LOCUS       sequence-189970-       10708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancing the upper-part mevalonate pathway; overexpressing 
            codon-optimized Enterococcus faecalis mevalonate pathway genes under
            the control of consitutive promoters. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Peng B, Plan MR, Chrysanthopoulos P, Hodson MP, Nielsen LK, Vickers 
            CE
  TITLE     A squalene synthase protein degradation method for improved 
            sesquiterpene production in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2017 Jan;39:209-219. doi: 10.1016/j.ymben.2016.12.003. 
            Epub 2016 Dec 8.
  PUBMED    27939849
REFERENCE   2  (bases 1 to 10708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2017-01"; volume: "39:209-219. doi"; pages: " 
            10.1016/j.ymben.2016.12.003. Epub 2016 Dec 8"
COMMENT     Sequence Label: pPMVAu8
FEATURES             Location/Qualifiers
     source          1..10708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(484..504)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(484..502)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(523..539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(523..539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(536..558)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    547..563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(571..577,578..595,596..601))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    616..637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(754..771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(925..1513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1005..1024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1684..2475,2476..2544))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2307..2326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2545..2649)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2676..4018
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(3639..3686)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     4059..4077
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4115..4137)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4237..4256
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4403..4590
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             4591..5253
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 aaccaagaca gagctttagc aattttgaac gatttgagaa aacaataatg atagtattgt
       61 tcgtttttta tcatagtttt tcaaatcttc atatgaatac taggtagaaa tttggcttat
      121 cagtacgaat gaattacagt ttgactgatt tgtacacgag aattgacgta taacttcttt
      181 tgaaagtctt tgaaattaca tggtagtaca tacatatata tatatatata tatatgttat
      241 attattgtta attaaatatt attatgcgta ttttcctttt ctttattagt atagtattaa
      301 tgacagtaat aataataata atagtaacaa tatctctttt tttttttcag tgagctttta
      361 tttttttttc attgctcttc ttttggcctc ttttgttttt ttcttgattt cctccagttt
      421 catctgtttt ttctttggat cagatacaaa atctggtttg aacgcgagct ccagcttttg
      481 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
      541 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      601 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      661 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      721 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      781 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      841 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      901 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      961 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1021 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1081 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1141 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1201 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1261 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1321 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1381 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1441 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1501 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1561 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1621 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1681 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1741 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1801 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1861 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1921 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1981 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2041 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2101 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2161 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2221 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2281 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2341 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2401 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2461 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2521 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2581 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2641 ggttccgcgc acatttcccc gaaaagtgcc acctgaacga agcatctgtg cttcattttg
