LOCUS       sequence-189309-       13446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mutated Arabidopsis SEIPIN1 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13446)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 13446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pMDC84-SEIPIN3N-1C
FEATURES             Location/Qualifiers
     source          1..13446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        444..788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(2386..2414)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3009..3029
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3070..3087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3107..3359
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3376..3392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3376..3392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3389..3411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3400..3416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3424..3430,3431..3448,3449..3454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3469..3490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3681..4357
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4424..5449
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5490..5664
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5742..5766
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6191..6985
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8271..8859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8760..8779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9013..9030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9045..9185
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9111..9130)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9271..9293
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9529..9723
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9789..10862)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11291..11920)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13221..13245
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(13433..13446,1..9)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtaaaacga cggccagtgc caagcttgca tgcctgcagg tcaacatggt ggagcacgac
       61 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
      121 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
      181 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
      241 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
      301 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
      361 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
      421 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
      481 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
      541 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
      601 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
      661 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
      721 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
      781 gagaggacct cgactctaga actagttaat taaatggaat cagaatcaga atcatcatca
      841 acacatagca gctgcgatcg attcctagac gcagaagacg aattctttta cgatagcttc
      901 tcgaaccact acgactgcct caattcgtct ccccccgcga atctccgtcg ccgaaggttg
      961 cctatggata cggattcttc ttcttcctct tctacgtctt cattggaatc ttgtgagaaa
     1021 cggagcaccg tgggagaaaa tgacgagctt gaggtttctc tagtggattt cgaaacaatt
     1081 gaaattgatg tcgatgttac tgactctgca aatagctcaa tcgactcgat ttcggaaaaa
     1141 ggagaagact ttgaagtaat tgactcgtgt accgatacgg agaagaatat gggtgagaat
     1201 gattcgggtc gggtcgaccc gtttacagtg accaccttga atgatgagcg aggtgagatt
     1261 tataccggac cggaatatac ttcgacggat tggtctttga cgagtctagt gatcaggtca
     1321 ctaatctaca acgcactcgt ggtattatcc tcacctttct ttctcctata ccgctcttac
     1381 cgccgcgcag tcgttaccgt ctccgcagca gaaaaagcgg ttaaacgtgc tccagcgcag
     1441 atagctggtg gtgcggggcg ggttgttagg aggacatggt ttggtatatt gggtgcatgc
     1501 cacgtgtcaa tggtaatggt gttggctttg attttggcgg ttgttattgg tgttgggatt
     1561 gtgagtttgt atgtagaaaa gcctgtggtt gtgagagaca gattgttctt tgattacact
     1621 gaagaaaacc ccagtgcggt ttttagtttt gataagaaga agagatcgtt tagtgttcct
     1681 gttggtcata gtgttcatgt ctctttagtg ctttggatgc ctgaatctga aattaatcga
     1741 agaattggag tatttcagtt gaaagtagag cttttatcgt tgaaaggcga gacaatcgcg
     1801 agatcgagcc aaccttgtat gttacggttc agaagcaaac ctataagact tgcacgaaca
     1861 tttgtgatga gtgttccact tatagccggg atagcaaacg aagcacaaac aatgagaatc
     1921 gatgcattaa agcaccaaga gaaaatgcca aggacaaaag ccgtgagagc gactctgatt
     1981 ccgcgtgcac aaactcggac attgcctcag ttatatgaag cagagatcgt tataaactca
     2041 aaaccgccct ggattaaacg aatggcttat aactggaaat ggactctttg cgtatggact
     2101 tcaatgtacc tttacgtcgc gattttgacc gcacttcttt ggtgttttag accggttttg
     2161 ttcccttaca cttcctcaag aaccatttcg gagagcgaaa acttagagat cgaagtagtt
     2221 gaagaggaac aagaacaagt catggaaaga cgacggaggg aaagaagaaa ccagcctcgt
     2281 cgaagaaatt ttgctactac acagaaaagt tatacatggc gcgcctcgag ggggggcccg
     2341 gtaccggtag aaaaaatgag taaaggagaa gaacttttca ctggagttgt cccaattctt
     2401 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     2461 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     2521 ccatggccaa ccctggtcac caccctgacc tacggcgtgc agtgcttctc ccgttaccct
     2581 gatcatatga agcggcacga cttcttcaag agcgccatgc ctgagggata cgtgcaggag
     2641 aggaccatct tcttcaagga cgacgggaac tacaagacac gtgctgaagt caagtttgag
     2701 ggagacaccc tcgtcaacag gatcgagctt aagggaatcg atttcaagga ggacggaaac
     2761 atcctcggcc acaagttgga atacaactac aactcccaca acgtatacat catggccgac
     2821 aagcaaaaga acggcatcaa agccaacttc aagacccgcc acaacatcga agacggcggc
     2881 gtgcaactcg ctgatcatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     2941 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     3001 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggtagaa
     3061 ctatacaaac accaccacca ccaccactaa gagctcgaat ttccccgatc gttcaaacat
     3121 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     3181 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     3241 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     3301 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     3361 ggaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3421 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3481 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3541 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg gctagagcag
     3601 cttgccaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc
     3661 tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
     3721 ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc
     3781 acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac
     3841 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
     3901 accacgtctt caaagcaagt ggattgatgt gaacatggtg gagcacgaca ctctcgtcta
     3961 ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca
     4021 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa
     4081 aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc
     4141 tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     4201 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
     4261 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
     4321 ataaggaagt tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa
     4381 tctatctctc tcgagctttc gcagatccgg ggggcaatga gatatgaaaa agcctgaact
     4441 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     4501 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     4561 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     4621 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt ttagcgagag
     4681 cctgacctat tgcatctccc gccgttcaca gggtgtcacg ttgcaagacc tgcctgaaac
     4741 cgaactgccc gctgttctac aaccggtcgc ggaggctatg gatgcgatcg ctgcggccga
     4801 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     4861 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     4921 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     4981 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     5041 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     5101 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     5161 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccacgactcc gggcgtatat
     5221 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     5281 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     5341 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     5401 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aagaaataga gtagatgccg
     5461 accgggatct gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca
