LOCUS       sequence-189645-        3095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMYA5 (NM_153610, nt 12205 to 12279) in pBSSK+.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Hsiung A, Naya FJ, Chen X, Shiang R
  TITLE     A schizophrenia associated CMYA5 allele displays differential 
            binding with desmin.
  JOURNAL   J Psychiatr Res. 2019 Apr;111:8-15. doi: 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10.
  PUBMED    30658136
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Psychiatr
            Res."; date: "2019-04"; volume: "111:8-15. doi"; pages: " 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10"
COMMENT     Sequence Label: CMYA5/pBSSK+
FEATURES             Location/Qualifiers
     source          1..3095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     353..370
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    487..508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(523..528,529..546,547..553)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    561..577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     566..588
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     585..601
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     585..601
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             597..1076
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSIEFLVP
                     YRIPMVNEGVHPAFALEKPGKCTLHLGIEPPDSVRHKEFNQAYRYRRPRGGARYPIRPI
                     VSRITRAHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA"
     primer_bind     620..640
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        622..640
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     677..693
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(861..877)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(862..878)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(902..921)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(903..921)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(931..948)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(931..947)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(940..962)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1089..1544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1176..1195)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1386..1407
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1570..1674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1675..1743,1744..2535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1893..1912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2706..3095,1..199)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
       61 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      241 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      301 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      361 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      421 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      481 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      541 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      601 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgattgaat
      721 tccttgtgcc ttacagaatc cccatggtta atgagggtgt ccaccctgcc tttgccctgg
      781 agaaacctgg aaaatgtact ttgcacctgg ggatagagcc cccggattct gtaaggcaca
      841 aggaattcaa tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc
      901 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
      961 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1021 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1081 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1141 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1201 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1261 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     1321 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     1381 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     1441 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     1501 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     1561 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1621 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1681 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1741 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1801 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1861 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     1921 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1981 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2041 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2101 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2161 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2221 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2281 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2341 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2401 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2461 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2521 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2581 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2641 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2701 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2761 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2821 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2881 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2941 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3001 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3061 acgacctaca ccgaactgag atacctacag cgtga
//
