LOCUS       sequence-189604-        1863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 8a of EMMA. Functional category: 
            Signal peptide for subcellular targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1897 HC_Kan_C-KDEL_8a
FEATURES             Location/Qualifiers
     source          1..1863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      203..232
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      254..841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     742..761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(853..880)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(904..1719)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1627..1646
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1720..1822)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1823..1854)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ggaggtcagg tatgatttaa atggtcagta ttgagcgata tctagagaat tcgtcacgtc
       61 tcgaggcaag gacgagctgt aagcggccgc gactctagat cataatcagc cataccaatc
      121 ccgagacgaa aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta
      181 actggactgc aatcaactca ctggctcacc ttcacgggtg ggcctttctt cggtagaaaa
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      421 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      481 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      541 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      601 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      661 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      721 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      781 ccacctctga cttgagcatc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      841 aaacgccagc aacgcagaaa ggcccacccg aaggtgagcc aggtgattac atttgggccc
      901 tcattagaaa aactcatcga gcatcaagtg aaactgcaat ttattcatat caggattatc
      961 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     1021 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     1081 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     1141 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg
     1201 ccagccatta cgctcgtcat caaaatcact cgcaccaacc aaaccgttat tcattcgtga
     1261 ttgcgcctga gcgagacgaa atacgcgatc gccgttaaaa ggacaattac aaacaggaat
     1321 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     1381 atattcttct aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc
     1441 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     1501 gtttagcctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     1561 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     1621 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     1681 cggcctcgag caagacgttt cccgttgaat atggctcatt ttagcttcct tagctcctga
     1741 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     1801 ggaacctctt acgtgccgat caagtcaaaa gcctccggtc ggaggctttt gactttctgc
     1861 tat
//