     2701 tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     2761 ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc tgtgcttcat
     2821 ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     2881 catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa gaatctatac
     2941 ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca aagcatctta
     3001 gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac tttttgcact
     3061 gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt ccataaaaaa
     3121 agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg cattttttca
     3181 agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac tttgtgaaca
     3241 gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt ttcttctatt
     3301 ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt cgattcactc
     3361 tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga taaacataaa
     3421 aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg ggtaggttat
     3481 atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg tttgtggaag
     3541 cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt tttttgaaag
     3601 tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata ctttctagag
     3661 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
     3721 caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
     3781 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
     3841 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
     3901 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
     3961 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcat
     4021 actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4081 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4141 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4201 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4261 gagtgcacca taaattcccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta
     4321 tcgtttgaac acggcattag tcagggaagt cataacacag tcctttcccg caattttctt
     4381 tttctattac tcttggcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat
     4441 tttcattttt ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta
     4501 tcacataatg aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa
     4561 aatgagcagg caagataaac gaaggcaaag atgacagagc agaaagccct agtaaagcgt
     4621 attacaaatg aaaccaagat tcagattgcg atctctttaa agggtggtcc cctagcgata
     4681 gagcactcga tcttcccaga aaaagaggca gaagcagtag cagaacaggc cacacaatcg
     4741 caagtgatta acgtccacac aggtataggg tttctggacc atatgataca tgctctggcc
     4801 aagcattccg gctggtcgct aatcgttgag tgcattggtg acttacacat agacgaccat
     4861 cacaccactg aagactgcgg gattgctctc ggtcaagctt ttaaagaggc cctaggggcc
     4921 gtgcgtggag taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga
     4981 gcggtggtag atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag
     5041 aaagtaggag atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct
     5101 agcagaatta ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag
     5161 agtgcgttca aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac
     5221 gatgttccct ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca
     5281 tacgatatat atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga
     5341 acagtatgat actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga
     5401 ggcgcgcttt ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc
     5461 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     5521 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     5581 caataggccg aaatcggcaa aatcccttat aaggatccac ctaggaaatc agacaatctg
     5641 tctgacgata tagagagcga agaggaagag gaagagggtg atgacagctt ggaatcatgg
     5701 ctttcccagc tttacataga ctcaagtggc gagccttcac cctcaacgtg ggccttagct
     5761 aaccttttga ctttaacggc cgttcaatgg gaatcattgt tctccaaaaa ggccactcct
     5821 cacatcagtg attctatagt taacgaagaa aagctgccct tcctggccaa aaaagacaat
     5881 ccccattcca agaaattgct ttccaaatta cttaaggaaa agcaactacc ttgcataaaa
     5941 ggcgacaact cgtcaaagat aacaagtgct actaaaagca tgctccaaaa tgctcgaaca
     6001 cttgcacaat cggagcataa tatcttagat agatgtctca agagactatc ctaaataata
     6061 ttgaatatgc acttttacta tattaatatc acgtcacacg acgcacagtg agaagtgaaa
     6121 aatttttttt caatctgaaa aaaaaaaaaa aaaaaaattt atataaacga atggtatctc
     6181 catcacattt cttttagcct cgcaacttat acttttcatc acttttcttg taatttagca
     6241 atatcccaag aacaatcatc aaaatgacca tcggtattga caaaatctcc tttttcgtcc
     6301 ctccttatta cattgacatg acagcattag cagaagccag aaacgtagac cctggtaaat
     6361 ttcatatcgg tattggtcaa gaccaaatgg ctgtaaatcc tatttcccaa gatatagtta
     6421 ccttcgctgc aaacgccgct gaagccattt taactaaaga agacaaggaa gcaatcgata
     6481 tggtcattgt aggtactgaa tcttcaattg atgaatccaa agcagccgct gttgtcttac
     6541 acagattgat gggtatacaa ccatttgcta gaagtttcga aatcaaagaa gcatgttatg
     6601 gtgctacagc aggtttgcaa ttagcaaaga atcatgtcgc cttacaccct gacaaaaagg
     6661 tattggtagt tgcagccgat attgctaaat acggtttaaa ttctggtggt gaaccaactc
