LOCUS       sequence-189310-       12103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express endoplasmic reticulum (ER) marker in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12103)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 12103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pMDC32-Kar2-CFP-HDEL
FEATURES             Location/Qualifiers
     source          1..12103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        444..788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(979..981,982..984,985..1695)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1024..1045)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1285..1304)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1632..1653
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      1764..2016
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2033..2049)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2033..2049)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2046..2068)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2057..2073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2081..2087,2088..2105,2106..2111))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2126..2147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2338..3014
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3081..4106
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4147..4321
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4399..4423
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4848..5642
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6928..7516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7417..7436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7670..7687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7702..7842
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7768..7787)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7928..7950
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8186..8380
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8446..9519)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9948..10577)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11878..11902
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(12090..12103,1..9)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtaaaacga cggccagtgc caagcttgca tgcctgcagg tcaacatggt ggagcacgac
       61 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
      121 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
      181 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
      241 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
      301 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
      361 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
      421 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
      481 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
      541 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
      601 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
      661 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
      721 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
      781 gagaggacct cgactctaga ggatccccgg gtaccgggcc ccccctcgag gcgcgccatg
      841 tttttcaaca gactaagcgc tggcaagctg ctggtaccac tctccgtggt cctgtacgcc
      901 cttttcgtgg taatattacc tttacagaat tctttccact cctccaatgt tttagttaga
      961 ggtgccgatg atatcgatat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1021 atcctggtcg agctggacgg cgacgtaaac ggccacaggt tcagcgtgtc cggcgagggc
     1081 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1141 cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc
     1201 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1261 caggagcgta ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1321 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1381 ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc
     1441 gccgacaagc agaagaacgg catcaaggcc cacttcaaga tccgccacaa catcgaggac
     1501 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1561 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1621 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1681 gacgagctgt acaagcacga cgaattgtaa ttaattaact agttctagag cggccgccac
     1741 cgcggtggag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     1801 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     1861 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     1921 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     1981 aattatcgcg cgcggtgtca tctatgttac tagatcggga attcgtaatc atgtcatagc
     2041 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2101 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2161 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2221 gcgcggggag aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga
     2281 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     2341 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     2401 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     2461 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     2521 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     2581 ttgatgtgaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
     2641 tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
     2701 tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg
     2761 gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg
     2821 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     2881 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
     2941 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
     3001 agaggacacg ctgaaatcac cagtctctct ctacaaatct atctctctcg agctttcgca
     3061 gatccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     3121 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     3181 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     3241 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     3301 ttccggaagt gcttgacatt ggggagttta gcgagagcct gacctattgc atctcccgcc
     3361 gttcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctacaac
     3421 cggtcgcgga ggctatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     3481 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     3541 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     3601 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     3661 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     3721 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     3781 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     3841 cggagcttgc aggatcgcca cgactccggg cgtatatgct ccgcattggt cttgaccaac
     3901 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     3961 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     4021 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     4081 gcactcgtcc gagggcaaag aaatagagta gatgccgacc gggatctgtc gatcgacaag
     4141 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat
     4201 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     4261 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat
     4321 cccccgaatt aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     4381 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     4441 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     4501 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     4561 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     4621 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc
     4681 tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg
     4741 tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct
     4801 tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga
     4861 gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag
     4921 gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa
     4981 tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc
     5041 tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct
     5101 ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg
     5161 aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca
     5221 tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt
     5281 acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat
     5341 ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg
     5401 tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg
     5461 gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg
     5521 tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac
     5581 tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt
     5641 agtacctagg gaaggtgcga ataagcgggg aaattcttct cggctgactc agtcatttca
     5701 tttcttcatg tttgagccga ttttttctcc cgtaaatgcc ttgaatcagc ctatttagac
     5761 cgtttcttcg ccatttaagg cgttatcccc agtttttagt gagatctctc ccactgacgt
     5821 atcatttggt ccgcccgaaa caggttggcc agcgtgaata acatcgccag ttggttatcg
     5881 tttttcagca accccttgta tctggctttc acgaagccga actgtcgctt gatgatgcga
     5941 aatgggtgct ccaccctggc ccggatgctg gctttcatgt attcgatgtt gatggccgtt
     6001 ttgttcttgc gtggatgctg tttcaaggtt cttaccttgc cggggcgctc ggcgatcagc
     6061 cagtccacat ccacctcggc cagctcctcg cgctgtggcg ccccttggta gccggcatcg
     6121 gctgagacaa attgctcctc tccatgcagc agattaccca gctgattgag gtcatgctcg
     6181 ttggccgcgg tggtgaccag gctgtgggtc aggccactct tggcatcgac accaatgtgg
     6241 gccttcatgc caaagtgcca ctgattgcct ttcttggtct gatgcatctc cggatcgcgt
     6301 tgctgctctt tgttcttggt cgagctgggt gcctcaatga tggtggcatc gaccaaggtg
     6361 ccttgagtca tcatgacgcc tgcttcggcc agccagcgat tgatggtctt gaacaattgg
     6421 cgggccagtt gatgctgctc cagcaggtgg cggaaattca tgatggtggt gcggtccggc
     6481 aaggcgctat ccagggataa ccgggcaaac agacgcatgg aggcgatttc gtacagagca
     6541 tcttccatcg cgccatcgct caggttgtac caatgctgca tgcagtgaat gcgtagcatg
     6601 gtttccagcg gataaggtcg ccggccatta ccagccttgg ggtaaaacgg ctcgatgact
     6661 tccaccatgt tttgccatgg cagaatctgc tccatgcggg acaagaaaat ctcttttctg
     6721 gtctgacggc gcttactgct gaattcactg tcggcgaagg taagttgatg actcatgatg
     6781 aaccctgttc tatggctcca gatgacaaac atgatctcat atcagggact tgttcgcacc
     6841 ttccctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     7081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7681 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     7741 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7801 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     7861 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     7921 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     7981 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     8041 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     8101 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     8161 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     8221 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     8281 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     8341 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
     8401 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     8461 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     8521 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     8581 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     8641 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     8701 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     8761 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     8821 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     8881 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     8941 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
     9001 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
     9061 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
     9121 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
     9181 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
     9241 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
     9301 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
     9361 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
     9421 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
     9481 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
     9541 gttccagtgc cattgcaggg ccggcaggca acccagccgc ttacgcctgg ccaaccgccc
     9601 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
     9661 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
     9721 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
     9781 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
     9841 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
     9901 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
     9961 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    10021 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    10081 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    10141 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    10201 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    10261 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    10321 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    10381 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    10441 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    10501 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    10561 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    10621 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    10681 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    10741 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    10801 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    10861 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    10921 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    10981 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    11041 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    11101 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    11161 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    11221 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    11281 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    11341 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    11401 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    11461 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    11521 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    11581 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    11641 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    11701 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    11761 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    11821 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
    11881 tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat
    11941 ctgatccaag ctcaagctgc tctagcattc gccattcagg ctgcgcaact gttgggaagg
    12001 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    12061 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgt
//
