LOCUS       sequence-189288-       12272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-tagged Arabidopsis SEIPIN3 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 12272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pMDC43-SEIPIN3
FEATURES             Location/Qualifiers
     source          1..12272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        97..773
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             840..1865
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1906..2080
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2158..2182
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2607..3401
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3488..4076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3977..3996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4230..4247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4262..4402
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4328..4347)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4488..4510
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4746..4940
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5006..6079)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6508..7137)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8438..8462
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8650..8672
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8664..8681
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8665..8681
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9107..9451
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9519..9547)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10142..10162
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      11795..12047
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12064..12080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12064..12080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12077..12099)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12088..12104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12112..12118,12119..12136,12137..12142))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12157..12178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtattggcta gagcagcttg ccaacatggt ggagcacgac actctcgtct actccaagaa
       61 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      121 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      181 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      241 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      301 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaac atggtggagc
      361 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta
      421 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
      481 tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt
      541 gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac
      601 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
      661 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      721 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tgaaatcacc
      781 agtctctctc tacaaatcta tctctctcga gctttcgcag atccgggggg caatgagata
      841 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
      901 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
      961 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     1021 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     1081 gggagtttag cgagagcctg acctattgca tctcccgccg ttcacagggt gtcacgttgc
     1141 aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag gctatggatg
     1201 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     1261 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     1321 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     1381 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     1441 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     1501 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     1561 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccac
     1621 gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     1681 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     1741 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     1801 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaaga
     1861 aatagagtag atgccgaccg ggatctgtcg atcgacaagc tcgagtttct ccataataat
     1921 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
     1981 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     2041 ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta attcggcgtt
     2101 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     2161 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     2221 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     2281 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     2341 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     2401 agcgttgctg cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac
     2461 gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca
     2521 aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac
     2581 tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa
     2641 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
     2701 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag
     2761 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
     2821 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
     2881 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
     2941 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
     3001 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
     3061 ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt
     3121 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
     3181 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
     3241 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata
     3301 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     3361 agaaaataaa atattatatt ttactggatg aattgtttta gtacctagaa tgcatgacca
     3421 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3601 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3841 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3901 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4141 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4201 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4261 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     4321 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     4381 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     4441 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4501 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc
     4561 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta
     4621 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta
     4681 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag
     4741 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg
     4801 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa
     4861 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc
     4921 caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa
     4981 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt
     5041 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact
     5101 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc
     5161 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg
     5221 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat
     5281 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat
     5341 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc
     5401 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg
     5461 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt
     5521 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga
