LOCUS       sequence-189316-        9144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses estradiol-inducible synthetic transcription
            factor in yeast. It activates transcription of promoters containing 
            Zif268 binding sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Aranda-Diaz A, Mace K, Zuleta I, Harrigan P, El-Samad H
  TITLE     Robust Synthetic Circuits for Two-Dimensional Control of Gene 
            Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251.
  PUBMED    27930885
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251."
COMMENT     Sequence Label: pHES795
FEATURES             Location/Qualifiers
     source          1..9144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..353
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1172..1369
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    2014..2032
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2035..2053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             2388..3323
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2436..3326
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        complement(5147..5165)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5186..5202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5186..5202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5199..5221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5210..5226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5234..5240,5241..5258,5259..5264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5279..5300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5417..5434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5588..6176)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5668..5687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6347..7138,7139..7207))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6970..6989
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7208..7312)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7397..7415
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7453..7475)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7575..7594
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7950..8405)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8087..8108)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8299..8318
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8531..8553
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8545..8562
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8546..8562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8569..8588
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8569..8587
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca
       61 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt
      121 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcgg
      181 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc
      241 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct
      301 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatgacag
      361 tcaacactaa gacctatagt gagagagcag aaactcatgc ctcaccagta gcacaacgat
      421 tatttcgatt aatggaactg aagaaaacca atttatgtgc atcaattgat gttgatacca
      481 ctaaggaatt ccttgaatta attgataaat tgggtcctta tgtatgctta atcaagactc
      541 atattgatat aatcaatgat ttttcctatg aatccactat tgaaccatta ttagaacttt
      601 cacgtaaaca tcaatttatg atttttgaag atagaaaatt tgctgatatt ggtaataccg
      661 tgaagaaaca atatattggt ggagtttata aaattagtag ttgggcagat attactaatg
      721 ctcatggtgt cactgggaat ggagtagttg aaggattaaa acagggagct aaagaaacca
      781 ccaccaacca agagccaaga gggttattga tgttagctga attatcatca gtgggatcat
      841 tagcatatgg agaatattct caaaaaactg ttgaaattgc taaatccgat aaggaatttg
      901 ttattggatt tattgcccaa cgtgatatgg gtggacaaga agaaggattt gattggctta
      961 ttatgacacc tggagttgga ttagatgata aaggtgatgg attaggacaa caatatagaa
     1021 ctgttgatga agttgttagc actggaactg atattatcat tgttggtaga ggattgtttg
     1081 gtaaaggaag agatccagat attgaaggta aaaggtatag agatgctggt tggaatgctt
     1141 atttgaaaaa gactggccaa ttataatcag tactgacaat aaaaagattc ttgttttcaa
     1201 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
     1261 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
     1321 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg
     1381 atactaacgc cgccatccag tgtcgaaaac gacctcgaat tcatcgagtg gattcggctt
     1441 tgggtaccgg gcccggcaac caaacccata catcgggatt cctataatac cttcgttggt
     1501 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
     1561 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
     1621 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
     1681 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
     1741 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaatg atggaagaca
     1801 ctaaaggaaa aatttaacga caaagacagc accaacagat gtcgttgttc cagagctgat
     1861 gaggggtatc tcgaagcaca cgaaactttt tccttccttc attcacgcac actactctct
     1921 aatgagcaac ggtatacggc cttccttcca gttacttgaa tttgaaataa aaaaaagttt
     1981 gctgtcttgc tatcaagtat aaatagacct gaatccctat cagtgataga gatctcccta
     2041 tcagtgatag agattcttcc ttgtttcttt ttctgcaggc tagcaataaa atgggtaccc
     2101 gcccatatgc ttgccctgtc gagtcctgcg atcgccgctt ttctcgctcg gatgagctta
     2161 cccgccatat ccgcatccat accggtcaga agcccttcca gtgtcgaatc tgcatgcgta
     2221 acttcagtcg tagtgaccac cttaccaccc acatccgcac ccacacaggc gagaagcctt
     2281 ttgcctgtga catttgtggg aggaagtttg ccaggagtga tgaacgcaag aggcatacca
     2341 aaatccatac aggtagatcc ggtgacggtg ctggtttaat taactctgct ggagacatga
     2401 gagctgccaa cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg
     2461 ccttgtccct gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac
     2521 tctattccga gtatgatcct accagaccct tcagtgaagc ttcgatgatg ggcttactga
     2581 ccaacctggc agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct
     2641 ttgtggattt gaccctccat gatcaggtcc accttctaga atgtgcctgg ctagagatcc
     2701 tgatgattgg tctcgtctgg cgctccatgg agcacccagt gaagctactg tttgctccta
     2761 acttgctctt ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca
     2821 tgctgctggc tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt
     2881 gcctcaaatc tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga
     2941 agtctctgga agagaaggac catatccacc gagtcctgga caagatcaca gacactttga
     3001 tccacctgat ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc
     3061 tcctcctcat cctctcccac atcaggcaca tgagtaacaa aggcatggag catctgtaca
     3121 gcatgaagtg caagaacgtg gtgcccctct atgacctgct gctggagatg ctggacgccc
     3181 accgcctaca tgcgcccact agccgtggag gggcatccgt ggaggagacg gaccaaagcc
