//
