LOCUS       sequence-189189-        3103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for cloning infectious RNA virus genomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Johnson KN, Zeddam JL, Ball LA
  TITLE     Characterization and construction of functional cDNA clones of 
            Pariacoto virus, the first Alphanodavirus isolated outside 
            Australasia.
  JOURNAL   J Virol. 2000 Jun;74(11):5123-32.
  PUBMED    10799587
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2000-06"; volume: "74(11)"; pages: "5123-32"
COMMENT     Sequence Label: TVT7R(0,0)
FEATURES             Location/Qualifiers
     source          1..3103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(406..424)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      420..467
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(535..553)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(536..553)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(843..862)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1072..1091)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1191..1213
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1251..1269)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1337..1441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1442..1510,1511..2302)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1660..1679)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2473..3061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2962..2981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
       61 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      121 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      181 attcattaat gcagggggat ctcgatcccg cgaaattaat acgactcact ataatgtctt
      241 cgaattcgat atcctcgagg aagacccggg tcggcatggc atctccacct cctcgcggtc
      301 cgacctgggc atccgaagga ggacgtcgtc cactcggatg gctaagggag agctcggatc
      361 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      421 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      481 ctatatccgg atcgagatcc tctagagtcg acctgcaggc atgcaagctt gtattctata
      541 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
      601 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
      661 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
      721 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
      781 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
      841 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
      901 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
      961 ctccttgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     1021 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     1081 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     1141 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     1201 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     1261 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1321 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1381 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1441 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1501 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1561 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1621 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1681 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1741 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1801 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1861 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1921 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1981 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     2041 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2101 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2161 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2221 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2281 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2341 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2401 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2461 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2521 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2581 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2641 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2701 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2761 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2821 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2881 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2941 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3001 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3061 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgc
//