     5581 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat
     5641 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg
     5701 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt
     5761 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca
     5821 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa
     5881 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg
     5941 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg
     6001 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg
     6061 atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg
     6121 ccggcaggca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca
     6181 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa
     6241 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag
     6301 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat
     6361 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca
     6421 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt
     6481 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag
     6541 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc
     6601 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc
     6661 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga
     6721 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc
     6781 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa
     6841 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac
     6901 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc
     6961 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg
     7021 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt
     7081 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg
     7141 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc
     7201 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg
     7261 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc
     7321 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc
     7381 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg
     7441 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt
     7501 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa
     7561 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc
     7621 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc
     7681 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga
     7741 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg
     7801 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct
     7861 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg
     7921 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt
     7981 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt
     8041 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc
     8101 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact
     8161 ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc
     8221 gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga
     8281 agccgactgc actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac
     8341 cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa
     8401 atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt
     8461 caaacactga tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc
     8521 tctagcattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     8581 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     8641 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc
     8701 aggtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
     8761 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
     8821 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
     8881 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
     8941 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     9001 ccacgtcttc aaagcaagtg gattgatgtg ataacatggt ggagcacgac acacttgtct
     9061 actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac
     9121 aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg
     9181 tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     9241 ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     9301 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     9361 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
     9421 tataaggaag ttcatttcat ttggagagga cctcgactct agaggatccc cgggtaccgg
     9481 tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     9541 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     9601 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     9661 caaccctggt caccaccctg acctacggcg tgcagtgctt ctcccgttac cctgatcata
     9721 tgaagcggca cgacttcttc aagagcgcca tgcctgaggg atacgtgcag gagaggacca
     9781 tcttcttcaa ggacgacggg aactacaaga cacgtgctga agtcaagttt gagggagaca
     9841 ccctcgtcaa caggatcgag cttaagggaa tcgatttcaa ggaggacgga aacatcctcg
     9901 gccacaagtt ggaatacaac tacaactccc acaacgtata catcatggcc gacaagcaaa
     9961 agaacggcat caaagccaac ttcaagaccc gccacaacat cgaagacggc ggcgtgcaac
    10021 tcgctgatca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
    10081 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
    10141 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
    10201 aaggcgcgcc aatggaatca gaatcagaat catcatcaac acatagcagc tgcgatcgat
    10261 tcctagacgc agaagacgaa ttcttttacg atagcttctc gaaccactac gactgcctca
    10321 attcgtctcc ccccgcgaat ctccgtcgcc gaaggttgcc tatggatacg gattcttctt
    10381 cttcctcttc tacgtcttca ttggaatctt gtgagaaacg gagcaccgtg ggagaaaatg
    10441 acgagcttga ggtttctcta gtggatttcg aaacaattga aattgatgtc gatgttactg
    10501 actctgcaaa tagctcaatc gactcgattt cggaaaaagg agaagacttt gaagtaattg
    10561 actcgtgtac cgatacggag aagaatatgg gtgagaatga ttcgggtcgg gtcgacccgt
    10621 ttacagtgac caccttgaat gatgagcgag gtgagattta taccggaccg gaatatactt
    10681 cgacggattg gtctttgacg agtctagtga tcaggtcaat tgaatttcaa gttagtttga
    10741 tgattacctt tataaggttt cctccatggt taattagtaa atgtttgagc tttgtttttg
    10801 atccgtatcg aacaatgagg cgtggtagac gctatttagt atcgtggatt gttgggttat
    10861 gtgattcagg gttaaaagat gataagcctg tgttggagct tgtgcgtaga gtaacatggg
    10921 gtttgttttg tgctgtttat gtggggatca tgttgtttgc cttgttggtt tctgcgttta
    10981 tgattagtgg ttttgtgatt acctatttgg ctcatgaacc acttgttatc aaagagagct
    11041 tgaattttga ttacaccaag agtagtcctg aagcttatgt gcccataagt tcatgtgctg
    11101 gtgttgcctt tggtttgagt ggcaaggaga gtattgaaac tgggaaggtc aaagggttga
    11161 aagacaggac agagataact gtgtcaatga cattgcctga gtccgagtac aatagaaatc
    11221 tcgggatgtt tcaggttcgg gtggacttct tatccgcgag tggtcatgtg cttgctagtt
    11281 caaggcggcc gtgtatggta aaatttagta gcgagcctat acgtcttgtc cagactttgc
    11341 taaagattgc tccgcttgtc acgggctatg tctctgaaat ccaaaccttg aacttgaagc
    11401 ttaaaggctt ggtcgaaaag gacattattc ctactgcttg cttgaagatc atgatcgaac
    11461 aacgtgcaga gtttcgtcct ggagcaggta ttcctgagat ttacgatgca tctctgttcc
    11521 ttgaatccaa acttcctttc ttgaaaagga tcatatggaa ctggaggaaa actttgtttg
    11581 tctggatcag catgagtttg ttcattatgg aattactttt cgccttagtt ttcttcagac
    11641 ctctgatcat cccaagaaca ggtcagagaa cacaacaaag agatggaaca cattctatca
    11701 acaacaactt atatcttgac ggtcaggcgg gaagtagatg attaattaac tagttctaga
    11761 gcggccgcca ccgcggtgga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
    11821 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
    11881 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
    11941 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
    12001 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattcgtaat
    12061 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    12121 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    12181 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    12241 aatcggccaa cgcgcgggga gaggcggttt gc
//
