LOCUS       sequence-189854-        9727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For phycocyanobilin production in Saccharomyces cerevisiae. Contains
            integrative cassette for the yeast his1-Delta-200 locus. Encodes 
            constitutively expressed HY1 and PcyA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Hochrein L, Machens F, Messerschmidt K, Mueller-Roeber B
  TITLE     PhiReX: a programmable and red light-regulated protein expression 
            switch for yeast.
  JOURNAL   Nucleic Acids Res. 2017 Sep 6;45(15):9193-9205. doi: 
            10.1093/nar/gkx610.
  PUBMED    28911120
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-09-6- 6"; volume: "45"; issue: 
            "15"; pages: "9193-9205"
COMMENT     Sequence Label: pRL_GI_PCB28
FEATURES             Location/Qualifiers
     source          1..9727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(724..1527)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(1528..1743)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      2344..3221
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     3392..3409
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(3567..4361)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3567..4361)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3678..3697)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4288..4307
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4362..4466)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4776..4877
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             4878..5552
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     terminator      complement(5797..6062)
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     promoter        7835..8539
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     terminator      9293..9458
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(9348..9372)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcatgata
      661 taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt
      721 tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg
      781 ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat
      841 gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc
      901 cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc
      961 acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc
     1021 aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa
     1081 catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac
     1141 aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta
     1201 tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc
     1261 gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat
     1321 ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc
     1381 ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt
     1441 ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc
     1501 acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg tttttgttct
     1561 gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat
     1621 atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga
     1681 atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt
     1741 gaaaagctag cttatcgatg ataagctgtc aaacatgaga attaattcca cggactatag
     1801 actataccta gtatactccg tctactgtac gatacacttc cgctcaggtc cttgtccttt
     1861 aacgaggcct taccactctt ttgttactct attgatccag ctcagcaaag gcagtgtgat
     1921 ctaagattct atcttcgcga tgtagtaaaa ctagctagac cgagaaagag actagaaatg
     1981 caaaaggcac ttctacaatg gctgccatca ttattatccg atgtgacgct gcagcttctc
     2041 aatgatattc gaatacgctt tgaggagata cagcctaata tccgacaaac tgttttacag
     2101 atttacgatc gtacttgtta cccatcattg aattttgaac atccgaacct gggagttttc
     2161 cctgaaacag atagtatatt tgaacctgta taataatata tagtctagcg ctttacggaa
     2221 gacaatgtat gtatttcggt tcctggagaa actattgcat ctattgcata ggtaatcttg
     2281 cacgtcgcat ccccggttca ttttctgcgt ttccatcttg cacttcaata gcatatcttt
     2341 gttaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
     2401 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
     2461 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
     2521 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
     2581 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
     2641 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
     2701 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
     2761 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
     2821 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
     2881 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
     2941 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
     3001 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
     3061 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
     3121 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
     3181 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tgggaagctc caccccggtt
     3241 gataatcaga aaagccccaa aaacaggaag attgtataag caaatattta aattgtaatt
     3301 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3361 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3421 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3481 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3541 atatatgagt aaacttggtc tgacagtcag aagaactcgt caagaaggcg atagaaggcg
     3601 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     3661 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     3721 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     3781 ggcaagcagg catcgccatg agtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     3841 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     3901 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     3961 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     4021 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     4081 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     4141 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     4201 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     4261 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     4321 gcggccggag aacctgcgtg caatccatct tgttcaatca tactcttcct ttttcaatat
     4381 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgga
     4501 cggatcgctt gcctgtaact tacacgcgcc tcgtggcgcg cccctgcagg atttaaatgg
     4561 ccggccgttt aaactcttca ttcaacgttt cccattgttt ttttctacta ttgctttgct