     5521 gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     5581 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     5641 aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca gtacattaaa
     5701 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     5761 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     5821 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     5881 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     5941 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     6001 atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca
     6061 actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg
     6121 agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag
     6181 gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata
     6241 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga
     6301 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt
     6361 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct
     6421 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct
     6481 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga
     6541 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga
     6601 cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga
     6661 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac
     6721 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt
     6781 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa
     6841 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta
     6901 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta
     6961 tattttactg gatgaattgt tttagtacct agggaaggtg cgaataagcg gggaaattct
     7021 tctcggctga ctcagtcatt tcatttcttc atgtttgagc cgattttttc tcccgtaaat
     7081 gccttgaatc agcctattta gaccgtttct tcgccattta aggcgttatc cccagttttt
     7141 agtgagatct ctcccactga cgtatcattt ggtccgcccg aaacaggttg gccagcgtga
     7201 ataacatcgc cagttggtta tcgtttttca gcaacccctt gtatctggct ttcacgaagc
     7261 cgaactgtcg cttgatgatg cgaaatgggt gctccaccct ggcccggatg ctggctttca
     7321 tgtattcgat gttgatggcc gttttgttct tgcgtggatg ctgtttcaag gttcttacct
     7381 tgccggggcg ctcggcgatc agccagtcca catccacctc ggccagctcc tcgcgctgtg
     7441 gcgccccttg gtagccggca tcggctgaga caaattgctc ctctccatgc agcagattac
     7501 ccagctgatt gaggtcatgc tcgttggccg cggtggtgac caggctgtgg gtcaggccac
     7561 tcttggcatc gacaccaatg tgggccttca tgccaaagtg ccactgattg cctttcttgg
     7621 tctgatgcat ctccggatcg cgttgctgct ctttgttctt ggtcgagctg ggtgcctcaa
     7681 tgatggtggc atcgaccaag gtgccttgag tcatcatgac gcctgcttcg gccagccagc
     7741 gattgatggt cttgaacaat tggcgggcca gttgatgctg ctccagcagg tggcggaaat
     7801 tcatgatggt ggtgcggtcc ggcaaggcgc tatccaggga taaccgggca aacagacgca
     7861 tggaggcgat ttcgtacaga gcatcttcca tcgcgccatc gctcaggttg taccaatgct
     7921 gcatgcagtg aatgcgtagc atggtttcca gcggataagg tcgccggcca ttaccagcct
     7981 tggggtaaaa cggctcgatg acttccacca tgttttgcca tggcagaatc tgctccatgc
     8041 gggacaagaa aatctctttt ctggtctgac ggcgcttact gctgaattca ctgtcggcga
     8101 aggtaagttg atgactcatg atgaaccctg ttctatggct ccagatgaca aacatgatct
     8161 catatcaggg acttgttcgc accttcccta gaatgcatga ccaaaatccc ttaacgtgag
     8221 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8281 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8341 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8401 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     8461 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8521 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8581 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8641 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8701 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8761 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8821 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8881 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8941 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9001 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     9061 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     9121 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     9181 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9241 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9301 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     9361 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     9421 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     9481 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     9541 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     9601 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     9661 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     9721 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     9781 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     9841 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     9901 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     9961 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
    10021 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
    10081 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
    10141 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
    10201 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
    10261 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
    10321 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
    10381 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
    10441 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
    10501 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
    10561 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
    10621 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
    10681 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
    10741 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
    10801 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
    10861 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag gcaacccagc
    10921 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
    10981 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
    11041 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
    11101 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
    11161 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
    11221 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
    11281 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
    11341 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
    11401 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
    11461 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
    11521 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
    11581 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
    11641 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
    11701 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
    11761 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
    11821 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    11881 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    11941 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    12001 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    12061 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    12121 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    12181 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    12241 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    12301 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    12361 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    12421 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    12481 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    12541 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    12601 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    12661 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    12721 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    12781 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    12841 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    12901 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    12961 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    13021 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    13081 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    13141 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
    13201 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
    13261 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
    13321 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    13381 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    13441 cgacgt
//
