LOCUS       sequence-189928-        5025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF366 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF366
FEATURES             Location/Qualifiers
     source          1..5025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2336..2435)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2452..2471)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2453..2471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2476..2492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2476..2492)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2923..3714
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3807..4395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4296..4315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4549..4566
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4725..4752)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4844..4930)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4979..5001
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4993..5010
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4994..5010
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatgcagaa ggaaatgaag
      121 atgatcaaag acgaggatgt gcatttcgac ttggctgtga agaagacccc ctcctttccc
      181 cactgcctgc agccagtggc ttctcgggga aaggctcccc aaagacaccc cttcccggaa
      241 gctctccgag ggccattttc ccagtttcgg tatgaacctc ccccaggaga cctagatggg
      301 ttccccgggg tcttcgaagg agcagggtct aggaaacgga agagcatgcc cacaaagatg
      361 ccctataacc accctgcaga agaagtcacc ctcgccctcc actcagagga gaacaaaaac
      421 cacggccttc ccaacctccc tttgctgttc ccgcagcccc cgcgccccaa gtatgactct
      481 cagatgatcg acctgtgcaa cgtgggcttc caattctacc gcagcctgga acactttggg
      541 ggcaagcccg tcaagcagga acccattaag cccagcgccg tgtggcccca gccaacgccc
      601 actccattcc tgcccacgcc ctacccctac taccccaaag tccacccggg cctcatgttc
      661 cccttcttcg tgccctcgtc ctcgcccttc cccttcagcc ggcacacctt cctgcccaag
      721 cagcccccgg aacctctgct gccccggaaa gccgagcccc aggagagcga ggagaccaag
      781 cagaaggtgg agagggtgga cgtgaacgtg cagatcgatg acagctacta cgtggacgtg
      841 ggcggctcgc agaagcgctg gcagtgcccc acctgcgaga agtcctacac ctccaagtac
      901 aacctggtca cccacatcct gggccacagt gggatcaagc cgcacgcgtg cacgcactgc
      961 gggaagctct tcaagcagct cagccacctg catacccaca tgctgaccca ccagggcacg
     1021 cggccccaca agtgccaggt gtgccacaag gccttcaccc agaccagcca cctgaagcgc
     1081 cacatgatgc agcacagcga ggtgaagccg cacaactgcc gcgtgtgcgg ccgcggcttt
     1141 gcctacccca gcgagctcaa ggcccacgaa gccaagcacg ccagtgggcg cgagaacatc
     1201 tgtgtggagt gcggcctcga cttccccacc ttggcccagc tgaagagaca cctcaccacg
     1261 caccggggcc ccatccagta caactgctcc gagtgcgaca agaccttcca gtacccgagc
     1321 cagctgcaga accacatgat gaagcacaag gacatccggc cctacatctg ctcagagtgt
     1381 ggcatggagt ttgtgcagcc gcaccacctc aagcagcact ccctcaccca caagggtgtg
     1441 aaggagcata agtgtgggat ttgtgggcgg gagttcaccc tgctggccaa catgaagcga
     1501 cacgtgctga tccacaccaa catccgcgcc taccagtgtc acctctgcta caagagcttc
     1561 gtgcagaagc agaccctcaa ggcacacatg atcgtccact ctgacgtgaa gcctttcaaa
     1621 tgcaagcttt gtgggaagga attcaaccgg atgcacaacc tgatgggcca catgcacctg
     1681 cactcagaca gcaaaccctt caagtgcctc tattgcccaa gcaaattcac cctgaagggg
     1741 aacctgacac gccacatgaa agtcaagcat ggagtcatgg agcggggcct tcattcccaa
     1801 ggtctgggaa gggggagaat cgccctggca cagacagccg gtgtcctgag gagtctggag
     1861 caggaggagc cctttgacct ctctcagaag cgccgggcca aggtgccggt gttccagtca
     1921 gacggggaga gtgcccaggg cagccactgc cacgaggagg aagaggagga taactgctac
     1981 gaggtggagc cctacagccc tggcctggcc ccccagagcc agcagctctg cacacccgag
     2041 gatctgtcca ccaagtcgga gcacgccccc gaggtgctgg aggaagcctg caaggaggag
     2101 aaggaggatg catccaaggg agaatgggag aagaggagca agggtgacct tggggcagag
     2161 ggcggccagg agagagactg tgccggcaga gatgagtgtc tcagtctcag ggcttttcag
     2221 agtacccggc ggggcccctc tttttctgat tacttatact tcaagcacag agatgagagt
     2281 ttgaaagaat tactggagag gaaaatggaa aaacaagcag tgcttttagg tatcttgcca
     2341 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2401 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2461 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2521 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     2581 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     2641 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     2701 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     2761 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     2821 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     2881 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
     2941 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     3001 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     3061 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     3121 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     3181 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     3241 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     3301 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     3361 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     3421 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     3481 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     3541 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     3601 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     3661 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     3721 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3781 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3901 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3961 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4021 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4081 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4141 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4201 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4261 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4321 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4381 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4441 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4501 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4561 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4621 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4681 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4741 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     4801 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     4861 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     4921 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     4981 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