     4621 gtgggaaaaa cttatcgaaa gatgacgact ttttcttaat tctcgtttta agagcttggt
     4681 gagcgctagg agtcactgcc aggtatcgtt tgaacacggc attagtcagg gaagtcataa
     4741 cacagtcctt tcccgcaatt ttctttttct attacaattc ggtcgaaaaa agaaaaggag
     4801 agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca
     4861 caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga
     4921 aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact
     4981 tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga
     5041 aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg
     5101 gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg
     5161 atatcgtcca actgcacgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc
     5221 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac
     5281 agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt
     5341 tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgag agcttacatt
     5401 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg
     5461 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa
     5521 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt
     5581 attgtttgtg cacttgccct agcgaaccag gcttatgccc agcatcgttg caagcagact
     5641 tttgttgcaa gtgggatgag cttggagcag gaagaataca ctatactgga tctaaagagt
     5701 acaatagatg gataagaata ttggcagcgc aaaaaggctt caagcttaca caacacggtt
     5761 tatttcgaaa taatatcctt ctcgaaagct ttaacgaacg cagaattttc gagttattaa
     5821 acttaaaata cgctgaaccc gaacatagaa atatcgaatg ggaaaaaaaa actgcataaa
     5881 ggcattaaaa gaggagcgaa ttttttttta ataaaaatct taataatcat taaaagataa
     5941 ataatagtct atatatacgt atataaataa aaaatattca aaaaataaaa taaactatta
     6001 ttttagcgta aaggatgggg aaagagaaaa gaaaaaaatt gatctatcga tttcaattca
     6061 attctcagga caatatgaga cgaagtatct caccagaata cttgaacgat ttctcagtct
     6121 cttccaaaca atgattcttt tcttctctag tccactcctc tgcaaccttg ttcagtttct
     6181 ccctaacgtt ctgcaacaat tgagaaagtt cgccgtccca tttgtagaac tcgagttctt
     6241 tattatcgag tatccgctct gccacctttc tgccaatcat tcgtccacca gcactatgag
     6301 caaagtagat gttgtagaag tgacaaatga atgcttgagg atccttctct gctaattcct
     6361 ttaaatattg agaatatgtt ttaccaggag ctgttggttc tggaatctcg taaccttgtt
     6421 ccttgaacca ctccaaatcc gtggataatt tctccgctct ttccagcccc gtgttcttga
     6481 actcggcata agttgggaaa ttggagtctt gaataatcag ttcaagagta tcataaacca
     6541 atttactatc cacaagaaac ctcaagtaac cttcaacagt aggttcccat ttagcaacag
     6601 gacgttcctc aatagattta gtctctttct caccttcctt agcttgatct ttagtatgaa
     6661 gtctcatagc cacaaacctc atctcctcca caaaaccctt tgattctcca ggatacctct
     6721 tcttctgctt ctctgccgca gtagtagccg caaccaccac taaagacggc gacttgttca
     6781 tggtcatact cagaatctga atcctaggtc gaaggaaaag tgggttcggt gaggaagaag
     6841 aaaattggaa tcttgagagt tggggattct tgaatatgga taaggatgaa gaaatcggag
     6901 ctaaatacgc catcattgtt ttatatttgt tgtaaaaagt agataattac ttccttgatg
     6961 atctgtaaaa aagagaaaaa gaaagcatct aagaacttga aaaactacga attagaaaag
     7021 accaaatatg tatttcttgc attgaccaat ttatgcaagt ttatatatat gtaaatgtaa
     7081 gtttcacgag gttctactaa actaaaccac ccccttggtt agaagaaaag agtgtgtgag
     7141 aacaggctgt tgttgtcaca cgattcggac aattctgttt gaaagagaga gagtaacagt
     7201 acgatcgaac gaactttgct ctggagatca cagtgggcat catagcatgt ggtactaaac
     7261 cctttcccgc cattccagaa ccttcgattg cttgttacaa aacctgtgag ccgtcgctag
     7321 gaccttgttg tgtgacgaaa ttggaagctg caatcaatag gaagacagga agtcgagcgt
     7381 gtctgggttt tttcagtttt gttctttttg caaacaaatc acgagcgacg gtaatttctt
     7441 tctcgataag aggccacgtg ctttatgagg gtaacatcaa ttcaagaagg agggaaacac
     7501 ttcctttttc tggccctgat aatagtatga gggtgaagcc aaaataaagg attcgcgccc
     7561 aaatcggcat ctttaaatgc aggtatgcga tagttcctca ctctttcctt actcacgagt
     7621 aattcttgca aatgcctatt atgcagatgt tataatatct gtgcgcacta gtcggagatt
     7681 atcgagcata ctatcacgtc ggacaatatg gaagggaact ttacacttct cctatgcaca
     7741 tatattaatt aaagtccaat gctagtagag aaggggggta acacccctcc gcgctctttt
     7801 ccgatttttt tctaaaccgt ggaatatttc ggatatcctt ttgttgtttc cgggtgtaca
     7861 atatggactt cctcttttct ggcaaccaaa cccatacatc gggattccta taataccttc
     7921 gttggtctcc ctaacatgta ggtggcggag gggagatata caatagaaca gataccagac
     7981 aagacataat gggctaaaca agactacacc aattacactg cctcattgat ggtggtacat
     8041 aacgaactaa tactgtagcc ctagacttga tagccatcat catatcgaag tttcactacc
     8101 ctttttccat ttgccatcta ttgaagtaat aataggcgca tgcaacttct tttctttttt
     8161 tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa aaaatgatgg
     8221 aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg ttgttccaga
     8281 gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc acgcacacta
     8341 ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg aaataaaaaa
     8401 aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct tttgtttcct
     8461 cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat ttcaagctat
     8521 accaagcata caatcaacta tctcaatggc tgttactgat ttgtctttga ctaactcctc
     8581 tttgatgcca actttgaacc caatgattca acaattggct ttggctattg ctgcttcttg
     8641 gcaatctttg ccattgaaac catatcaatt gcctgaagat ttgggttacg ttgaaggtag
     8701 attggaaggt gaaaagttgg tcattgaaaa cagatgttac caaaccccac aattcagaaa
     8761 gatgcatttg gaattggcta aggttggtaa gggtttggat atcttgcatt gtgttatgtt
     8821 cccagaacca ttatacggtt tgcctttgtt tggttgtgat atagttgctg gtccaggtgg
     8881 tgtttctgct gctattgcag atttgtctcc aactcaatct gatagacaat tgccagctgc
     8941 ttaccaaaaa tctttggctg aattgggtca accagaattc gaacaacaaa gagaattgcc
     9001 accttggggt gaaattttct ctgaatactg cttgttcatc agaccatcta acgttaccga
     9061 agaagaaaga ttcgttcaaa gagttgtcga cttcttgcaa atccattgcc atcaatctat
     9121 cgttgctgaa ccattgtctg aagctcaaac tttggaacat agacaaggtc aaattcacta
     9181 ctgccagcaa caacaaaaaa acgacaagac cagaagagtt ttggaaaagg cttttggtga
     9241 agcttgggct gaaagatata tgtcccaagt tttgttcgac gttattcaat aagcgaattt
     9301 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
     9361 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta
     9421 ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca ccgctttttc
     9481 tttttttttc tcttgaactc gagaaaaaaa atataaaaga gatggaggaa cgggaaaaag
     9541 ttagttgtgg tgataggtgg caagtggtat tccgtaagaa caacaagaaa agcatttcat
     9601 attatggctg aactgagcga acaagtgcaa aatttaagca tcaacgacaa caacgagaat
     9661 ggttatgttc ctatttaaat ggcgcgccgt ttaaaccctg caggggccgg ccccgtagaa
     9721 aagatca
//
