LOCUS       sequence-189304-       13047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-tagged Arabidopsis SEIPIN1 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13047)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 13047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pMDC84-SEIPIN1
FEATURES             Location/Qualifiers
     source          1..13047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        151..495
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1694..1722)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2317..2337
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2378..2395
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2415..2667
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2684..2700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2684..2700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2697..2719)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2708..2724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2732..2738,2739..2756,2757..2762))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2777..2798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2989..3665
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3732..4757
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4798..4972
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5050..5074
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5499..6293
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7579..8167
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8068..8087
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8321..8338
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8353..8493
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8419..8438)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8579..8601
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8837..9031
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9097..10170)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10599..11228)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12529..12553
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     12741..12763
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12755..12772
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12756..12772
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
       61 agtggattga tgtgataaca tggtggagca cgacacactt gtctactcca aaaatatcaa
      121 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg
      181 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
      241 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
      301 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
      361 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
      421 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
      481 tcatttggag aggacctcga ctctagaact agttaattaa atgagaatcc tccaaaacaa
      541 aacaatgaaa gaacaagaca accaactcaa aatccctgaa ccattacgag ctgactggtt
      601 catggttcta gtcaccatcc aagctgatct aatctacaac gcactcgtgg tattatcctc
      661 acctttcttt ctcctatacc gctcttaccg ccgcgcagtc gttaccgtct ccgcagcaga
      721 aaaagcggtt aaacgtgctc cagcgcagat agctggtggt gcggggcggg ttgttaggag
      781 gacatggttt ggtatattgg gtgcatgcca cgtgtcaatg gtaatggtgt tggctttgat
      841 tttggcggtt gttattggtg ttgggattgt gagtttgtat gtagaaaagc ctgtggttgt
      901 gagagacaga ttgttctttg attacactga agaaaacccc agtgcggttt ttagttttga
      961 taagaagaag agatcgttta gtgttcctgt tggtcatagt gttcatgtct ctttagtgct
     1021 ttggatgcct gaatctgaaa ttaatcgaag aattggagta tttcagttga aagtagagct
     1081 tttatcgttg aaaggcgaga caatcgcgag atcgagccaa ccttgtatgt tacggttcag
     1141 aagcaaacct ataagacttg cacgaacatt tgtgatgagt gttccactta tagccgggat
     1201 agcaaacgaa gcacaaacaa tgagaatcga tgcattaaag caccaagaga aaatgccaag
     1261 gacaaaagcc gtgagagcga ctctgattcc gcgtgcacaa actcggacat tgcctcagtt
     1321 atatgaagca gagatcgtta taaactcaaa accgccctgg attaaacgaa tggcttataa
     1381 ctggaaatgg actctttgcg tatggacttc aatgtacctt tacgtcgcga ttttgaccgc
     1441 acttctttgg tgttttagac cggttttgtt cccttacact tcctcaagaa ccatttcgga
     1501 gagcgaaaac ttagagatcg aagtagttga agaagaacaa gaacaagtca tggaaagacg
     1561 acggagggaa agaagaaacc agcctcgtcg aagaaatttt gctactacac agaaaagtta
     1621 tacatggcgc gcctcgaggg ggggcccggt accggtagaa aaaatgagta aaggagaaga
     1681 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     1741 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     1801 tatttgcact actggaaaac tacctgttcc atggccaacc ctggtcacca ccctgaccta
     1861 cggcgtgcag tgcttctccc gttaccctga tcatatgaag cggcacgact tcttcaagag
     1921 cgccatgcct gagggatacg tgcaggagag gaccatcttc ttcaaggacg acgggaacta
     1981 caagacacgt gctgaagtca agtttgaggg agacaccctc gtcaacagga tcgagcttaa
     2041 gggaatcgat ttcaaggagg acggaaacat cctcggccac aagttggaat acaactacaa
     2101 ctcccacaac gtatacatca tggccgacaa gcaaaagaac ggcatcaaag ccaacttcaa
     2161 gacccgccac aacatcgaag acggcggcgt gcaactcgct gatcattatc aacaaaatac
     2221 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     2281 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     2341 tgctgggatt acacatggca tggtagaact atacaaacac caccaccacc accactaaga
     2401 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     2461 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     2521 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     2581 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     2641 gcgcggtgtc atctatgtta ctagatcggg aattcgtaat catgtcatag ctgtttcctg
     2701 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2761 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2821 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2881 gaggcggttt gcgtattggc tagagcagct tgccaacatg gtggagcacg acactctcgt
     2941 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     3001 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     3061 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     3121 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     3181 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     3241 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     3301 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     3361 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
     3421 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
     3481 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     3541 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     3601 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac
     3661 gctgaaatca ccagtctctc tctacaaatc tatctctctc gagctttcgc agatccgggg
     3721 ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     3781 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     3841 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     3901 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     3961 tgcttgacat tggggagttt agcgagagcc tgacctattg catctcccgc cgttcacagg
     4021 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctacaa ccggtcgcgg
     4081 aggctatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     4141 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     4201 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     4261 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     4321 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     4381 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     4441 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     4501 caggatcgcc acgactccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     4561 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     4621 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     4681 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     4741 cgagggcaaa gaaatagagt agatgccgac cgggatctgt cgatcgacaa gctcgagttt
     4801 ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg
     4861 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
     4921 tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat
     4981 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     5041 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     5101 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     5161 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     5221 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
     5281 taacgatgac agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat
