LOCUS       sequence-189925-        4941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing NFAT2 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-NFAT2_isoform1
FEATURES             Location/Qualifiers
     source          1..4941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2252..2351)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2368..2387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2369..2387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2392..2408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2392..2408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2839..3630
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3723..4311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4212..4231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4465..4482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4641..4668)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4760..4846)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4895..4917
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4909..4926
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4910..4926
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatgccaag caccagcttt
      121 ccagtccctt ccaagtttcc acttggccct gcggctgcgg tcttcgggag aggagaaact
      181 ttggggcccg cgccgcgcgc cggcggcacc atgaagtcag cggaggaaga acactatggc
      241 tatgcatcct ccaacgtcag ccccgccctg ccgctcccca cggcgcactc caccctgccg
      301 gccccgtgcc acaaccttca gacctccaca ccgggcatca tcccgccggc ggatcacccc
      361 tcggggtacg gagcagcttt ggacggtggg cccgcgggct acttcctctc ctccggccac
      421 accaggcctg atggggcccc tgccctggag agtcctcgca tcgagataac ctcgtgcttg
      481 ggcctgtacc acaacaataa ccagtttttc cacgatgtgg aggtggaaga cgtcctccct
      541 agctccaaac ggtccccctc cacggccacg ctgagtctgc ccagcctgga ggcctacaga
      601 gacccctcgt gcctgagccc ggccagcagc ctgtcctccc ggagctgcaa ctcagaggcc
      661 tcctcctacg agtccaacta ctcgtacccg tacgcgtccc cccagacgtc gccatggcag
      721 tctccctgcg tgtctcccaa gaccacggac cccgaggagg gctttccccg cgggctgggg
      781 gcctgcacac tgctgggttc cccgcggcac tccccctcca cctcgccccg cgccagcgtc
      841 actgaggaga gctggctggg tgcccgctcc tccagacccg cgtccccgtg caacaagagg
      901 aagtacagcc tcaacggccg gcagccgccc tactcacccc accactcgcc cacgccgtcc
      961 ccgcacggct ccccgcgggt cagcgtgacc gacgactcgt ggttgggcaa caccacccag
     1021 tacaccagct cggccatcgt ggccgccatc aacgcgctga ccaccgacag cagcctggac
     1081 ctgggagatg gcgtccctgt caagtcccgc aagaccaccc tggagcagcc gccctcagtg
     1141 gcgctcaagg tggagcccgt cggggaggac ctgggcagcc ccccgccccc ggccgacttc
     1201 gcgcccgaag actactcctc tttccagcac atcaggaagg gcggcttctg cgaccagtac
     1261 ctggcggtgc cgcagcaccc ctaccagtgg gcgaagccca agcccctgtc ccctacgtcc
     1321 tacatgagcc cgaccctgcc cgccctggac tggcagctgc cgtcccactc aggcccgtat
     1381 gagcttcgga ttgaggtgca gcccaagtcc caccaccgag cccactacga gacggagggc
     1441 agccgggggg ccgtgaaggc gtcggccgga ggacacccca tcgtgcagct gcatggctac
     1501 ttggagaatg agccgctgat gctgcagctt ttcattggga cggcggacga ccgcctgctg
     1561 cgcccgcacg ccttctacca ggtgcaccgc atcacaggga agaccgtgtc caccaccagc
     1621 cacgaggcca tcctctccaa caccaaagtc ctggagatcc cactcctgcc ggagaacagc
     1681 atgcgagccg tcattgactg tgccggaatc ctgaaactca gaaactccga cattgaactt
     1741 cggaaaggag agacggacat cgggaggaag aacacacggg tacggctggt gttccgcgtt
     1801 cacgtcccgc aacccagcgg ccgcacgctg tccctgcagg tggcctccaa ccccatcgaa
     1861 tgctcccagc gctcagctca ggagctgcct ctggtggaga agcagagcac ggacagctat
     1921 ccggtcgtgg gcgggaagaa gatggtcctg tctggccaca acttcctgca ggactccaag
     1981 gtcattttcg tggagaaagc cccagatggc caccatgtct gggagatgga agcgaaaact
     2041 gaccgggacc tgtgcaagcc gaattctctg gtggttgaga tcccgccgtt tcggaatcag
     2101 aggataacca gccccgttca cgtcagtttc tacgtctgca acgggaagag aaagcgaagc
     2161 cagtaccagc gtttcaccta ccttcccgcc aacggtaacg ccatctttct aaccgtaagc
     2221 cgtgaacatg agcgcgtggg gtgctttttc ttgccaactt tcttgtacaa agttggcatt
     2281 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2341 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     2401 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2461 aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta
     2521 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca
     2581 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     2641 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     2701 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     2761 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     2821 aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     2881 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     2941 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     3001 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     3061 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     3121 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     3181 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     3241 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     3301 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     3361 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     3421 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     3481 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     3541 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     3601 ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt
     3661 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3721 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3781 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3841 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3901 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3961 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4021 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4081 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4141 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4201 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4261 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4321 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4381 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4441 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4501 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4561 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     4621 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     4681 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     4741 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     4801 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     4861 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4921 gccagtctta agctcgggcc c
//
