LOCUS       sequence-189192-        4436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of modified EBNA1 mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Howden SE, Wardan H, Voullaire L, McLenachan S, Williamson R, 
            Ioannou P, Vadolas J
  TITLE     Chromatin-binding regions of EBNA1 protein facilitate the enhanced 
            transfection of Epstein-Barr virus-based vectors.
  JOURNAL   Hum Gene Ther. 2006 Aug;17(8):833-44.
  PUBMED    16942443
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2006-08"; volume: "17(8)"; pages: "833-44"
COMMENT     Sequence Label: pSP6-EBNA(2A+DBD)
FEATURES             Location/Qualifiers
     source          1..4436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1506..1522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1506..1522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1519..1541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1530..1546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1554..1560,1561..1578,1579..1584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1599..1620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1737..1754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1908..2496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1988..2007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2667..3458,3459..3527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3290..3309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3528..3632)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3700..3718
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3756..3778)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3878..3897
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4111..4130
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(4420..4436,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(4420..4436,1)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaatacaagc ttggtcgacg agctcactag tcggcggccg ctctagagga tccccgggta
       61 cccggggatc tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa
      121 atggcctagg agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc
      181 aaagaagagg gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg
      241 gaagaccagg agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga
      301 gaccccaaaa acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag
      361 gaagatcttc cagtggacct caaagaagag ggggtgataa ccatggacga ggacggggaa
      421 gaggacgagg acgaggaggc ggaagaccag gagccccggg cggctcagga tcagggccaa
      481 gacatagaga tggtgtccgg agaccccaaa aacgtccaag ttgcattggc tgcaaaggga
      541 cccacggtgg aacaggagca ggaagatctt ctggatcttc atcatcatcc gggtctccac
      601 cgcgcaggcc ccctccaggt agaaggccat ttttccaccc tgtaggggaa gccgattatt
      661 ttgaatacca ccaagaaggt ggcccagatg gtgagcctga cgtgcccccg ggagcgatag
      721 agcagggccc cgcagatgac ccaggagaag gcccaagcac tggaccccgg ggtcagggtg
      781 atggaggcag gcgcaaaaaa ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca
      841 acccgaaatt tgagaacatt gcagaaggtt taagagctct cctggctagg agtcacgtag
      901 aaaggactac cgacgaagga acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga
      961 cctcccttta caacctaagg cgaggaactg cccttgctat tccacaatgt cgtcttacac
     1021 cattgagtcg tctccccttt ggaatggccc ctggacccgg cccacaacct ggcccgctaa
     1081 gggagtccat tgtctgttat ttcatggtct ttttacaaac tcatatattt gctgaggttt
     1141 tgaaggatgc gattaaggac cttgttatga caaagcccgc tcctacctgc aatatcaggg
     1201 tgactgtgtg cagctttgac gatggagtag atttgcctcc ctggtttcca cctatggtgg
     1261 aaggggctgc cgcggagggt gatgacggag atgacggaga tgaaggaggt gatggagatg
     1321 agggtgagga agggcaggag tgatgtaact tgttaggaga cgccctcaat cgtattaaaa
     1381 gccgtgtatt cccccgcact aaagaataaa tccccagtag acctgcaggc atgctctaga
     1441 ggatccccgg gcgagctccc aaaaaaaaaa aaaaaaaaaa aaaaaaaaac cgaattcgta
     1501 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1561 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1621 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1681 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1741 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1801 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1861 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1921 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1981 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2041 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2101 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2161 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2221 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2281 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2341 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2401 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2461 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2521 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2581 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2641 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2701 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2761 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2821 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2881 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2941 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3001 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3061 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3121 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3181 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3241 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3301 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3361 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3421 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3481 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3541 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3601 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3661 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3721 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3781 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3841 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     3901 agagtgcacc attcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     3961 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
     4021 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     4081 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     4141 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggctagc
     4201 gatgaccctg ctgattggtt cgctgaccat ttccgggtgc gggacggcgt taccagaaac
     4261 tcagaaggtt cgtccaacca aaccgactct gacggcagtt tacgagagag atgatagggt
     4321 ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg
     4381 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actata
//