     6721 aaggtgccgg tgctgttgca atgttggtcg cctcagaacc tagaatcttg gctttgaagg
     6781 aagacaacgt tatgttgact caagacatct atgatttctg gagaccaaca ggtcatccat
     6841 accctatggt tgatggtcct ttgtctaacg aaacctacat acaatcattc gctcaagttt
     6901 gggacgaaca taaaaagaga actggtttag atttcgcaga ttatgacgcc ttggctttcc
     6961 acattccata cacaaaaatg ggtaaaaagg ctttgttagc aaagatttca gatcaaaccg
     7021 aagcagaaca agaaagaata ttagccagat acgaagaatc catcatatac agtagaagag
     7081 ttggtaattt gtatacaggt tccttgtact taggtttgat aagtttgtta gaaaacgcta
     7141 ctacattgac cgcaggtaac caaatcggtt tgttttctta tggttcaggt gcagttgccg
     7201 aatttttcac tggtgaattg gtcgctggtt accaaaacca tttgcaaaag gaaacacact
     7261 tggctttgtt agataacaga accgaattat ctattgcaga atacgaagcc atgtttgctg
     7321 aaaccttgga tactgacatc gatcaaactt tagaagatga attgaagtac tccatcagtg
     7381 ctatcaataa cacagttaga tcatacagaa attaatgata gctttgatct gtagcctaag
     7441 tataaaattc tacgtatgta tatatttaca tgcaattttt tctttttcca attcatgtta
     7501 atgttcttca tcatttgata ataggccaat gatattatct actatcttct ccttttgagg
     7561 agaagctgct ttcacttcct cgttgctttt ctcttcgtat aaatacatga atttcgactt
     7621 gtccagcatt tttttatcaa tacaatgcat gtgcccatga agttctacct ccgacaaatc
     7681 tttaacaaaa tatgtcgtta tttgtgtcat gaatttcagt actcgaggta ttcaagatgt
     7741 tcttatcaaa gcacttattt tttcattata tttctatatt gatttaggga cggtacgcta
     7801 ttttctttgt accttttact atgcataaaa aaaggggaag tgttcaacaa tcgacactta
     7861 cttaatatgt tttgccgccc ttcataagag ggtgtttcta aaatttattg ggcaagaatg
     7921 agatggactc gcaccctaca taacgtttag atatttagtg ttaagttcaa aacatgcatc
     7981 aggtgcgaca tgtgttgcga ttatcatgac aatgtctcta tccgagatgc atttgtagta
     8041 tcaactgatg catattatga catgattcac atagcataca tcgtcaaaca tgatgttatt
     8101 tttttgataa atgtacggat ttaaagctgt cgaatatatt ttctgaaatt tcttggagcc
     8161 gacgcaaaat tttcaaaggt gctaaaattt ttcaagattt ctcacttttg cttggtaaca
     8221 aagaatgatg gcattgcatt tttaccaccg gtacatttaa ctgctatttc tcacgtttct
     8281 ttctctatcc ttaagtaatt cttttacagt ctaagaaaac cacgatcaaa caaataaatc
     8341 aacaatgaag actgtagtaa taatagacgc attgagaaca ccaatcggta aatataaagg
     8401 tagtttgtcc caagtttccg ctgtagactt aggtactcat gttactacac aattgttgaa
     8461 gagacactcc acaatcagtg aagaaatcga tcaagtcata ttcggtaacg tattacaagc
     8521 tggtaacggt caaaacccag ctagacaaat agcaatcaat tctggtttgt cacatgaaat
     8581 tcctgctatg acagtaaacg aagtttgtgg ttcaggcatg aaagccgtaa ttttggctaa
     8641 gcaattgatc caattgggtg aagccgaagt tttgatcgct ggtggtatag aaaacatgag
     8701 tcaagctcca aaattacaaa gattcaatta cgaaactgaa tcttacgatg cacctttctc
     8761 ttcaatgatg tatgatggtt taactgacgc attttctggt caagccatgg gtttgacagc
     8821 agaaaatgta gccgaaaagt accatgttac cagagaagaa caagatcaat tttccgttca
     8881 cagtcaattg aaagctgcac aagcacaagc cgaaggtatt ttcgctgacg aaatagcacc
     8941 attagaagtt tctggtacat tggtcgaaaa ggatgaaggt ataagaccta actccagtgt
     9001 tgaaaaattg ggtactttga agacagtttt caaggaagac ggtacagtca ccgccggtaa
     9061 tgcttctacc attaacgatg gtgctagtgc attgataatc gcttctcaag aatatgccga
     9121 agctcatggt ttgccatact tagctatcat tagagatagt gtagaagttg gtattgaccc
     9181 agcatatatg ggtatttctc ctataaaagc catccaaaag ttgttggcta gaaaccaatt
     9241 gaccactgaa gaaatcgatt tgtacgaaat taacgaagca tttgccgcta cttcaatcgt
     9301 tgtccaaaga gaattggcat tgcctgaaga aaaggttaac atctatggtg gtggtatctc
     9361 cttaggtcac gcaattggtg ccaccggtgc tagattgttg acttccttga gttaccaatt
     9421 gaaccaaaag gaaaagaaat acggtgttgc ttctttgtgc attggtggtg gtttgggttt
     9481 agcaatgttg ttagaaagac cacaacaaaa gaaaaattca agattctacc aaatgtcccc
     9541 tgaagaaaga ttggcatcat tgttgaacga aggtcaaatt tccgccgata ctaagaaaga
     9601 atttgaaaac accgcattgt cttcacaaat cgccaaccat atgatcgaaa accaaatctc
     9661 tgaaacagaa gttccaatgg gtgtcggttt gcacttaact gtagatgaaa cagactattt
     9721 ggttccaatg gctaccgaag aacctagtgt tatcgcagcc ttgtctaatg gtgcaaaaat
     9781 tgcccaaggt tttaagactg ttaaccaaca aagattgatg agaggtcaaa ttgtattcta
     9841 cgatgttgca gacgccgaat cattgatcga tgaattacaa gttagagaaa ctgaaatctt
     9901 tcaacaagct gaattatctt acccatcaat cgttaagaga ggtggtggtt tgagagattt
     9961 gcaatacaga gcttttgacg aatccttcgt cagtgtagat ttcttggttg atgtcaaaga
    10021 cgctatgggt gcaaatattg tcaacgctat gttagaaggt gtagcagaat tgtttagaga
    10081 atggttcgct gaacaaaaga ttttgttttc tatcttgtca aactatgcta cagaatctgt
    10141 agttaccatg aaaactgcaa ttccagtttc cagattatct aagggttcta acggtagaga
    10201 aatcgctgaa aagattgtct tagcctcaag atatgcttcc ttggaccctt acagagcagt
    10261 tactcataat aagggtataa tgaacggtat cgaagcagtc gtattggcca ccggtaatga
    10321 tactagagcc gtttctgctt catgtcacgc tttcgcagtc aaggaaggta gatatcaagg
    10381 tttgacatca tggaccttag atggtgaaca attgattggt gaaatatccg ttccattggc
    10441 cttagctact gtcggtggtg ctacaaaagt attacctaag agtcaagctg ctgctgattt
    10501 gttagctgta acagacgcaa aagaattgtc aagagttgtc gctgctgttg gtttggccca
    10561 aaatttggcc gctttaagag ctttggtctc agaaggtatt caaaagggtc atatggcatt
    10621 acaagccaga tccttggcta tgacagttgg tgcaaccggt aaagaagtcg aagcagtagc
    10681 ccaacaattg aagagacaaa agactatg
//