     5341 actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg
     5401 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
     5461 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
     5521 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
     5581 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
     5641 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
     5701 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
     5761 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
     5821 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
     5881 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
     5941 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
     6001 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
     6061 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
     6121 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
     6181 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     6241 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
     6301 ggaaggtgcg aataagcggg gaaattcttc tcggctgact cagtcatttc atttcttcat
     6361 gtttgagccg attttttctc ccgtaaatgc cttgaatcag cctatttaga ccgtttcttc
     6421 gccatttaag gcgttatccc cagtttttag tgagatctct cccactgacg tatcatttgg
     6481 tccgcccgaa acaggttggc cagcgtgaat aacatcgcca gttggttatc gtttttcagc
     6541 aaccccttgt atctggcttt cacgaagccg aactgtcgct tgatgatgcg aaatgggtgc
     6601 tccaccctgg cccggatgct ggctttcatg tattcgatgt tgatggccgt tttgttcttg
     6661 cgtggatgct gtttcaaggt tcttaccttg ccggggcgct cggcgatcag ccagtccaca
     6721 tccacctcgg ccagctcctc gcgctgtggc gccccttggt agccggcatc ggctgagaca
     6781 aattgctcct ctccatgcag cagattaccc agctgattga ggtcatgctc gttggccgcg
     6841 gtggtgacca ggctgtgggt caggccactc ttggcatcga caccaatgtg ggccttcatg
     6901 ccaaagtgcc actgattgcc tttcttggtc tgatgcatct ccggatcgcg ttgctgctct
     6961 ttgttcttgg tcgagctggg tgcctcaatg atggtggcat cgaccaaggt gccttgagtc
     7021 atcatgacgc ctgcttcggc cagccagcga ttgatggtct tgaacaattg gcgggccagt
     7081 tgatgctgct ccagcaggtg gcggaaattc atgatggtgg tgcggtccgg caaggcgcta
     7141 tccagggata accgggcaaa cagacgcatg gaggcgattt cgtacagagc atcttccatc
     7201 gcgccatcgc tcaggttgta ccaatgctgc atgcagtgaa tgcgtagcat ggtttccagc
     7261 ggataaggtc gccggccatt accagccttg gggtaaaacg gctcgatgac ttccaccatg
     7321 ttttgccatg gcagaatctg ctccatgcgg gacaagaaaa tctcttttct ggtctgacgg
     7381 cgcttactgc tgaattcact gtcggcgaag gtaagttgat gactcatgat gaaccctgtt
     7441 ctatggctcc agatgacaaa catgatctca tatcagggac ttgttcgcac cttccctaga
     7501 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7561 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7621 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7681 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     7741 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7801 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7861 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7921 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7981 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8041 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8101 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8161 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8221 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8281 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8341 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     8401 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     8461 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     8521 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8581 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     8641 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     8701 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     8761 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     8821 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     8881 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     8941 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     9001 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     9061 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     9121 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     9181 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     9241 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     9301 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     9361 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     9421 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     9481 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     9541 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     9601 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     9661 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     9721 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     9781 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     9841 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     9901 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     9961 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
    10021 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
    10081 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
    10141 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
    10201 ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca
    10261 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
    10321 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    10381 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    10441 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    10501 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    10561 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    10621 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    10681 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    10741 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    10801 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    10861 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    10921 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    10981 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    11041 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    11101 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    11161 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    11221 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    11281 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    11341 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    11401 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    11461 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    11521 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    11581 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    11641 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    11701 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    11761 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    11821 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    11881 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    11941 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    12001 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    12061 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    12121 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    12181 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    12241 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    12301 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    12361 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc
    12421 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca
    12481 cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca
    12541 atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa
    12601 gctcaagctg ctctagcatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    12661 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    12721 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    12781 tgcatgcctg caggtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa
    12841 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg
    12901 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
    12961 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
    13021 ctctgccgac agtggtccca aagatgg
//