     3241 acttggccac tgcgggctct acttcatcgc attccttgca aaagtattac atcacggggg
     3301 aggcagaggg tttccctgcc acagtcgcgg ccgcaggtga cggtgctggt ttaattaaca
     3361 tgacggtcga ccatgatttc aatagcgaag atattttatt ccccatagaa agcatgagta
     3421 gtatacaata cgtggagaat aataacccaa ataatattaa caacgatgtt atcccgtatt
     3481 ctctagatat caaaaacact gtcttagata gtgcggatct caatgacatt caaaatcaag
     3541 aaacttcact gaatttgggg cttcctccac tatctttcga ctctccactg cccgtaacgg
     3601 aaacgatacc atccactacc gataacagct tgcatttgaa agctgatagc aacaaaaatc
     3661 gcgatgcaag aactattgaa aatgatagtg aaattaagag tactaataat gctagtggct
     3721 ctggggcaaa tcaatacaca actcttactt caccttatcc tatgaacgac attttgtaca
     3781 acatgaacaa tccgttacaa tcaccgtcac cttcatcggt acctcaaaat ccgactataa
     3841 atcctcccat aaatacagca agtaacgaaa ctaatttatc gcctcaaact tcaaatggta
     3901 atgaaactct tatatctcct cgagcccaac aacatacgtc cattaaagat aatcgtctgt
     3961 ccttacctaa tggtgctaat tcgaatcttt tcattgacac taacccaaac aatttgaacg
     4021 aaaaactaag aaatcaattg aactcagata caaattcata ttctaactcc atttctaatt
     4081 caaactccaa ttctacgggt aatttaaatt ccagttattt taattcactg aacatagact
     4141 ccatgctaga tgattacgtt tctagtgatc tcttattgaa tgatgatgat gatgacacta
     4201 atttatcacg ccgaagattt agcgacgtta taacaaacca agagctctaa gcaaatagct
     4261 aaattatata cgaattaata ttatgattaa gtgtttacgt gagtgcgata tttttattac
     4321 tatcttatac agttgtatat actctataaa atgagttgtc tattaattaa cgcgatgaat
     4381 gctttctggg tttacctctc caacaactct agtttacttc tcaatacatt caattgtatt
     4441 tgatttgtca atacttcatc attaatcaat tctatagttt tgtttttctc gtttatttcc
     4501 aaatttaatg catcaatttt attattcaat ttgtcgttga ttttggttaa tgattttatg
     4561 gtttgatctc tggcattgat tgtttgtgtt agtttttcat tattgataat taaattattt
     4621 aagttagtta tcaactcggt gttttcaagt ttcaagtttt caatttcttt agagtttatt
     4681 agatttgtca aagtttctga attgcttgat tggtccggcc caggtattgt tagcggtttg
     4741 aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt agcagaattg
     4801 tcatgcaagg gctccctatc tactggagaa tatactaagg gtactgttga cattgcgaag
     4861 agcgacaaag attttgttat cggctttatt gctcaaagag acatgggtgg aagagatgaa
     4921 ggttacgatt ggttgattat gacacccggt gtgggtttag atgacaaggg agacgcattg
     4981 ggtcaacagt atagaaccgt ggatgatgtg gtctctacag gatctgacat tattattgtt
     5041 ggaagaggac tatttgcaaa gggaagggat gctaaggtag agggtgaacg ttacagaaaa
     5101 gcaggctggg gtttaaacat ttaaatggcc ggcccagctt ttgttccctt tagtgagggt
     5161 taattccgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5221 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5281 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5341 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5401 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5461 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5521 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5581 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5641 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5701 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5761 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5821 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5881 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5941 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6001 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6061 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6121 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6181 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6241 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6301 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6361 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6421 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6481 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6541 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6601 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6661 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6721 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6781 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6841 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6901 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6961 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7021 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7081 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7141 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7201 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7261 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7321 cccgaaaagt gccacctgac gtcctcggcg cgccgacgtc taagaaacca ttattatcat
     7381 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     7441 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     7501 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     7561 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ccaccttttc
     7621 aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat
     7681 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     7741 atacgcatat tgggtcagca gtacagaacc gtcgacgaag ttgtaagtgg tggatcagat
     7801 atcatcattg ttggcagagg acttttcgcc aagggtagag atcctaatac taaactcaca
     7861 aattagagct tcaatttaat tatatcagtt attaccctat gcggtgtgaa ataccgcaca
     7921 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     7981 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     8041 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     8101 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     8161 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     8221 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     8281 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     8341 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     8401 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     8461 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     8521 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca
     8581 ctataggggg ccagcgtggc cgtttaaaca gaacgaagga aggagcacag acttagattg
     8641 gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa
     8701 ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag
     8761 gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa
     8821 aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta
     8881 gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat
     8941 ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta
     9001 ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg
     9061 ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt ggtggtttag
     9121 cttgcctcgt ccccgccggg tcac
//
