LOCUS       sequence-189620-        1850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 20 of EMMA. Functional category: 
            Peptide linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2060 HC_Kan_Linker3_p20
FEATURES             Location/Qualifiers
     source          1..1850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      190..221
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        222..324
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             325..1140
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(398..417)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1164..1191
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1203..1790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1283..1302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1812..1841)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 attgcagtcc agttacgctg gagtctgagg ctcgtcctga atgatatcaa gcttgaattc
       61 gttacgtctc gagcggaggc ggaggaagcg gcggaggagg ctcagccagc gctggacgag
      121 acgaagacga attctctaga tatcgctcaa tactgaccat ttaaatcata cctgacctcc
      181 atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca cgtaagaggt
      241 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
      301 ttttcaggag ctaaggaagc taaaatgagc catattcaac gggaaacgtc ttgctcgagg
      361 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
      421 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
      481 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcaggcta
      541 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
      601 gatgcatggt tactcaccac tgcgatccca gggaaaacag cattccaggt attagaagaa
      661 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
      721 tcgattcctg tttgtaattg tccttttaac ggcgatcgcg tatttcgtct cgctcaggcg
      781 caatcacgaa tgaataacgg tttggttggt gcgagtgatt ttgatgacga gcgtaatggc
      841 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca
      901 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
      961 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     1021 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     1081 attgataatc ctgatatgaa taaattgcag tttcacttga tgctcgatga gtttttctaa
     1141 tgagggccca aatgtaatca cctggctcac cttcgggtgg gcctttctgc gttgctggcg
     1201 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgatgctc aagtcagagg
     1261 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1321 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1381 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1441 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1501 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1561 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1621 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1681 acctcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1741 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1801 tgattttcta ccgaagaaag gcccacccgt gaaggtgagc cagtgagttg
//
